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AB259403

Recombinant human IL-15 protein (Active)

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Recombinant human IL-15 protein (Active) is a Human Full Length protein, in the 49 to 162 aa range, expressed in HEK 293 cells, with >95%, < 0.005 EU/µg endotoxin level, suitable for sELISA, SDS-PAGE, FuncS, Mass Spec, HPLC, Cell Culture.
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Functional Studies - Recombinant human IL-15 protein (Active) (AB259403)
  • FuncS

Supplier Data

Functional Studies - Recombinant human IL-15 protein (Active) (AB259403)

Functional analysis of ab259403.

Fully biologically active determined by the dose dependent proliferation of KHYG-1 cells.

ED50 is ≤1.28 ng/mL, corresponding to a specific activity of 7.8 x 105 units/mg.

Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.

Lot : GR3379485-1

Mass Spectrometry - Recombinant human IL-15 protein (Active) (AB259403)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant human IL-15 protein (Active) (AB259403)

Mass determination by ESI-TOF.

Predicted MW is 12830.92 Da (+/- 10 Da by ESI-TOF). Observed MW is 12832.38 Da.

Sandwich ELISA - Recombinant human IL-15 protein (Active) (AB259403)
  • sELISA

Lab

Sandwich ELISA - Recombinant human IL-15 protein (Active) (AB259403)

Background subtracted standard curve using Human IL-15 Antibody Pair - BSA and Azide free (ab241883) and Recombinant human IL-15 protein (Active) (ab259403) in sandwich ELISA.
The ELISA was performed using the components of the corresponding SimpleStep® kit, which uses the same antibody pair with a different formulation and format.

HPLC - Recombinant human IL-15 protein (Active) (AB259403)
  • HPLC

Supplier Data

HPLC - Recombinant human IL-15 protein (Active) (AB259403)

HPLC analysis of ab259403.

SDS-PAGE - Recombinant human IL-15 protein (Active) (AB259403)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human IL-15 protein (Active) (AB259403)

SDS-PAGE analysis of ab259403.

주요 정보

Purity

>95% SDS-PAGE

>= 95 % HPLC.

Endotoxin 레벨

< 0.005 EU/µg

발현 시스템

HEK 293 cells

Tags

Tag free

Applications

FuncS, sELISA, Cell Culture, Mass Spec, SDS-PAGE, HPLC

applications

Biologically active

Yes

Biological activity

Fully biologically active determined by the dose dependent proliferation of KHYG-1 cells. <p>ED<sub>50</sub> is &le;1.28 ng/mL, corresponding to a specific activity of 7.8 x 10<sup>5</sup> units/mg.</p>

Accession

P40933-1

Animal free

Yes

Carrier free

No

Species

Human

Reconstitution

Reconstitute in PBS

보관 버퍼

pH: 6 - 8 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Cell Culture": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

제품 세부 정보

This protein is filter sterilised prior to aliquoting and lyophilisation. All aliquoting and lyophilisation steps are performed in a sterile environment

서열 정보

[{"linker":null,"sequence":"NWVNVISDLKKIEDLIQSMHIDATLYTESDVHPSCKVTAMKCFLLELQVISLESGDASIHDTVENLIILANNSLSSNGNVTESGCKECEELEEKNIKEFLQSFVHIVQMFINTS","proteinLength":"Full Length","predictedMolecularWeight":"12.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":162,"aminoAcidStart":49,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P40933","tags":[]}]

특성 및 보관 정보

제형
Lyophilized
배송 시 보관 조건
Ambient - Can Ship with Ice
적절한 단기 보관 조건
Ambient
적절한 장기 보관 조건
Ambient
True

제품 프로토콜

타겟 정보

Cytokine that plays a major role in the development of inflammatory and protective immune responses to microbial invaders and parasites by modulating immune cells of both the innate and adaptive immune systems (PubMed : 15123770). Stimulates the proliferation of natural killer cells, T-cells and B-cells and promotes the secretion of several cytokines (PubMed : 8178155, PubMed : 9326248). In monocytes, induces the production of IL8 and monocyte chemotactic protein 1/CCL2, two chemokines that attract neutrophils and monocytes respectively to sites of infection (PubMed : 9326248). Unlike most cytokines, which are secreted in soluble form, IL15 is expressed in association with its high affinity IL15RA on the surface of IL15-producing cells and delivers signals to target cells that express IL2RB and IL2RG receptor subunits (PubMed : 10233906, PubMed : 23104097, PubMed : 8026467). Binding to its receptor triggers the phosphorylation of JAK1 and JAK3 and the recruitment and subsequent phosphorylation of signal transducer and activator of transcription-3/STAT3 and STAT5 (PubMed : 7568001). In mast cells, induces the rapid tyrosine phosphorylation of STAT6 and thereby controls mast cell survival and release of cytokines such as IL4 (By similarity).
See full target information IL15

대체 명칭 보기

Interleukin-15, IL-15, IL15

제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록and refine your search

eLife 10: PubMed33522483

2021

Evolutionary transcriptomics implicates in the origins of implantation and regulation of gestation length.

Applications

Unspecified application

Species

Unspecified reactive species

Mirna Marinić,Katelyn Mika,Sravanthi Chigurupati,Vincent J Lynch
제품이 사용된 논문 모두 보기

Product promise

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