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AB42619

Recombinant human JAK2 protein (His tag N-Terminus)

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(5 제품이 사용된 논문 )

Recombinant human JAK2 protein (His tag N-Terminus) is a Human Fragment protein, in the 808 to 1132 aa range, expressed in Baculovirus infected Sf9 cells, with >70%, suitable for SDS-PAGE, FuncS, FuncS (Neut/Block).
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Functional Studies - Recombinant human JAK2 protein (His tag N-Terminus) (AB42619)
  • FuncS

Supplier Data

Functional Studies - Recombinant human JAK2 protein (His tag N-Terminus) (AB42619)

Assay was done in Kinase buffer containing 1 mM DTT using Poly-(Glu4 : Tyr) substrate (0.2 mg/ml) and 20 μM ATP. Reaction was done at 30oC for 45 min. Amount of ATP transferred was calculated using Kinase-Glo reagent.

SDS-PAGE - Recombinant human JAK2 protein (His tag N-Terminus) (AB42619)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human JAK2 protein (His tag N-Terminus) (AB42619)

Lane 1 : 2μg ab42619

Lane 2 : Protein marker

Observed band : 43 kDa

주요 정보

Purity

>70% SDS-PAGE

Affinity purified.

발현 시스템

Baculovirus infected Sf9 cells

Tags

His tag N-Terminus

Applications

FuncS, FuncS (Neut/Block), SDS-PAGE

applications

Biologically active

Yes

Biological activity

Activity: &gt; 124 pmol/min/ug and is lot specific. If you would like more information about the activity of a specific lot, please contact our scientific support team who will be happy to help.<br>One unit defined as the amount of enzyme that will transfer 1nmol phosphate to Tyr substrate per minute at pH 7.4 and 30°C.

Accession

O60674-1

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 8 Constituents: 50% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.395% Tris HCl, 0.05% Sorbitan monolaurate, ethoxylated, 0.0462% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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보완 제품

Reagents, Controls & Accessories

AB133411

Low Fluorescence Western Membrane (PVDF)

  • 5
  • 1
Proteins & Peptides

AB89982

Recombinant human JAK3 protein (Active) (His tag N-Terminus)

Functional Studies - Recombinant human JAK3 protein (Active) (His tag N-Terminus) (AB89982)

  • 0
  • 0
Proteins & Peptides

AB271754

Recombinant human Tet2 protein (Active) (6x His tag C-Terminus + DDDDK N-Terminus)

Functional Studies - Recombinant human Tet2 protein (Active) (6x His tag C-Terminus + DDDDK N-Terminus) (AB271754)

  • 0
  • 0
Proteins & Peptides

AB43618

Recombinant Human STAT3 protein (GST tag N-Terminus)

Western blot - Recombinant Human STAT3 protein (GST tag N-Terminus) (AB43618)

  • 0
  • 0
Primary Antibodies

AB108596

Anti-JAK2 antibody [EPR108(2)]

20ul selling size|RabMAb|Recombinant|KO Validated|Trial Size

Immunocytochemistry/ Immunofluorescence - Anti-JAK2 antibody [EPR108(2)] (AB108596)

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  • 3
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS (Neut/Block)": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Source : Baculovirus infected Sf9 cells
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서열 정보

[{"linker":null,"sequence":"MSPIDPMGHHHHHHGRRRASVAAGILVPRGSPGLDGICSIEEFLFTPDYELLTENDMLPNMRIGALGFSGAFEDRDPTQFEERHLKFLQQLGKGNFGSVEMCRYDPLQDNTGEVVAVKKLQHSTEEHLRDFEREIEILKSLQHDNIVKYKGVCYSAGRRNLKLIMEYLPYGSLRDYLQKHKERIDHIKLLQYTSQICKGMEYLGTKRYIHRDLATRNILVENENRVKIGDFGLTKVLPQDKEYYKVKEPGESPIFWYAPESLTESKFSVASDVWSFGVVLYELFTYIEKSKSPPAEFMRMIGNDKQGQMIVFHLIELLKNNGRLPRPDGCPDEIYMIMTECWNNNVNQRPSFRDLALRVDQIRDNMAG","proteinLength":"Fragment","predictedMolecularWeight":"43 kDa","actualMolecularWeight":null,"aminoAcidEnd":1132,"aminoAcidStart":808,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"O60674","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification
배송 시 보관 조건
Dry Ice
적절한 단기 보관 조건
-80°C
적절한 장기 보관 조건
-80°C
보관 정보
Avoid freeze / thaw cycle
True
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

