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AB323023

Recombinant Human Iduronate 2 sulfatase/SIDS Protein Standard

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Recombinant Human Iduronate 2 sulfatase/SIDS Protein Standard is a Human Full Length protein, expressed in HEK 293 cells, with >80%, suitable for sELISA, SDS-PAGE.
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Sandwich ELISA - Recombinant Human Iduronate 2 sulfatase/SIDS Protein Standard (AB323023)
  • sELISA

Supplier Data

Sandwich ELISA - Recombinant Human Iduronate 2 sulfatase/SIDS Protein Standard (AB323023)

Sandwich ELISA with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

SDS-PAGE - Recombinant Human Iduronate 2 sulfatase/SIDS Protein Standard (AB323023)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human Iduronate 2 sulfatase/SIDS Protein Standard (AB323023)

SDS-PAGE analysis of ab323023 under reducing conditions for 2ug protein.

주요 정보

Purity

>80% SDS-PAGE

발현 시스템

HEK 293 cells

Tags

Tag free

Applications

sELISA, SDS-PAGE

applications

Biologically active

No

Accession

P22304-1

Animal free

Yes

Carrier free

No

Species

Human

보관 버퍼

pH: 7.3 - 7.5 Constituents: 2.922% Sodium chloride, 0.64107% disodium;hydrogen phosphate;dodecahydrate, 0.02858% Potassium phosphate monobasic

storage-buffer

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보완 제품

Reagents, Controls & Accessories

AB234617

Prestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa)

SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa) (AB234617)

  • 0
  • 0
Cell Lines & Lysates

AB266378

Human ALDH18A1 (P5CS) knockout HEK-293T cell line

Sanger Sequencing - Human ALDH18A1 (P5CS) knockout HEK-293T cell line (AB266378)

  • 0
  • 0
Primary Antibodies

AB316988

Anti-Iduronate 2 sulfatase/SIDS antibody [EPR25954-155] - BSA and Azide free (Detector)

Recombinant|RabMAb

Sandwich ELISA - Anti-Iduronate 2 sulfatase/SIDS antibody [EPR25954-155] - BSA and Azide free (Detector) (AB316988)

  • 0
  • 0
Assay Kits

AB282882

Mouse ALT ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Mouse ALT ELISA Kit (AB282882)

  • 5
  • 1
Assay Kits

AB179887

Human Albumin ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Albumin ELISA Kit (AB179887)

  • 3
  • 1
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Support team to know which antibody pair is suitable for this protein.
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서열 정보

[{"linker":null,"sequence":null,"proteinLength":"Full Length","predictedMolecularWeight":"59.7 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P22304","tags":[]}]
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification His Tag
배송 시 보관 조건
Dry Ice
적절한 단기 보관 조건
-80°C
적절한 장기 보관 조건
-80°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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일반 정보

기능

Lysosomal enzyme involved in the degradation pathway of dermatan sulfate and heparan sulfate.

서열 유사성

Belongs to the sulfatase family.

Post-translational modifications

Synthesized as a 75-kDa precursor form in the endoplasmic reticulum (ER), and then processed by proteolytic cleavage through various intermediates to yield a 55-kDa mature form, with the release of an 18 kDa polypeptide.. The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity.

Subcellular localisation

Lysosome

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제품 프로토콜

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타겟 정보

Lysosomal enzyme involved in the degradation pathway of dermatan sulfate and heparan sulfate.
See full target information IDS

대체 명칭 보기

SIDS, IDS, Iduronate 2-sulfatase, Alpha-L-iduronate sulfate sulfatase, Idursulfase
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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