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AB314620

Recombinant Human HTR2B Protein (His Tag)

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Recombinant Human HTR2B Protein (His Tag) is a Human Full Length protein, in the 1 to 481 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

대체 명칭 보기

5-hydroxytryptamine receptor 2B, 5-HT-2B, 5-HT2B, Serotonin receptor 2B, HTR2B

1 이미지
SDS-PAGE - Recombinant Human HTR2B Protein (His Tag) (AB314620)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human HTR2B Protein (His Tag) (AB314620)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Observed band size : 70.3 kDa, consistent with the theoretical size.

주요 정보

Purity

>85% SDS-PAGE

발현 시스템

Escherichia coli

Tags

6x His tag N-Terminus SUMO tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Animal free

Yes

Carrier free

No

Species

Human

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%.

보관 버퍼

pH: 7.4 - 8 Constituents: PBS, 6% Trehalose

storage-buffer

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보완 제품

Primary Antibodies

AB194333

Anti-5-HT-2B antibody - Extracellular domain

Western blot - Anti-5-HT-2B antibody - Extracellular domain (AB194333)

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Primary Antibodies

AB134054

Anti-Mu Opioid Receptor antibody [UMB3] - C-terminal

RabMAb|Recombinant|20ul selling size

Western blot - Anti-Mu Opioid Receptor antibody [UMB3] - C-terminal (AB134054)

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Primary Antibodies

AB77517

Anti-GPCR GPR87 antibody

Immunocytochemistry/ Immunofluorescence - Anti-GPCR GPR87 antibody (AB77517)

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Primary Antibodies

AB230778

Anti-5HT1F Receptor antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5HT1F Receptor antibody (AB230778)

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  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"MALSYRVSELQSTIPEHILQSTFVHVISSNWSGLQTESIPEEMKQIVEEQGNKLHWAALLILMVIIPTIGGNTLVILAVSLEKKLQYATNYFLMSLAVADLLVGLFVMPIALLTIMFEAMWPLPLVLCPAWLFLDVLFSTASIMHLCAISVDRYIAIKKPIQANQYNSRATAFIKITVVWLISIGIAIPVPIKGIETDVDNPNNITCVLTKERFGDFMLFGSLAAFFTPLAIMIVTYFLTIHALQKKAYLVKNKPPQRLTWLTVSTVFQRDETPCSSPEKVAMLDGSRKDKALPNSGDETLMRRTSTIGKKSVQTISNEQRASKVLGIVFFLFLLMWCPFFITNITLVLCDSCNQTTLQMLLEIFVWIGYVSSGVNPLVYTLFNKTFRDAFGRYITCNYRATKSVKTLRKRSSKIYFRNPMAENSKFFKKHGIRNGINPAMYQSPMRLRSSTIQSSSIILLDTLLLTENEGDKTEEQVSYV","proteinLength":"Full Length","predictedMolecularWeight":"70.3 kDa","actualMolecularWeight":null,"aminoAcidEnd":481,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P41595","tags":[{"tag":"6x His","terminus":"N-Terminus"},{"tag":"SUMO","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Lyophilized
Purification 테크닉
Ion exchange chromatography
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-80°C
분주 정보
Add glycerol to a final volume of 50% for extra stability and aliquot
보관 정보
The product can be stored for up to 12 months
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

5-HT-2B also known as serotonin receptor 2B is a G-protein-coupled receptor that binds to serotonin. This protein with a molecular mass of approximately 55 kDa is located on the cell surface and exhibits a wide expression in tissues such as the heart lung liver and gastrointestinal tract. It contains several functional domains contributing to its activity mediating cellular responses through signaling cascades when serotonin binds to it.
Biological function summary

5-HT-2B plays a role in modulating the contractility and remodeling of cardiac tissue influencing vascular function and smooth muscle behavior impacting gastrointestinal motility and contributing to liver regeneration. It is not part of a large multiprotein complex but interacts with other cellular components to exert its biological functions. The receptor's activation leads to various intracellular responses influencing different physiological effects depending on the tissue context.

Pathways

Serotonin receptor 2B is involved in the serotonin signaling pathway and the calcium signaling pathway. These pathways impact several physiological processes including mood regulation and cardiovascular function. The receptor interacts with proteins such as phospholipase C which mediates the hydrolysis of phospholipids in the membrane releasing inositol trisphosphate and causing a rise in intracellular calcium levels important for function continuation.

