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AB323108

Recombinant Human Granzyme K Protein Standard

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Recombinant Human Granzyme K Protein Standard is a Human Fragment protein, expressed in HEK 293 cells, with >80%, suitable for sELISA, SDS-PAGE.
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Sandwich ELISA - Recombinant Human Granzyme K Protein Standard (AB323108)
  • sELISA

Supplier Data

Sandwich ELISA - Recombinant Human Granzyme K Protein Standard (AB323108)

Sandwich ELISA with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

SDS-PAGE - Recombinant Human Granzyme K Protein Standard (AB323108)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human Granzyme K Protein Standard (AB323108)

SDS-PAGE analysis of ab323108 under reducing conditions for 2ug protein.

주요 정보

Purity

>80% SDS-PAGE

발현 시스템

HEK 293 cells

Tags

Tag free

Applications

sELISA, SDS-PAGE

applications

Biologically active

No

Accession

P49863-1

Animal free

Yes

Carrier free

No

Species

Human

보관 버퍼

pH: 7.3 - 7.5 Constituents: 2.922% Sodium chloride, 0.64107% disodium;hydrogen phosphate;dodecahydrate, 0.02858% Potassium phosphate monobasic

storage-buffer

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보완 제품

Reagents, Controls & Accessories

AB313996

Prestained Protein Ladder - Extra broad molecular weight (3 - 260 kDa)

SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (3 - 260 kDa) (AB313996)

  • 0
  • 0
Proteins & Peptides

AB316642

Recombinant Mouse PR3 Protein Standard (His tag)

Sandwich ELISA - Recombinant Mouse PR3 Protein Standard (His tag) (AB316642)

  • 0
  • 0
Proteins & Peptides

AB99443

Recombinant Human Fibrillarin protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human Fibrillarin protein (His tag N-Terminus) (AB99443)

  • 0
  • 0
Assay Kits

AB314368

Human Granzyme K ELISA Kit

SimpleStep

ELISA - Human Granzyme K ELISA Kit (AB314368)

  • 0
  • 0
Primary Antibodies

AB282703

Anti-Granzyme K antibody [EPR24601-164]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme K antibody [EPR24601-164] (AB282703)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Support team to know which antibody pair is suitable for this protein.
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서열 정보

[{"linker":null,"sequence":null,"proteinLength":"Fragment","predictedMolecularWeight":"26.6 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P49863","tags":[]}]
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification His Tag
배송 시 보관 조건
Dry Ice
적절한 단기 보관 조건
-80°C
적절한 장기 보관 조건
-80°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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일반 정보

기능

Serine protease that initiates the GZMK pathway of the complement system, a cascade of proteins directly activated by CD8(+) T-cells that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system (PubMed : 3262682, PubMed : 39814882, PubMed : 39914456). GZMK is specifically secreted by CD8(+) T-cells and mediates both recognition and initiation steps of GZMK complement pathway (PubMed : 39914456). First acts as a pattern recognition receptor, which specifically recognizes and binds heparan sulfate glycosaminoglycans on the pathogen surface to drive opsonization (PubMed : 39914456). It then initiates the complement pathway cascade by catalyzing cleavage and activation of C2 and C4, the next components of the complement pathway (PubMed : 39814882, PubMed : 39914456). GZMK-mediated complement activation is an important contributor to tissue inflammation (PubMed : 24711407, PubMed : 33271118, PubMed : 35704599, PubMed : 39614076, PubMed : 39814882, PubMed : 39914456). Also able to cleave and activate F2R/PAR1 and PAR2/F2RL1, possibly promoting interleukin release (PubMed : 21760880, PubMed : 26936634, PubMed : 35881628). May also regulate apoptosis by catalyzing cleavage and activation of BID, thereby promoting cytochrome C release from mitochrondria (PubMed : 17308307).

서열 유사성

Belongs to the peptidase S1 family. Granzyme subfamily.

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제품 프로토콜

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타겟 정보

Serine protease that initiates the GZMK pathway of the complement system, a cascade of proteins directly activated by CD8(+) T-cells that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system (PubMed : 3262682, PubMed : 39814882, PubMed : 39914456). GZMK is specifically secreted by CD8(+) T-cells and mediates both recognition and initiation steps of GZMK complement pathway (PubMed : 39914456). First acts as a pattern recognition receptor, which specifically recognizes and binds heparan sulfate glycosaminoglycans on the pathogen surface to drive opsonization (PubMed : 39914456). It then initiates the complement pathway cascade by catalyzing cleavage and activation of C2 and C4, the next components of the complement pathway (PubMed : 39814882, PubMed : 39914456). GZMK-mediated complement activation is an important contributor to tissue inflammation (PubMed : 24711407, PubMed : 33271118, PubMed : 35704599, PubMed : 39614076, PubMed : 39814882, PubMed : 39914456). Also able to cleave and activate F2R/PAR1 and PAR2/F2RL1, possibly promoting interleukin release (PubMed : 21760880, PubMed : 26936634, PubMed : 35881628). May also regulate apoptosis by catalyzing cleavage and activation of BID, thereby promoting cytochrome C release from mitochrondria (PubMed : 17308307).
See full target information GZMK

대체 명칭 보기

TRYP2, GZMK, Granzyme K, GrK, Fragmentin-3, Granzyme-3, NK-tryptase-2, NK-Tryp-2
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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