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AB323137

Recombinant Human HMGN1 Protein Standard

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Recombinant Human HMGN1 Protein Standard is a Human Full Length protein, expressed in HEK 293 cells, with >80%, suitable for sELISA, SDS-PAGE.
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Sandwich ELISA - Recombinant Human HMGN1 Protein Standard (AB323137)
  • sELISA

Supplier Data

Sandwich ELISA - Recombinant Human HMGN1 Protein Standard (AB323137)

Sandwich ELISA with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

SDS-PAGE - Recombinant Human HMGN1 Protein Standard (AB323137)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human HMGN1 Protein Standard (AB323137)

SDS-PAGE analysis of ab323137 under reducing conditions for 2ug protein.

주요 정보

Purity

>80% SDS-PAGE

발현 시스템

HEK 293 cells

Tags

Tag free

Applications

SDS-PAGE, sELISA

applications

Biologically active

No

Accession

P05114-1

Animal free

Yes

Carrier free

No

Species

Human

보관 버퍼

pH: 7.3 - 7.5 Constituents: 2.922% Sodium chloride, 0.64107% disodium;hydrogen phosphate;dodecahydrate, 0.02858% Potassium phosphate monobasic

storage-buffer

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보완 제품

Reagents, Controls & Accessories

AB313996

Prestained Protein Ladder - Extra broad molecular weight (3 - 260 kDa)

SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (3 - 260 kDa) (AB313996)

  • 0
  • 0
Primary Antibodies

AB315889

Anti-HMGN1 antibody [EPR27314-67]

20ul selling size|BOND RX™ Validated|Recombinant|RabMAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGN1 antibody [EPR27314-67] (AB315889)

  • 0
  • 0
Primary Antibodies

AB181377

Anti-53BP2/ASPP2/BBP antibody [EPR13837]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP2/ASPP2/BBP antibody [EPR13837] (AB181377)

  • 0
  • 0
Primary Antibodies

AB197845

Anti-K Cadherin/CDH6 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-K Cadherin/CDH6 antibody (AB197845)

  • 4
  • 2
Primary Antibodies

AB181142

Anti-PCSK9 antibody [EPR7627(2)]

20ul selling size|RabMAb|Recombinant

Immunoprecipitation - Anti-PCSK9 antibody [EPR7627(2)] (AB181142)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Support team to know which antibody pair is suitable for this protein.
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서열 정보

[{"linker":null,"sequence":null,"proteinLength":"Full Length","predictedMolecularWeight":"11 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P05114","tags":[]}]
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification His Tag
배송 시 보관 조건
Dry Ice
적절한 단기 보관 조건
-80°C
적절한 장기 보관 조건
-80°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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일반 정보

기능

Binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. May be involved in the process which maintains transcribable genes in a unique chromatin conformation. Inhibits the phosphorylation of nucleosomal histones H3 and H2A by RPS6KA5/MSK1 and RPS6KA3/RSK2 (By similarity).

서열 유사성

Belongs to the HMGN family.

Post-translational modifications

Phosphorylation on Ser-21 and Ser-25 weakens binding to nucleosomes and increases the rate of H3 phosphorylation (By similarity). Phosphorylation favors cytoplasmic localization.

Subcellular localisation

Nucleus

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제품 프로토콜

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타겟 정보

Binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. May be involved in the process which maintains transcribable genes in a unique chromatin conformation. Inhibits the phosphorylation of nucleosomal histones H3 and H2A by RPS6KA5/MSK1 and RPS6KA3/RSK2 (By similarity).
See full target information HMGN1

대체 명칭 보기

HMG14, Non-histone chromosomal protein HMG-14, High mobility group nucleosome-binding domain-containing protein 1
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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