Abnormalities in 5-HT-2B have been linked to cardiac valvulopathy and pulmonary hypertension. These conditions highlight the importance of its role in vascular and cardiac function. The receptor's dysregulation interacts with proteins such as endothelin-1 in vascular abnormalities and contributes to disease progression highlighting its potential as a target for therapeutic interventions in these disorders.
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일반 정보

기능

G-protein coupled receptor for 5-hydroxytryptamine (serotonin) (PubMed : 18703043, PubMed : 23519210, PubMed : 7926008, PubMed : 8078486, PubMed : 8143856, PubMed : 8882600). Also functions as a receptor for various ergot alkaloid derivatives and psychoactive substances (PubMed : 12970106, PubMed : 18703043, PubMed : 23519210, PubMed : 23519215, PubMed : 24357322, PubMed : 28129538, PubMed : 30127358, PubMed : 36087581, PubMed : 7926008, PubMed : 8078486, PubMed : 8143856). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors (PubMed : 23519215, PubMed : 28129538, PubMed : 8078486, PubMed : 8143856, PubMed : 8882600). HTR2B is coupled to G(q)/G(11) G alpha proteins and activates phospholipase C-beta, releasing diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) second messengers that modulate the activity of phosphatidylinositol 3-kinase and promote the release of Ca(2+) ions from intracellular stores, respectively (PubMed : 18703043, PubMed : 23519215, PubMed : 28129538, PubMed : 30127358, PubMed : 36087581, PubMed : 8078486, PubMed : 8143856, PubMed : 8882600). Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways (PubMed : 23519215, PubMed : 28129538, PubMed : 30127358, PubMed : 36087581). Plays a role in the regulation of dopamine and 5-hydroxytryptamine release, 5-hydroxytryptamine uptake and in the regulation of extracellular dopamine and 5-hydroxytryptamine levels, and thereby affects neural activity. May play a role in the perception of pain (By similarity). Plays a role in the regulation of behavior, including impulsive behavior (PubMed : 21179162). Required for normal proliferation of embryonic cardiac myocytes and normal heart development (By similarity). Protects cardiomyocytes against apoptosis (By similarity). Plays a role in the adaptation of pulmonary arteries to chronic hypoxia (By similarity). Plays a role in vasoconstriction (By similarity). Required for normal osteoblast function and proliferation, and for maintaining normal bone density (By similarity). Required for normal proliferation of the interstitial cells of Cajal in the intestine (By similarity).

서열 유사성

Belongs to the G-protein coupled receptor 1 family.

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제품 프로토콜

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타겟 정보

G-protein coupled receptor for 5-hydroxytryptamine (serotonin) (PubMed : 18703043, PubMed : 23519210, PubMed : 7926008, PubMed : 8078486, PubMed : 8143856, PubMed : 8882600). Also functions as a receptor for various ergot alkaloid derivatives and psychoactive substances (PubMed : 12970106, PubMed : 18703043, PubMed : 23519210, PubMed : 23519215, PubMed : 24357322, PubMed : 28129538, PubMed : 30127358, PubMed : 36087581, PubMed : 7926008, PubMed : 8078486, PubMed : 8143856). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors (PubMed : 23519215, PubMed : 28129538, PubMed : 8078486, PubMed : 8143856, PubMed : 8882600). HTR2B is coupled to G(q)/G(11) G alpha proteins and activates phospholipase C-beta, releasing diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) second messengers that modulate the activity of phosphatidylinositol 3-kinase and promote the release of Ca(2+) ions from intracellular stores, respectively (PubMed : 18703043, PubMed : 23519215, PubMed : 28129538, PubMed : 30127358, PubMed : 36087581, PubMed : 8078486, PubMed : 8143856, PubMed : 8882600). Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways (PubMed : 23519215, PubMed : 28129538, PubMed : 30127358, PubMed : 36087581). Plays a role in the regulation of dopamine and 5-hydroxytryptamine release, 5-hydroxytryptamine uptake and in the regulation of extracellular dopamine and 5-hydroxytryptamine levels, and thereby affects neural activity. May play a role in the perception of pain (By similarity). Plays a role in the regulation of behavior, including impulsive behavior (PubMed : 21179162). Required for normal proliferation of embryonic cardiac myocytes and normal heart development (By similarity). Protects cardiomyocytes against apoptosis (By similarity). Plays a role in the adaptation of pulmonary arteries to chronic hypoxia (By similarity). Plays a role in vasoconstriction (By similarity). Required for normal osteoblast function and proliferation, and for maintaining normal bone density (By similarity). Required for normal proliferation of the interstitial cells of Cajal in the intestine (By similarity).
See full target information HTR2B
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Product promise

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