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AB268747

Recombinant Human GWL protein (GST tag N-Terminus)

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Recombinant Human GWL protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 879 aa range, expressed in Baculovirus infected Sf9 cells, with >70%, suitable for SDS-PAGE.
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SDS-PAGE - Recombinant Human GWL protein (GST tag N-Terminus) (AB268747)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human GWL protein (GST tag N-Terminus) (AB268747)

SDS-PAGE analysis of ab268747.

주요 정보

Purity

>70% SDS-PAGE

Affinity purified.

발현 시스템

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q96GX5-1

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

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보완 제품

Primary Antibodies

AB86387

Anti-GWL antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GWL antibody (AB86387)

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Proteins & Peptides

AB276667

Recombinant Human FAM150A protein (Fc Chimera)

SDS-PAGE - Recombinant Human FAM150A protein (Fc Chimera) (AB276667)

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Assay Kits

AB214568

Human IgM ELISA Kit

SimpleStep

ELISA - Human IgM ELISA Kit (AB214568)

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Proteins & Peptides

AB202191

Recombinant Human PCOLCE protein (denatured) (His tag N-Terminus)

SDS-PAGE - Recombinant Human PCOLCE protein (denatured) (His tag N-Terminus) (AB202191)

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  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"MDPTAGSKKEPGGGAATEEGVNRIAVPKPPSIEEFSIVKPISRGAFGKVYLGQKGGKLYAVKVVKKADMINKNMTHQVQAERDALALSKSPFIVHLYYSLQSANNVYLVMEYLIGGDVKSLLHIYGYFDEEMAVKYISEVALALDYLHRHGIIHRDLKPDNMLISNEGHIKLTDFGLSKVTLNRDINMMDILTTPSMAKPRQDYSRTPGQVLSLISSLGFNTPIAEKNQDPANILSACLSETSQLSQGLVCPMSVDQKDTTPYSSKLLKSCLETVASNPGMPVKCLTSNLLQSRKRLATSSASSQSHTFISSVESECHSSPKWEKDCQESDEALGPTMMSWNAVEKLCAKSANAIETKGFNKKDLELALSPIHNSSALPTTGRSCVNLAKKCFSGEVSWEAVELDVNNINMDTDTSQLGFHQSNQWAVDSGGISEEHLGKRSLKRNFELVDSSPCKKIIQNKKTCVEYKHNEMTNCYTNQNTGLTVEVQDLKLSVHKSQQNDCANKENIVNSFTDKQQTPEKLPIPMIAKNLMCELDEDCEKNSKRDYLSSSFLCSDDDRASKNISMNSDSSFPGISIMESPLESQPLDSDRSIKESSFEESNIEDPLIVTPDCQEKTSPKGVENPAVQESNQKMLGPPLEVLKTLASKRNAVAFRSFNSHINASNNSEPSRMNMTSLDAMDISCAYSGSYPMAITPTQKRRSCMPHQQTPNQIKSGTPYRTPKSVRRGVAPVDDGRILGTPDYLAPELLLGRAHGPAVDWWALGVCLFEFLTGIPPFNDETPQQVFQNILKRDIPWPEGEEKLSDNAQSAVEILLTIDDTKRAGMKELKRHPLFSDVDWENLQHQTMPFIPQPDDETDTSYFEARNTAQHLTVSGFSL","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":879,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q96GX5","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification
배송 시 보관 조건
Dry Ice
적절한 단기 보관 조건
-80°C
적절한 장기 보관 조건
-80°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

GWL also known as Greatwall kinase (and occasionally Mastl) is a protein kinase with a calculated molecular mass of about 58 kDa. This protein mainly expresses in various tissues but it shows higher expression in organs such as the heart liver and skeletal muscle. GWL plays a mechanical role in the regulation of cell cycle through its kinase activity which phosphorylates other key proteins involved in mitosis.
Biological function summary

GWL influences the cell cycle and mitotic progression by modulating the activity of the phosphatase inhibitor proteins ENSA and ARPP19. It is part of a functional complex with these proteins when activated leading to the inhibition of Protein Phosphatase 2A (PP2A) during mitosis which allows for proper chromosome condensation and segregation. This ensures that the cells divide accurately maintaining genomic stability.

Pathways

Different cellular pathways integrate their signals through GWL to regulate mitosis. One of the key pathways is the cell cycle control pathway where GWL works alongside CDK1 and is essential for the phosphorylation cascade that leads to mitotic entry. Another pathway of note is the mitotic spindle assembly checkpoint where GWL interacts with proteins such as BUB1 and MAD2 helping establish mechanisms that ensure cells do not prematurely progress through mitosis with errors.

GWL dysfunction links to cancer due to its critical role in cell division. Aberrant expression or mutation of GWL can lead to aneuploidy and genomic instability factors that contribute to tumorigenesis. Moreover abnormalities in GWL expression have been associated with neurological disorders like Alzheimer's disease where it may interact with tau protein aggregation pathways. In cancer GWL often works in conjunction with oncogenes like MYC and tumor suppressors such as p53 influencing their roles in controlling cell proliferation and survival.
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일반 정보

기능

Serine/threonine kinase that plays a key role in M phase by acting as a regulator of mitosis entry and maintenance (PubMed : 19680222). Acts by promoting the inactivation of protein phosphatase 2A (PP2A) during M phase : does not directly inhibit PP2A but acts by mediating phosphorylation and subsequent activation of ARPP19 and ENSA at 'Ser-62' and 'Ser-67', respectively (PubMed : 38123684). ARPP19 and ENSA are phosphatase inhibitors that specifically inhibit the PPP2R2D (PR55-delta) subunit of PP2A. Inactivation of PP2A during M phase is essential to keep cyclin-B1-CDK1 activity high (PubMed : 20818157). Following DNA damage, it is also involved in checkpoint recovery by being inhibited. Phosphorylates histone protein in vitro; however such activity is unsure in vivo. May be involved in megakaryocyte differentiation.

서열 유사성

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.

Post-translational modifications

Phosphorylation at Thr-741 by CDK1 during M phase activates its kinase activity (By similarity). Maximum phosphorylation occurs in prometaphase.

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제품 프로토콜

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타겟 정보

Serine/threonine kinase that plays a key role in M phase by acting as a regulator of mitosis entry and maintenance (PubMed : 19680222). Acts by promoting the inactivation of protein phosphatase 2A (PP2A) during M phase : does not directly inhibit PP2A but acts by mediating phosphorylation and subsequent activation of ARPP19 and ENSA at 'Ser-62' and 'Ser-67', respectively (PubMed : 38123684). ARPP19 and ENSA are phosphatase inhibitors that specifically inhibit the PPP2R2D (PR55-delta) subunit of PP2A. Inactivation of PP2A during M phase is essential to keep cyclin-B1-CDK1 activity high (PubMed : 20818157). Following DNA damage, it is also involved in checkpoint recovery by being inhibited. Phosphorylates histone protein in vitro; however such activity is unsure in vivo. May be involved in megakaryocyte differentiation.
See full target information MASTL

대체 명칭 보기

GW, GWL, THC2, MASTL, Serine/threonine-protein kinase greatwall, hGWL, Microtubule-associated serine/threonine-protein kinase-like, MAST-L
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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