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AB124585

Recombinant Human HIBCH protein (His tag N-Terminus)

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Recombinant Human HIBCH protein (His tag N-Terminus) is a Human Full Length protein, in the 33 to 386 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

대체 명칭 보기

3-hydroxyisobutyryl-coenzyme A hydrolase, HIB-CoA hydrolase, HIBYL-CoA-H, HIBCH

1 이미지
SDS-PAGE - Recombinant Human HIBCH protein (His tag N-Terminus) (AB124585)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human HIBCH protein (His tag N-Terminus) (AB124585)

15% SDS-PAGE showing ab124585 at approximately 42.1kDa (3μg).

주요 정보

Purity

>90% SDS-PAGE

ab124585 is purified using conventional chromatography techniques.

발현 시스템

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 1.17% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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보완 제품

Biochemicals

AB146200

2-NBD-Glucose, Fluorescent glucose uptake probe

Chemical Structure - 2-NBD-Glucose, Fluorescent glucose uptake probe (AB146200)

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Biochemicals

AB146373

DTNB, sulfhydryl reagent

Chemical Structure - DTNB, sulfhydryl reagent (AB146373)

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Biochemicals

AB146313

Ponceau S, Protein dye

Chemical Structure - Ponceau S, Protein dye (AB146313)

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Primary Antibodies

AB153826

Anti-HIBCH antibody

Immunocytochemistry/ Immunofluorescence - Anti-HIBCH antibody (AB153826)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"MGSSHHHHHHSSGLVPRGSHMGSHMDAAEEVLLEKKGCTGVITLNRPKFLNALTLNMIRQIYPQLKKWEQDPETFLIIIKGAGGKAFCAGGDIRVISEAEKAKQKIAPVFFREEYMLNNAVGSCQKPYVALIHGITMGGGVGLSVHGQFRVATEKCLFAMPETAIGLFPDVGGGYFLPRLQGKLGYFLALTGFRLKGRDVYRAGIATHFVDSEKLAMLEEDLLALKSPSKENIASVLENYHTESKIDRDKSFILEEHMDKINSCFSANTVEEIIENLQQDGSSFALEQLKVINKMSPTSLKITLRQLMEGSSKTLQEVLTMEYRLSQACMRGHDFHEGVRAVLIDKDQSPKWKPADLKEVTEEDLNNHFKSLGSSDLKF","proteinLength":"Full Length","predictedMolecularWeight":"42.1 kDa","actualMolecularWeight":null,"aminoAcidEnd":386,"aminoAcidStart":33,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q6NVY1","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

HIBCH or 3-hydroxyisobutyryl-CoA hydrolase is an enzyme involved in the catabolic pathway of valine metabolism. It exhibits a molecular mass of approximately 37 kDa. This enzyme processes 3-hydroxyisobutyryl-CoA into free 3-hydroxyisobutyrate and CoA. HIBCH is expressed in several tissues with high levels found in liver and kidney reflecting its active role in amino acid metabolism.
Biological function summary

The enzyme catalyzes a specific reaction in the branched-chain amino acid metabolic pathway which is an essential process for the degradation and utilization of valine. HIBCH functions independently not forming part of any larger complex. This enzyme is key for maintaining the balance of metabolites derived from valine and is involved in energy production from protein breakdown.

Pathways

HIBCH plays a significant role in the valine degradation pathway where it acts downstream of other enzymes like acyl-CoA dehydrogenase. During this pathway it collaborates with various enzymes to ensure efficient energy extraction from valine. This process links valine metabolism to the larger citric acid cycle where it coordinates with enzymes such as isovaleryl-CoA dehydrogenase.

Mutations or deficiencies in HIBCH correlate with 3-hydroxyisobutyryl-CoA hydrolase deficiency a metabolic disorder that can manifest as developmental delay neurological deficits or acute metabolic crises in severe cases. The enzyme has links to valine metabolism-related disorders where disturbances in its function can lead to elevated levels of neurotoxic intermediates. Its interaction with related enzymes such as branched-chain alpha-keto acid dehydrogenase further accentuates its importance in maintaining metabolic equilibrium.
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일반 정보

기능

Hydrolyzes 3-hydroxyisobutyryl-CoA (HIBYL-CoA), a saline catabolite. Has high activity toward isobutyryl-CoA. Could be an isobutyryl-CoA dehydrogenase that functions in valine catabolism. Also hydrolyzes 3-hydroxypropanoyl-CoA.

서열 유사성

Belongs to the enoyl-CoA hydratase/isomerase family.

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제품 프로토콜

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타겟 정보

Hydrolyzes 3-hydroxyisobutyryl-CoA (HIBYL-CoA), a saline catabolite. Has high activity toward isobutyryl-CoA. Could be an isobutyryl-CoA dehydrogenase that functions in valine catabolism. Also hydrolyzes 3-hydroxypropanoyl-CoA.
See full target information HIBCH
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Product promise

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