AB280333

Recombinant human Growth Hormone protein (Active)

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Recombinant human Growth Hormone protein (Active) is a Human Full Length protein, in the 27 to 217 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS, Mass Spec, sELISA, HPLC.

대체 명칭 보기

Somatotropin, Growth hormone, Growth hormone 1, Pituitary growth hormone, GH, GH-N, GH1

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Functional Studies - Recombinant human Growth Hormone protein (Active) (AB280333)

FuncS

Supplier Data

Functional Studies - Recombinant human Growth Hormone protein (Active) (AB280333)

Fully biologically active determined by the dose dependent cell proliferation of rat Nb2-11 lymphoma cell line. ED50 is ≤ 0.21 ng/ml, corresponding to a specific activity of 4.76 x 106 units/mg.

Sandwich ELISA - Recombinant human Growth Hormone protein (Active) (AB280333)

sELISA

Lab

Sandwich ELISA - Recombinant human Growth Hormone protein (Active) (AB280333)

Sandwich ELISA - Recombinant human Growth Hormone protein standard curve.

Background subtracted standard curve using Human Growth Hormone Antibody Pair - BSA and Azide free (ab241146) and Recombinant human Growth Hormone protein (ab280333) in sandwich ELISA. The ELISA was performed using the components of the corresponding SimpleStep® kit, which uses the same antibody pair with a different formulation and format.

Mass Spectrometry - Recombinant human Growth Hormone protein (Active) (AB280333)

Mass Spec

Supplier Data

Mass Spectrometry - Recombinant human Growth Hormone protein (Active) (AB280333)

Mass determination by ESI-TOF.

Predicted MW is 22186.1 Da (+/- 10 Da by ESI-TOF). Observed mass is 22187.15

SDS-PAGE - Recombinant human Growth Hormone protein (Active) (AB280333)

SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human Growth Hormone protein (Active) (AB280333)

SDS-PAGE analysis of ab280333.

HPLC - Recombinant human Growth Hormone protein (Active) (AB280333)

HPLC

Supplier Data

HPLC - Recombinant human Growth Hormone protein (Active) (AB280333)

HPLC analysis of ab280333.

주요 정보

Purity

>95% SDS-PAGE

=95% Purity by HPLC

Endotoxin 레벨

<0.005 EU/µg

발현 시스템

HEK 293 cells

Applications

sELISA, SDS-PAGE, Mass Spec, HPLC, FuncS

applications

Biologically active

Yes

Biological activity

Fully biologically active determined by the dose dependent cell proliferation of rat Nb2-11 lymphoma cell line.

ED50 is ≤ 0.21 ng/ml, corresponding to a specific activity of 4.76 x 106 units/mg.

Accession

Animal free

Yes

Carrier free

Species

Human

Reconstitution

Reconstitute in PBS

보관 버퍼

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" } } }

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서열 정보

[{"linker":null,"sequence":"FPTIPLSRLFDNAMLRAHRLHQLAFDTYQEFEEAYIPKEQKYSFLQNPQTSLCFSESIPTPSNREETQQKSNLELLRISLLLIQSWLEPVQFLRSVFANSLVYGASDSNVYDLLKDLEEGIQTLMGRLEDGSPRTGQIFKQTYSKFDTNSHNDDALLKNYGLLYCFRKDMDKVETFLRIVQCRSVEGSCGF","proteinLength":"Full Length","predictedMolecularWeight":"22.19 kDa","actualMolecularWeight":"22.19 kDa","aminoAcidEnd":217,"aminoAcidStart":27,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P01241","tags":[]}]

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특성 및 보관 정보

제형

Lyophilized

배송 시 보관 조건

Ambient - Can Ship with Ice

적절한 단기 보관 조건

Ambient

적절한 장기 보관 조건

Ambient

True

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Growth Hormone (GH) also known as somatotropin is a polypeptide hormone with a mass of approximately 22 kDa. The pituitary gland synthesizes and secretes GH. Once synthesized GH circulates in the bloodstream and affects various tissues throughout the body. GH's expression is tightly regulated because of its significant roles in growth processes. Researchers might use anti-growth hormone antibodies and various hGH testing kits including ELISA to study its levels and functions.

Biological function summary

GH triggers growth and cell reproduction by exerting effects on multiple tissues. It binds to the growth hormone receptor which initiates a cascade of reactions that ultimately stimulates growth in muscle bone and cartilage. GH also contributes to protein synthesis and helps regulate metabolism. GH acts mostly independently but can interact with cytokines and other hormones.

Pathways

GH plays important roles in the regulation of the JAK-STAT signaling pathway and the IGF-1 signaling pathway. The JAK-STAT pathway is important for transmitting the signal of GH binding to its receptor into cellular responses. GH stimulates IGF-1 production in the liver and IGF-1 then acts in an endocrine manner to mediate much of GH’s growth-promoting activity. These interactions highlight the interconnectedness of GH with other hormones in growth signaling networks.

Several health conditions can arise from abnormal levels of GH. Dwarfism occurs due to GH deficiency while acromegaly results from GH excess. Clinical analysis might use hGH kits to measure hormone levels and aid in diagnosis. GH's role in such conditions links it to other hormones like insulin and glucagon that help regulate glucose levels further affecting metabolic processes. Recombinant forms of GH are used in therapy for these disorders providing avenues for treatment and management.

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일반 정보

기능

Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.

서열 유사성

Belongs to the somatotropin/prolactin family.

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제품 프로토콜

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

See full target information GH1

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Recombinant human Growth Hormone protein (Active)

Functional Studies - Recombinant human Growth Hormone protein (Active) (AB280333)

Sandwich ELISA - Recombinant human Growth Hormone protein (Active) (AB280333)

Mass Spectrometry - Recombinant human Growth Hormone protein (Active) (AB280333)

SDS-PAGE - Recombinant human Growth Hormone protein (Active) (AB280333)

HPLC - Recombinant human Growth Hormone protein (Active) (AB280333)

주요 정보

Purity

Endotoxin 레벨

발현 시스템

Tags

Applications

Biologically active

Biological activity

Accession

Animal free

Carrier free

Species

Reconstitution

보관 버퍼

보완 제품

Reagents, Controls & Accessories

Proteins & Peptides

Proteins & Peptides

Proteins & Peptides

Primary Antibodies

Reactivity 정보

서열 정보

특성 및 보관 정보

제형

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

추가 정보

Biological function summary

Pathways

일반 정보

기능

서열 유사성

제품 프로토콜

타겟 정보

Product promise