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AB113860

Recombinant Human GMDS protein (His tag N-Terminus)

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Recombinant Human GMDS protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 372 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

대체 명칭 보기

GDP-D-mannose dehydratase, GMD, GMDS

1 이미지
SDS-PAGE - Recombinant Human GMDS protein (His tag N-Terminus) (AB113860)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human GMDS protein (His tag N-Terminus) (AB113860)

15% SDS-PAGE analysis of ab113860 (3μg)

주요 정보

Purity

>95% SDS-PAGE

ab113860 was purified using conventional chromatography.

발현 시스템

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 8 Constituents: 30% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF

storage-buffer

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보완 제품

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Recombinant Human C-Reactive Protein/CRP Protein

SDS-PAGE - Recombinant Human C-Reactive Protein/CRP Protein (AB283925)

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Primary Antibodies

AB97630

Anti-GMDS antibody

  • 0
  • 0
Proteins & Peptides

AB283919

Recombinant Human Cystatin C Protein (Active)

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Proteins & Peptides

AB132084

Recombinant Human DCTN1/p150-glued protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human DCTN1/p150-glued protein (GST tag N-Terminus) (AB132084)

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  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"MGSSHHHHHHSSGLVPRGSHMAHAPARCPSARGSGDGEMGKPRNVALITGITGQDGSYLAEFLLEKGYEVHGIVRRSSSFNTGRIEHLYKNPQAHIEGNMKLHYGDLTDSTCLVKIINEVKPTEIYNLGAQSHVKISFDLAEYTADVDGVGTLRLLDAVKTCGLINSVKFYQASTSELYGKVQEIPQKETTPFYPRSPYGAAKLYAYWIVVNFREAYNLFAVNGILFNHESPRRGANFVTRKISRSVAKIYLGQLECFSLGNLDAKRDWGHAKDYVEAMWLMLQNDEPEDFVIATGEVHSVREFVEKSFLHIGKTIVWEGKNENEVGRCKETGKVHVTVDLKYYRPTEVDFLQGDCTKAKQKLNWKPRVAFDELVREMVHADVELMRTNPNA","proteinLength":"Full Length","predictedMolecularWeight":"44.1 kDa","actualMolecularWeight":null,"aminoAcidEnd":372,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O60547","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The GMDS protein also known as GDP-mannose 46-dehydratase plays an important role in catalyzing the conversion of GDP-mannose to GDP-4-keto-6-deoxymannose one step in the biosynthesis of GDP-fucose. It has an approximate mass of 39 kDa and predominantly expresses in the liver kidneys and certain immune cells. The GMDS enzyme operates in the cytosol contributing to cellular metabolism and energy balance providing substrates necessary for further enzymatic conversions.
Biological function summary

GMDS contributes significantly to the fucosylation process a modification critical for the functioning of various glycoproteins and glycolipids. It participates in the synthesis of fucose which serves as a substrate for fucosyltransferases in glycan decorations. The enzymatic activity of GMDS occurs as part of a greater network within the cytosol although it does not directly involve multi-protein complexes. Its product GDP-fucose plays a role in cell-cell interaction and communication emphasizing GMDS’s importance in cell biology and molecular signaling.

Pathways

GMDS contributes to the fucose biosynthesis pathway and indirectly affects the Leloir pathway of galactose metabolism due to interconnected metabolic pools. Through its product GDP-fucose GMDS links to proteins such as fucosyltransferases which incorporate fucose into glycan structures. The availability of fucose also affects signaling pathways involving Notch receptor activation influencing cellular differentiation and growth making GMDS an essential player in glycan-related signal transduction.

GMDS alterations have connections with immunodeficiencies and various cancer types. Deficiencies in GMDS can lead to Congenital Disorder of Glycosylation Type Iic where improper glycosylation affects immune cell functions and fucosylation imbalance links to increased tumor progression and metastasis through altered cell surface glycan structures. In the context of these disorders GMDS influences proteins such as alpha-13-fucosyltransferases with fucose content affecting functional glycan patterns on cells demonstrating its importance in pathophysiology related to glycosylation disorders.
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일반 정보

기능

Catalyzes the conversion of GDP-D-mannose to GDP-4-dehydro-6-deoxy-D-mannose.

서열 유사성

Belongs to the NAD(P)-dependent epimerase/dehydratase family. GDP-mannose 4,6-dehydratase subfamily.

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제품 프로토콜

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타겟 정보

Catalyzes the conversion of GDP-D-mannose to GDP-4-dehydro-6-deoxy-D-mannose.
See full target information GDP-mannose 4,6 dehydratase
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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