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AB310799

Recombinant Human IL-18 Protein (His tag) (Active)

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Recombinant Human IL-18 Protein (His tag) (Active) is a Human Full Length protein, in the 37 to 193 aa range, expressed in Escherichia coli, with >95%, <0.1 EU/mg endotoxin level, suitable for Mass Spec, SDS-PAGE, HPLC, Cell Culture, FuncS.
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Functional Studies - Recombinant Human IL-18 Protein (His tag) (Active) (AB310799)
  • FuncS

Supplier Data

Functional Studies - Recombinant Human IL-18 Protein (His tag) (Active) (AB310799)

Fully biologically active determined by the dose dependent NF-κB and AP-1 pathway activation of human embryonic kidney (HEK)- 293 cells overexpressing IL-18R and IL-18RAP inducible SEAP reporter genes. Results obtained with Invivogen HEK BlueTM IL-18 cells (Cat code., hkb-hmil18). ED50 ≤ 0.03 ng/ml, corresponding to a specific activity of 3.3 x 10^7 units/mg.

Mass Spectrometry - Recombinant Human IL-18 Protein (His tag) (Active) (AB310799)
  • Mass Spec

Lab

Mass Spectrometry - Recombinant Human IL-18 Protein (His tag) (Active) (AB310799)

Mass determination by ESI-TOF. Predicted MW is 19412.29118 (+/- 10Da by ESI-TOF). Observed MW is 19412.26.

SDS-PAGE - Recombinant Human IL-18 Protein (His tag) (Active) (AB310799)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human IL-18 Protein (His tag) (Active) (AB310799)

SDS-PAGE analysis of ab310799

SDS-PAGE - Recombinant Human IL-18 Protein (His tag) (Active) (AB310799)
  • SDS-PAGE

Lab

SDS-PAGE - Recombinant Human IL-18 Protein (His tag) (Active) (AB310799)

SDS-PAGE analysis of ab310799

주요 정보

Purity

>95% HPLC

SDS-PAGE >= 95%

Endotoxin 레벨

<0.1 EU/mg

발현 시스템

Escherichia coli

Tags

His tag C-Terminus

Applications

Cell Culture, SDS-PAGE, Mass Spec, HPLC, FuncS

applications

Biologically active

Yes

Biological activity

Fully biologically active determined by the dose dependent NF-κB and AP-1 pathway activation of human embryonic kidney (HEK)- 293 cells overexpressing IL-18R and IL-18RAP inducible SEAP reporter genes. Results obtained with Invivogen HEK BlueTM IL-18 cells (Cat code., hkb-hmil18). ED50 ≤ 0.03 ng/ml, corresponding to a specific activity of 3.3 x 10<sup>7</sup> units/mg.

Accession

Q14116-1

Animal free

Yes

Carrier free

No

Species

Human

Reconstitution

Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product. Store lyophilized form at room temperature. Reconstitute in phosphate buffered saline, aliquot and store at -80°C for 12 months or +4°C for 1 week. Avoid repeated freeze-thaw.

보관 버퍼

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

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보완 제품

Reagents, Controls & Accessories

AB313995

Prestained Protein Ladder - Mid-range molecular weight (15 - 170 kDa)

SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (15 - 170 kDa) (AB313995)

  • 0
  • 0
Assay Kits

AB215539

Human IL-18 ELISA Kit

Recombinant|SimpleStep

ELISA - Human IL-18 ELISA Kit (AB215539)

  • 5
  • 1
Proteins & Peptides

AB259387

Recombinant human IL-1 beta protein (Active)

Premium Bioactive

Sandwich ELISA - Recombinant human IL-1 beta protein (Active) (AB259387)

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Proteins & Peptides

AB282392

Recombinant human IL-17A protein (Active)

Premium Bioactive

Functional Studies - Recombinant human IL-17A protein (Active) (AB282392)

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Assay Kits

AB218808

Mouse IL-18 Matched Antibody Pair Kit

Recombinant

ELISA - Mouse IL-18 Matched Antibody Pair Kit (AB218808)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Cell Culture": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"MYFGKLESKLSVIRNLNDQVLFIDQGNRPLFEDMTDSDCRDNAPRTIFIISMYKDSQPRGMAVTISVKCEKISTLSCENKIISFKEMNPPDNIKDTKSDIIFFQRSVPGHDNKMQFESSSYEGYFLACEKERDLFKLILKKEDELGDRSIMFTVQNED","proteinLength":"Full Length","predictedMolecularWeight":"19.4 kDa","actualMolecularWeight":"19.4 kDa","aminoAcidEnd":193,"aminoAcidStart":37,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q14116","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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특성 및 보관 정보

제형
Lyophilized
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
Ambient
적절한 장기 보관 조건
Ambient
True
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제품 프로토콜

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타겟 정보

Pro-inflammatory cytokine primarily involved in epithelial barrier repair, polarized T-helper 1 (Th1) cell and natural killer (NK) cell immune responses (PubMed : 10653850). Upon binding to IL18R1 and IL18RAP, forms a signaling ternary complex which activates NF-kappa-B, triggering synthesis of inflammatory mediators (PubMed : 14528293, PubMed : 25500532, PubMed : 37993714). Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells and natural killer (NK) cells (PubMed : 10653850). Involved in transduction of inflammation downstream of pyroptosis : its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore (PubMed : 33883744).
See full target information IL18

대체 명칭 보기

IGIF, IL1F4, IL18, Interleukin-18, IL-18, Iboctadekin, Interferon gamma-inducing factor, Interleukin-1 gamma, IFN-gamma-inducing factor, IL-1 gamma
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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