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AB114680

Recombinant Human hnRNP F protein (GST tag N-Terminus)

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Recombinant Human hnRNP F protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 415 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

대체 명칭 보기

HNRPF, HNRNPF, Heterogeneous nuclear ribonucleoprotein F, hnRNP F, Nucleolin-like protein mcs94-1

1 이미지
SDS-PAGE - Recombinant Human hnRNP F protein (GST tag N-Terminus) (AB114680)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human hnRNP F protein (GST tag N-Terminus) (AB114680)

ab114680 analysed on a 12.5% SDS-PAGE gel stained with Coomassie Blue.

주요 정보

발현 시스템

Wheat germ

Tags

GST tag N-Terminus

Applications

ELISA, WB, SDS-PAGE

applications

Biologically active

No

Accession

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 8 Constituents: 0.79% Tris HCl, 0.3% Glutathione

storage-buffer

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보완 제품

Primary Antibodies

AB10689

Anti-hnRNP F+H antibody [1G11]

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP F+H antibody [1G11] (AB10689)

  • 5
  • 9
Proteins & Peptides

AB105612

Recombinant Human Nucleoside-diphosphate kinase protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human Nucleoside-diphosphate kinase protein (His tag N-Terminus) (AB105612)

  • 0
  • 0
Primary Antibodies

AB155103

Anti-Ran antibody [EPR10791(B)]

RabMAb|Recombinant|20ul selling size

Flow Cytometry (Intracellular) - Anti-Ran antibody [EPR10791(B)] (AB155103)

  • 0
  • 0
Primary Antibodies

AB187652

Anti-MCAK antibody [EPR14838]

20ul selling size|RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-MCAK antibody [EPR14838] (AB187652)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"MMLGPEGGEGFVVKLRGLPWSCSVEDVQNFLSDCTIHDGAAGVHFIYTREGRQSGEAFVELGSEDDVKMALKKDRESMGHRYIEVFKSHRTEMDWVLKHSGPNSADSANDGFVRLRGLPFGCTKEEIVQFFSGLEIVPNGITLPVDPEGKITGEAFVQFASQELAEKALGKHKERIGHRYIEVFKSSQEEVRSYSDPPLKFMSVQRPGPYDRPGTARRYIGIVKQAGLERMRPGAYSTGYGGYEEYSGLSDGYGFTTDLFGRDLSYCLSGMYDHRYGDSEFTVQSTTGHCVHMRGLPYKATENDIYNFFSPLNPVRVHIEIGPDGRVTGEADVEFATHEEAVAAMSKDRANMQHRYIELFLNSTTGASNGAYSSQVMQGMGVSAAQATYSGLESQSVSGCYGAGYSGQNSMGGYD","proteinLength":"Full Length","predictedMolecularWeight":"71.72 kDa","actualMolecularWeight":null,"aminoAcidEnd":415,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P52597","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Proprietary technique
배송 시 보관 조건
Dry Ice
적절한 단기 보관 조건
-80°C
적절한 장기 보관 조건
-80°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Heterogeneous nuclear ribonucleoprotein F (hnRNP F) also known as hnRNP-F or HNRNPF plays an important role in RNA processing within the nucleus. It belongs to the family of hnRNPs which bind to nascent pre-mRNA molecules influencing their stability and transport. HnRNP F has a molecular weight of approximately 46 kDa. It is ubiquitously expressed found in various tissues but it exhibits higher levels of expression in the testis and brain. Its structured domains allow it to bind to RNA with high specificity impacting several RNA metabolic processes directly.
Biological function summary

HnRNP F interacts with RNA molecules to modulate alternative splicing mRNA stability and transport. It often forms part of larger ribonucleoprotein complexes that assemble on pre-mRNA. These complexes facilitate the accurate processing of pre-mRNA by guiding the necessary splicing machinery. HnRNP F commonly associates with other proteins such as hnRNP H enhancing its ability to regulate splice site selection and influencing mRNA diversity and function within the cell.

Pathways

HnRNP F is importantly involved in the regulation of gene expression through the mRNA splicing pathway. It particularly affects the spliceosome assembly a multi-protein complex essential for the removal of introns. hnRNP F's involvement in this pathway intersects with proteins such as SF2/ASF and U2AF65 which are key regulators in splicing. Furthermore hnRNP F influences the response to cellular stress pathways by stabilizing specific mRNAs under stress conditions often cooperating with proteins involved in stress granules.

HnRNP F has significant associations with neurodegenerative diseases and cancer. In neurodegenerative diseases aberrant splicing events influenced by hnRNP F are linked to conditions such as Alzheimer's disease where it interacts with splicing factors like SRSF1. In cancer altered expression or malfunction of hnRNP F contributes to splicing variants that promote oncogenesis notably in association with proteins like MYC which drives cancer cell proliferation. These connections make hnRNP F a potential target for therapeutic interventions in disease treatment.
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일반 정보

기능

Component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Plays a role in the regulation of alternative splicing events. Binds G-rich sequences in pre-mRNAs and keeps target RNA in an unfolded state.

Post-translational modifications

Sumoylated.

Subcellular localisation

Nucleus

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제품 프로토콜

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타겟 정보

Component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Plays a role in the regulation of alternative splicing events. Binds G-rich sequences in pre-mRNAs and keeps target RNA in an unfolded state.
See full target information HNRNPF
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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