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AB42633

Recombinant Human HDAC8 protein (His tag C-Terminus)

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Recombinant Human HDAC8 protein (His tag C-Terminus) is a Human Full Length protein, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for FuncS (Neut/Block).

주요 정보

Purity

>90% SDS-PAGE

Affinity purified.

발현 시스템

Baculovirus infected Sf9 cells

Tags

His tag C-Terminus

Applications

FuncS (Neut/Block)

applications

Biologically active

No

Accession

Q9BY41-1

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.8004% Sodium chloride, 0.79% Tris HCl

storage-buffer

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보완 제품

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "FuncS (Neut/Block)": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Expressed in a Baculovirus infected Sf9 cell expression system. MW= 46.4 kDa. 10 U/ug. One U =1 pmol/min, Assay condition: 25 mM Tris/Cl, pH8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 100 uM Biomol substrate (Catalog number KI-178), and 15 ng/ul HDAC8. Incubation condition: 60 min at 30ºC.
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서열 정보

[{"linker":null,"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q9BY41","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification
배송 시 보관 조건
Dry Ice
적절한 단기 보관 조건
-80°C
적절한 장기 보관 조건
-80°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The protein HDAC8 also known as Histone Deacetylase 8 functions mechanically by removing acetyl groups from histone tails influencing chromatin structure and gene expression. HDAC8 has a molecular mass of roughly 41 kDa. It expresses in various tissues but shows higher expression in smooth muscle cells and certain cancer cell lines. Researchers also note its expression in the brain and liver corresponding to sections that require its deacetylase activity for various regulatory functions.
Biological function summary

HDAC8 plays a role in regulating transcription by being involved in altering chromatin structure. It does not form part of a large multi-protein complex unlike some other histone deacetylases. Its activity affects cell cycle progression and differentiation which is central to cellular physiology. HDAC8 also participates in non-histone protein deacetylation impacting various substrates allowing it to govern diverse cellular processes.

Pathways

Scientists associate HDAC8 with cell cycle regulation and muscle contraction. HDAC8 plays a role in the molecular pathways that control how myosin light chains undergo deacetylation interacting with proteins such as smooth muscle actin. During cell cycle regulation HDAC8 affects p53 a protein critical in controlling cell cycle progression and apoptosis integrating into pathways that decide cellular outcomes during division and response to stress.

Researchers identify HDAC8 as having connections to certain cancers and Cornelia de Lange Syndrome (CdLS). Its deregulation links to tumorigenesis particularly in colorectal and neuroblastoma cancers where it may interact with oncogenes and tumor suppressors. In CdLS mutations that affect HDAC8 lead to developmental issues with the disorder being connected to the cohesin complex proteins which highlight HDAC8's importance in chromosomal cohesion and gene expression regulation.
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일반 정보

기능

Histone deacetylase that catalyzes the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) (PubMed : 10748112, PubMed : 10922473, PubMed : 10926844, PubMed : 14701748, PubMed : 28497810). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events (PubMed : 10748112, PubMed : 10922473, PubMed : 10926844, PubMed : 14701748). Histone deacetylases act via the formation of large multiprotein complexes (PubMed : 10748112, PubMed : 10922473, PubMed : 10926844, PubMed : 14701748). Also involved in the deacetylation of cohesin complex protein SMC3 regulating release of cohesin complexes from chromatin (PubMed : 22885700). May play a role in smooth muscle cell contractility (PubMed : 15772115). In addition to protein deacetylase activity, also has protein-lysine deacylase activity : acts as a protein decrotonylase by mediating decrotonylation ((2E)-butenoyl) of histones (PubMed : 28497810).

서열 유사성

Belongs to the histone deacetylase family. HD type 1 subfamily.

Post-translational modifications

Phosphorylated by PKA on serine 39. Phosphorylation reduces deacetylase activity observed preferentially on histones H3 and H4.

Subcellular localisation

Nucleus

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제품 프로토콜

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타겟 정보

Histone deacetylase that catalyzes the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) (PubMed : 10748112, PubMed : 10922473, PubMed : 10926844, PubMed : 14701748, PubMed : 28497810). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events (PubMed : 10748112, PubMed : 10922473, PubMed : 10926844, PubMed : 14701748). Histone deacetylases act via the formation of large multiprotein complexes (PubMed : 10748112, PubMed : 10922473, PubMed : 10926844, PubMed : 14701748). Also involved in the deacetylation of cohesin complex protein SMC3 regulating release of cohesin complexes from chromatin (PubMed : 22885700). May play a role in smooth muscle cell contractility (PubMed : 15772115). In addition to protein deacetylase activity, also has protein-lysine deacylase activity : acts as a protein decrotonylase by mediating decrotonylation ((2E)-butenoyl) of histones (PubMed : 28497810).
See full target information HDAC8

대체 명칭 보기

HDACL1, CDA07, HDAC8, Histone deacetylase 8, HD8, Protein deacetylase HDAC8, Protein decrotonylase HDAC8
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Product promise

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