Recombinant Human IL-33 protein (Active)
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Recombinant Human IL-33 protein (Active) is a Human Full Length protein, in the 95 to 270 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for SDS-PAGE, Mass Spec, HPLC, FuncS, Cell Culture.
대체 명칭 보기
C9orf26, IL1F11, NFHEV, IL33, Interleukin-33, IL-33, Interleukin-1 family member 11, Nuclear factor from high endothelial venules, IL-1F11, NF-HEV
- FuncS
Supplier Data
Functional Studies - Recombinant Human IL-33 protein (Active) (AB281811)
Recombinant Human IL-33 protein was determined to be fully biologically active by the dose dependent proliferation of D10S cells. ED50 is ≤ 1.70 ng/ml, corresponding to a specific activity of 5.9 x 10⁵ units/mg.
Cell based assay testing is performed on the first lot of the protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.
Lot GR3425563-1.
- Mass Spec
Supplier Data
Mass Spectrometry - Recombinant Human IL-33 protein (Active) (AB281811)
ESI-TOF analysis of ab281811.
Predicted MW is 19883.29 Da (+/- 10 Da by ESI-TOF). Observed MW is 19884.54 Da.
- Biochemical assay
Supplier Data
Biochemical assay - Recombinant Human IL-33 protein (Active) (AB281811)
Fully biologically active determined by the dose dependent proliferation of D10S cells. ED50 <= 1.70 ng/ml, corresponding to a specific activity of 5.9x105 units/mg.
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant Human IL-33 protein (Active) (AB281811)
SDS-PAGE analysis of ab281811.
- HPLC
Supplier Data
HPLC - Recombinant Human IL-33 protein (Active) (AB281811)
HPLC analysis of ab281811.
보완 제품
Reagents, Controls & Accessories
AB325997
Prestained Protein Ladder - Extra broad molecular weight (3-250 kDa, 5-color)
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Reactivity 정보
서열 정보
특성 및 보관 정보
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일반 정보
기능
Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells (PubMed : 16286016, PubMed : 19841166). Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines (PubMed : 17853410, PubMed : 18836528). Also involved in activation of mast cells, basophils, eosinophils and natural killer cells (PubMed : 17853410, PubMed : 18836528). Acts as an enhancer of polarization of alternatively activated macrophages (PubMed : 19841166). Acts as a chemoattractant for Th2 cells, and may function as an 'alarmin', that amplifies immune responses during tissue injury (PubMed : 17853410, PubMed : 18836528). Induces rapid UCP2-dependent mitochondrial rewiring that attenuates the generation of reactive oxygen species and preserves the integrity of Krebs cycle required for persistent production of itaconate and subsequent GATA3-dependent differentiation of inflammation-resolving alternatively activated macrophages (By similarity).. In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets (PubMed : 21734074). This form is rapidely lost upon angiogenic or pro-inflammatory activation (PubMed : 18787100).
서열 유사성
Belongs to the IL-1 family. Highly divergent.
Post-translational modifications
The full-length protein can be released from cells and is able to signal via the IL1RL1/ST2 receptor. However, proteolytic processing by CELA1, CSTG/cathepsin G and ELANE/neutrophil elastase produces C-terminal peptides that are more active than the unprocessed full length protein (PubMed:22307629, PubMed:35794369). May also be proteolytically processed by calpains (PubMed:19596270, PubMed:22307629). Proteolytic cleavage mediated by apoptotic caspases including CASP3 and CASP7 results in IL33 inactivation (PubMed:19559631). In vitro proteolytic cleavage by CASP1 was reported (PubMed:16286016, PubMed:19439663) but could not be confirmed in vivo (PubMed:19465481) suggesting that IL33 is probably not a direct substrate for that caspase (PubMed:19439663, PubMed:19465481).
Subcellular localisation
Nucleus
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