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AB241318

Recombinant human HAO1/GOX protein (Active) (His tag N-Terminus)

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Recombinant human HAO1/GOX protein (Active) (His tag N-Terminus) is a Human Full Length protein, in the 1 to 370 aa range, expressed in Escherichia coli, with >95%, < 0.1 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS, Mass Spec.

대체 명칭 보기

GOX1, HAO1, 2-Hydroxyacid oxidase 1, HAOX1, Glycolate oxidase, Glyoxylate oxidase, GO, GOX

1 이미지
SDS-PAGE - Recombinant human HAO1/GOX protein (Active) (His tag N-Terminus) (AB241318)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human HAO1/GOX protein (Active) (His tag N-Terminus) (AB241318)

15% SDS-PAGE analysis of 3 μg ab241318.

주요 정보

Purity

>95% SDS-PAGE

Purified using conventional chromatography.

Endotoxin 레벨

< 0.1 EU/µg

발현 시스템

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Specific activity is > 3,000 pmol/min/μg, and defined as the amount of enzyme that oxidize glyoxylate at pH 8.0 at 25°C.

Accession

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 2.9% Sodium chloride, 0.32% Tris HCl

storage-buffer

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보완 제품

Reagents, Controls & Accessories

AB133411

Low Fluorescence Western Membrane (PVDF)

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Proteins & Peptides

AB239568

Recombinant Human HAO2 protein (His tag)

SDS-PAGE - Recombinant Human HAO2 protein (His tag) (AB239568)

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Biochemicals

AB146349

Riboflavin-5-phosphate sodium salt dihydrate, Enzyme cofactor

Chemical Structure - Riboflavin-5-phosphate sodium salt dihydrate, Enzyme cofactor (AB146349)

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Assay Kits

AB309417

Human HAO1/GOX ELISA Kit

Sandwich ELISA - Human HAO1/GOX ELISA Kit (AB309417)

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Primary Antibodies

AB194790

Anti-HAO1/GOX antibody

Western blot - Anti-HAO1/GOX antibody (AB194790)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSMLPRLICINDYEQHAKSVLPKSIYDYYRSGANDEETLADNIAAFSRWKLYPRMLRNVAETDLSTSVLGQRVSMPICVGATAMQRMAHVDGELATVRACQSLGTGMMLSSWATSSIEEVAEAGPEALRWLQLYIYKDREVTKKLVRQAEKMGYKAIFVTVDTPYLGNRLDDVRNRFKLPPQLRMKNFETSTLSFSPEENFGDDSGLAAYVAKAIDPSISWEDIKWLRRLTSLPIVAKGILRGDDAREAVKHGLNGILVSNHGARQLDGVPATIDVLPEIVEAVEGKVEVFLDGGVRKGTDVLKALALGAKAVFVGRPIVWGLAFQGEKGVQDVLEILKEEFRLAMALSGCQNVKVIDKTLVRKNPLAVSKI","proteinLength":"Full Length","predictedMolecularWeight":"45 kDa","actualMolecularWeight":null,"aminoAcidEnd":370,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9UJM8","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
True
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The enzyme HAO1 also known as GOX (glycolate oxidase) functions by catalyzing the oxidation of glycolate to glyoxylate generating hydrogen peroxide in peroxisomes. The molecular mass of HAO1 is approximately 43 kDa. It is expressed in several tissues with high levels found in liver and kidney. The protein may also be referred to as alpha GOX which highlights its identity and specialization within oxidase family.
Biological function summary

HAO1 facilitates the metabolism of hydroxyl acids playing an important role in glyoxylate and dicarboxylate metabolism. It does not form part of a multi-protein complex but operates as a monomer maintaining cellular homeostasis of organic acids. By helping convert glycolate into a form that cells use or excrete HAO1 prevents the accumulation of compounds that can become toxic at elevated levels.

Pathways

Glycolate oxidase takes part in the glyoxylate cycle and photorespiration in plants which also has parallels in animal metabolism where it participates in handling waste products. HAO1 interacts within the network of metabolic pathways along with proteins like lactate dehydrogenase that facilitate the conversion of chemical compounds. It plays a role in broader metabolomic balancing acts that maintain overall organismal homeostasis.

Mutations and dysfunctions in HAO1 have links to primary hyperoxaluria type 1 a disease characterized by excessive production and build-up of oxalate leading to kidney stones and renal failure. The interplay with proteins like alanine:glyoxylate aminotransferase which helps detoxify glyoxylate is important in these conditions. This disorder highlights the importance of proper glycolate metabolism and its regulation in maintaining health.
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일반 정보

기능

Broad substrate specificity (S)-2-hydroxy-acid oxidase that preferentially oxidizes glycolate (PubMed : 10777549, PubMed : 10978532, PubMed : 17669354, PubMed : 18215067). The glyoxylate produced by the oxidation of glycolate can then be utilized by alanine-glyoxylate aminotransferase for the peroxisomal synthesis of glycine; this pathway appears to be an important step for the detoxification of glyoxylate which, if allowed to accumulate, may be metabolized to oxalate with formation of kidney stones (PubMed : 10978532, PubMed : 17669354). Can also catalyze the oxidation of glyoxylate, and long chain hydroxyacids such as 2-hydroxyhexadecanoate and 2-hydroxyoctanoate, albeit with much lower catalytic efficiency (PubMed : 10777549, PubMed : 17669354, PubMed : 18215067). Active in vitro with the artificial electron acceptor 2,6-dichlorophenolindophenol (DCIP), but O2 is believed to be the physiological electron acceptor, leading to the production of H2O2 (PubMed : 10777549, PubMed : 10978532, PubMed : 17669354, PubMed : 18215067). Is not active on L-lactate and 2-hydroxybutanoate (PubMed : 10777549).

서열 유사성

Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.

Subcellular localisation

Peroxisome matrix

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제품 프로토콜

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타겟 정보

Broad substrate specificity (S)-2-hydroxy-acid oxidase that preferentially oxidizes glycolate (PubMed : 10777549, PubMed : 10978532, PubMed : 17669354, PubMed : 18215067). The glyoxylate produced by the oxidation of glycolate can then be utilized by alanine-glyoxylate aminotransferase for the peroxisomal synthesis of glycine; this pathway appears to be an important step for the detoxification of glyoxylate which, if allowed to accumulate, may be metabolized to oxalate with formation of kidney stones (PubMed : 10978532, PubMed : 17669354). Can also catalyze the oxidation of glyoxylate, and long chain hydroxyacids such as 2-hydroxyhexadecanoate and 2-hydroxyoctanoate, albeit with much lower catalytic efficiency (PubMed : 10777549, PubMed : 17669354, PubMed : 18215067). Active in vitro with the artificial electron acceptor 2,6-dichlorophenolindophenol (DCIP), but O2 is believed to be the physiological electron acceptor, leading to the production of H2O2 (PubMed : 10777549, PubMed : 10978532, PubMed : 17669354, PubMed : 18215067). Is not active on L-lactate and 2-hydroxybutanoate (PubMed : 10777549).
See full target information HAO1
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Product promise

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