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AB222951

Recombinant human HPRT protein (Active) (His tag N-Terminus)

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Recombinant human HPRT protein (Active) (His tag N-Terminus) is a Human Full Length protein, in the 1 to 218 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, FuncS, Mass Spec.

대체 명칭 보기

HPRT, HPRT1, Hypoxanthine-guanine phosphoribosyltransferase, HGPRT, HGPRTase

1 이미지
SDS-PAGE - Recombinant human HPRT protein (Active) (His tag N-Terminus) (AB222951)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human HPRT protein (Active) (His tag N-Terminus) (AB222951)

15% SDS-PAGE analysis of 3 μg ab222951.

주요 정보

Purity

>95% SDS-PAGE

ab222951 was purified using conventional chromatography techniques.

발현 시스템

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Specific activity is > 15 units/mg and is defined as the amount of enzyme that catalyze the formation of 1 μmole of guanosine 5'-monophosphate (GMP) per minute from guanine and phosphoribosyl pyrophosphate at pH 7.5 at 37°C.

Accession

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.32% Tris HCl

storage-buffer

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보완 제품

Primary Antibodies

AB10479

Anti-HPRT antibody

KO Validated

Western blot - Anti-HPRT antibody (AB10479)

  • 5
  • 4
Assay Kits

AB112155

Pyrophosphate Assay Kit (Fluorometric)

Functional Studies - Pyrophosphate Assay Kit (Fluorometric) (AB112155)

  • 4
  • 2
Primary Antibodies

AB2900

Anti-EEA1 antibody - Early Endosome Marker

KO Validated|Lab Essentials

Western blot - Anti-EEA1 antibody - Early Endosome Marker (AB2900)

  • 4
  • 39
Primary Antibodies

AB137029

Anti-RAB7 antibody [EPR7589] - Late Endosome Marker

20ul selling size|RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-RAB7 antibody [EPR7589] - Late Endosome Marker (AB137029)

  • 5
  • 25
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"MGSSHHHHHHSSGLVPRGSHMATRSPGVVISDDEPGYDLDLFCIPNHYAEDLERVFIPHGLIMDRTERLARDVMKEMGGHHIVALCVLKGGYKFFADLLDYIKALNRNSDRSIPMTVDFIRLKSYCNDQSTGDIKVIGGDDLSTLTGKNVLIVEDIIDTGKTMQTLLSLVRQYNPKMVKVASLLVKRTPRSVGYKPDFVGFEIPDKFVVGYALDYNEYFRDLNHVCVISETGKAKYKA","proteinLength":"Full Length","predictedMolecularWeight":"26.7 kDa","actualMolecularWeight":null,"aminoAcidEnd":218,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P00492","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
True
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일반 정보

기능

Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway.

서열 유사성

Belongs to the purine/pyrimidine phosphoribosyltransferase family.

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제품 프로토콜

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타겟 정보

Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway.
See full target information HPRT1
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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