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AB113858

Recombinant human IDH1 protein (His tag N-Terminus)

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(2 제품이 사용된 논문 )

Recombinant human IDH1 protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 414 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec, FuncS.

대체 명칭 보기

PICD, IDH1, Isocitrate dehydrogenase [NADP] cytoplasmic, IDH, Cytosolic NADP-isocitrate dehydrogenase, IDPc, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase

1 이미지
SDS-PAGE - Recombinant human IDH1 protein (His tag N-Terminus) (AB113858)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human IDH1 protein (His tag N-Terminus) (AB113858)

3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

주요 정보

Purity

>95% SDS-PAGE

ab113858 was purified using conventional chromatography.

발현 시스템

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec, FuncS

applications

Biologically active

Yes

Biological activity

Specific activity: > 0.7 units/ml. One unit will convert 1.0 umole of isocitrate to alpha-ketoglutarate per minute at pH7.5 at 25C. Specific activity: > 0.7 units/ml. One unit will convert 1.0 umole of isocitrate to alpha-ketoglutarate per minute at pH7.5 at 25C.

Accession

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF

storage-buffer

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보완 제품

Reagents, Controls & Accessories

AB116027

Prestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa)

SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa) (AB116027)

  • 5
  • 2
Primary Antibodies

AB172964

Anti-IDH1 antibody [EPR12296]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH1 antibody [EPR12296] (AB172964)

  • 5
  • 2
Proteins & Peptides

AB198092

Recombinant human IDH2 protein (DDDDK tag C-Terminus)

Functional Studies - Recombinant human IDH2 protein (DDDDK tag C-Terminus) (AB198092)

  • 0
  • 0
Primary Antibodies

AB181602

Anti-GAPDH antibody [EPR16891] - Loading Control

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [EPR16891] - Loading Control (AB181602)

  • 5
  • 23
Assay Kits

AB102528

Isocitrate Dehydrogenase Assay Kit (Colorimetric)

Functional Studies - Isocitrate Dehydrogenase Assay Kit (Colorimetric) (AB102528)

  • 4
  • 1
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

This product was previously labelled as Isocitrate dehydrogenase
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서열 정보

[{"linker":null,"sequence":"MGSSHHHHHHSSGLVPRGSHMSKKISGGSVVEMQGDEMTRIIWELIKEKLIFPYVELDLHSYDLGIENRDATNDQVTKDAAEAIKKHNVGVKCATITPDEKRVEEFKLKQMWKSPNGTIRNILGGTVFREAIICKNIPRLVSGWVKPIIIGRHAYGDQYRATDFVVPGPGKVEITYTPSDGTQKVTYLVHNFEEGGGVAMGMYNQDKSIEDFAHSSFQMALSKGWPLYLSTKNTILKKYDGRFKDIFQEIYDKQYKSQFEAQKIWYEHRLIDDMVAQAMKSEGGFIWACKNYDGDVQSDSVAQGYGSLGMMTSVLVCPDGKTVEAEAAHGTVTRHYRMYQKGQETSTNPIASIFAWTRGLAHRAKLDNNKELAFFANALEEVSIETIEAGFMTKDLAACIKGLPNVQRSDYLNTFEFMDKLGENLKIKLAQAKL","proteinLength":"Full Length","predictedMolecularWeight":"48.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":414,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O75874","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
True
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

IDH1 also known as isocitrate dehydrogenase 1 is an enzyme with a molecular weight of approximately 45 kDa. It participates in the citric acid cycle aiding the conversion of isocitrate to alpha-ketoglutarate while producing NADPH. IDH1 resides primarily in the cytoplasm and peroxisomes of cells. It finds expression in various tissues most notably the liver and brain where metabolic processes are highly active.
Biological function summary

Isocitrate dehydrogenase 1 plays an important role in cellular metabolism by helping to maintain the NADP+/NADPH balance important for redox reactions. This enzyme operates mainly as a homodimer meaning two identical subunits form a complex for functional activity. It contributes significantly to lipid and glucose metabolism due to its production of NADPH which the cells use for biosynthetic pathways and detoxification.

Pathways

The involvement of IDH1 in the citric acid cycle highlights its importance in energy production and metabolic regulation. This enzyme closely associates with other metabolic enzymes such as IDH2 another member of the isocitrate dehydrogenase family found in mitochondria suggesting a coordinated function between cytoplasmic and mitochondrial metabolism. Beyond its role in the citric acid cycle IDH1 also relates to the pentose phosphate pathway an alternative glucose metabolism route providing reducing equivalents and ribose 5-phosphate for nucleotide synthesis.

IDH1 mutations particularly the IDH1 R132H variant frequently appear in certain cancers like gliomas and acute myeloid leukemia (AML). These mutations lead to a neomorphic enzyme function producing 2-hydroxyglutarate instead of alpha-ketoglutarate disrupting normal cellular metabolism and aiding tumorigenesis. The mutated IDH1 also affects the TET2 protein which involves in DNA demethylation potentially altering gene expression and contributing to oncogenic pathways.
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일반 정보

기능

Catalyzes the NADP(+)-dependent oxidative decarboxylation of isocitrate (D-threo-isocitrate) to 2-ketoglutarate (2-oxoglutarate), which is required by other enzymes such as the phytanoyl-CoA dioxygenase (PubMed : 10521434, PubMed : 19935646). Plays a critical role in the generation of NADPH, an important cofactor in many biosynthesis pathways (PubMed : 10521434). May act as a corneal epithelial crystallin and may be involved in maintaining corneal epithelial transparency (By similarity).

서열 유사성

Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Post-translational modifications

Acetylation at Lys-374 dramatically reduces catalytic activity.

Subcellular localisation

Peroxisome

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제품 프로토콜

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타겟 정보

Catalyzes the NADP(+)-dependent oxidative decarboxylation of isocitrate (D-threo-isocitrate) to 2-ketoglutarate (2-oxoglutarate), which is required by other enzymes such as the phytanoyl-CoA dioxygenase (PubMed : 10521434, PubMed : 19935646). Plays a critical role in the generation of NADPH, an important cofactor in many biosynthesis pathways (PubMed : 10521434). May act as a corneal epithelial crystallin and may be involved in maintaining corneal epithelial transparency (By similarity).
See full target information IDH1
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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Molecules (Basel, Switzerland) 27: PubMed35744895

2022

Novel Radioiodinated and Radiofluorinated Analogues of FT-2102 for SPECT or PET Imaging of mIDH1 Mutant Tumours.

Applications

Unspecified application

Species

Unspecified reactive species

Valérie Weber,Lucie Arnaud,Sladjana Dukic-Stefanovic,Barbara Wenzel,Valérie Roux,Jean-Michel Chezal,Thu-Hang Lai,Rodrigo Teodoro,Klaus Kopka,Elisabeth Miot-Noirault,Winnie Deuther-Conrad,Aurélie Maisonial-Besset

Nature chemical biology 13:494-500 PubMed28263965

2017

L-2-Hydroxyglutarate production arises from noncanonical enzyme function at acidic pH.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew M Intlekofer,Bo Wang,Hui Liu,Hardik Shah,Carlos Carmona-Fontaine,Ariën S Rustenburg,Salah Salah,M R Gunner,John D Chodera,Justin R Cross,Craig B Thompson
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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