Recombinant Human GNAI1 protein (His tag N-Terminus)

일반 정보

기능

Guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades (PubMed : 18434541, PubMed : 33762731, PubMed : 34239069, PubMed : 35610220, PubMed : 37935376, PubMed : 37935377, PubMed : 37963465, PubMed : 38552625, PubMed : 8774883, PubMed : 38918398, PubMed : 40080544). The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state (PubMed : 18434541, PubMed : 8774883). Signaling by an activated GPCR promotes GDP release and GTP binding (PubMed : 18434541, PubMed : 8774883). The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal (PubMed : 18434541, PubMed : 8774883). Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins (PubMed : 18434541, PubMed : 8774883). Signaling is mediated via effector proteins, such as adenylate cyclase : inhibits adenylate cyclase activity of ADCY1, ADCY5 and ADCY6, leading to decreased intracellular cAMP levels (PubMed : 8119955). The inactive GDP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. Required for normal cytokinesis during mitosis (PubMed : 17635935). Required for cortical dynein-dynactin complex recruitment during metaphase (PubMed : 22327364).

서열 유사성

Belongs to the G-alpha family. G(i/o/t/z) subfamily.

Post-translational modifications

Myristoylation at Gly-2 is required for membrane anchoring before palmitoylation.. Palmitoylation at Cys-3 varies with membrane lipid composition.. (Microbial infection) Deamidated at Gln-204 by Photorhabdus asymbiotica toxin PAU_02230, blocking GTP hydrolysis of heterotrimeric GNAQ or GNA11 and G-alphai (GNAI1, GNAI2 or GNAI3) proteins, thereby activating RhoA.