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AB109927

Recombinant Human HMGA1a / HMGA1b protein (His tag C-Terminus)

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Recombinant Human HMGA1a / HMGA1b protein (His tag C-Terminus) is a Human Full Length protein, in the 1 to 107 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

대체 명칭 보기

HMGIY, HMGA1, High mobility group protein HMG-I/HMG-Y, HMG-I(Y), High mobility group AT-hook protein 1, High mobility group protein R, High mobility group protein A1

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SDS-PAGE - Recombinant Human HMGA1a / HMGA1b protein (His tag C-Terminus) (AB109927)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human HMGA1a / HMGA1b protein (His tag C-Terminus) (AB109927)

15% SDS-PAGE showing ab109927 at approximately 12.7 kDa (3µg).

주요 정보

Purity

>90% SDS-PAGE

ab109927 is purified using conventional chromatography techniques.

발현 시스템

Escherichia coli

Tags

His tag C-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 8 Constituents: 50% Glycerol (glycerin, glycerine), 1.12% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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보완 제품

Primary Antibodies

AB129153

Anti-HMGA1 antibody [EPR7839]

20ul selling size|Recombinant|Advanced Validation|RabMAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA1 antibody [EPR7839] (AB129153)

  • 5
  • 3
Cell Lines & Lysates

AB261873

Human HIST1H1C (Histone H1.2) knockout A549 cell line

Advanced Validation

Western blot - Human HIST1H1C (Histone H1.2) knockout A549 cell line (AB261873)

  • 0
  • 0
Primary Antibodies

AB300437

Anti-CHD5 antibody [EPR23858-3-8]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHD5 antibody [EPR23858-3-8] (AB300437)

  • 0
  • 0
Primary Antibodies

AB214821

Anti-BMP2 antibody [EPR20807]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-BMP2 antibody [EPR20807] (AB214821)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"MSESSSKSSQPLASKQEKDGTEKRGRGRPRKQPPVSPGTALVGSQKEPSEVPTPKRPRGRPKGSKNKGAAKTRKTTTTPGRKPRGRPKKLEKEEEEGISQESSEEEQLEHHHHHH","proteinLength":"Full Length","predictedMolecularWeight":"12.7 kDa","actualMolecularWeight":null,"aminoAcidEnd":107,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P17096","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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일반 정보

기능

HMG-I/Y bind preferentially to the minor groove of A+T rich regions in double-stranded DNA. It is suggested that these proteins could function in nucleosome phasing and in the 3'-end processing of mRNA transcripts. They are also involved in the transcription regulation of genes containing, or in close proximity to A+T-rich regions.

서열 유사성

Belongs to the HMGA family.

Post-translational modifications

Constitutively phosphorylated on two or three sites. Hyperphosphorylated at early stages of apoptosis, followed by dephosphorylation and methylation, which coincides with chromatin condensation. Isoforms HMG-I and HMG-Y can be phosphorylated by HIPK2. Phosphorylation of HMG-I at Ser-36, Thr-53 and Thr-78 and of HMG-Y at Thr-42 and Thr-67 by HIPK2 modulates DNA-binding affinity.. HMG-Y is not methylated.. Methylation at Arg-58 is mutually exclusive with methylation at Arg-60.

Subcellular localisation

Nucleus

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제품 프로토콜

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타겟 정보

HMG-I/Y bind preferentially to the minor groove of A+T rich regions in double-stranded DNA. It is suggested that these proteins could function in nucleosome phasing and in the 3'-end processing of mRNA transcripts. They are also involved in the transcription regulation of genes containing, or in close proximity to A+T-rich regions.
See full target information HMGA1
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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