JAK2 also known as Janus kinase 2 is a protein tyrosine kinase with a molecular weight of 125 kDa. It plays an essential mechanical role in the signaling pathways by acting as an intermediary between cell surface receptors and intracellular signaling molecules. The JAK2 protein binds to certain cytokine receptors facilitating signal transduction necessary for various cellular responses. JAK2 is expressed in many tissues including hematopoietic cells which are associated with the blood and immune systems.
Biological function summary

JAK2 is important for transmitting signals that dictate cell growth survival and differentiation within the hematopoietic system. It operates bodily functions by forming complexes with specific phosphorylation sites on its associated receptors. Through this formation JAK2 influences the signaling cascade particularly by interacting with other signal transducers and activators where it phosphorylates and becomes activated. This action affects gene transcription directly correlated with cellular proliferation and differentiation processes.

Pathways

The function of JAK2 integrates into the JAK-STAT signaling pathway which is an important pathway in the regulation of immune function growth and development. It works in conjunction with proteins such as STAT3 and STAT5 to transmit signals from cytokine receptors to the nucleus. This pathway critically impacts responses like inflammation and hematopoiesis aligning with its role in precursor proliferation within the bone marrow and various immune cells’ function.

JAK2 has significant implications in conditions like myeloproliferative neoplasms and polycythemia vera. Mutations in the JAK2 gene notably the JAK2 V617F mutation lead to uncontrolled cell division as they disrupt normal signaling mechanisms often resulting in blood cell disorders. In these contexts JAK2 interacts with proteins such as EpoR and MPL which play roles within these disease pathways. Understanding how JAK2 mutations contribute to disease progression offers pathways for targeted therapies.
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제품 프로토콜

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타겟 정보

Non-receptor tyrosine kinase involved in various processes such as cell growth, development, differentiation or histone modifications. Mediates essential signaling events in both innate and adaptive immunity. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors such as growth hormone (GHR), prolactin (PRLR), leptin (LEPR), erythropoietin (EPOR), thrombopoietin receptor (MPL/TPOR); or type II receptors including IFN-alpha, IFN-beta, IFN-gamma and multiple interleukins (PubMed : 15690087, PubMed : 7615558, PubMed : 9657743, PubMed : 15899890). Following ligand-binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins (PubMed : 15690087, PubMed : 9618263). Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, cell stimulation with erythropoietin (EPO) during erythropoiesis leads to JAK2 autophosphorylation, activation, and its association with erythropoietin receptor (EPOR) that becomes phosphorylated in its cytoplasmic domain (PubMed : 9657743). Then, STAT5 (STAT5A or STAT5B) is recruited, phosphorylated and activated by JAK2. Once activated, dimerized STAT5 translocates into the nucleus and promotes the transcription of several essential genes involved in the modulation of erythropoiesis. Part of a signaling cascade that is activated by increased cellular retinol and that leads to the activation of STAT5 (STAT5A or STAT5B) (PubMed : 21368206). In addition, JAK2 mediates angiotensin-2-induced ARHGEF1 phosphorylation (PubMed : 20098430). Plays a role in cell cycle by phosphorylating CDKN1B (PubMed : 21423214). Cooperates with TEC through reciprocal phosphorylation to mediate cytokine-driven activation of FOS transcription. In the nucleus, plays a key role in chromatin by specifically mediating phosphorylation of 'Tyr-41' of histone H3 (H3Y41ph), a specific tag that promotes exclusion of CBX5 (HP1 alpha) from chromatin (PubMed : 19783980). Up-regulates the potassium voltage-gated channel activity of KCNA3 (PubMed : 25644777).
See full target information JAK2

대체 명칭 보기

Tyrosine-protein kinase JAK2, Janus kinase 2, JAK-2, JAK2
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제품이 사용된 논문 (5)

Recent publications for all applications. Explore the 전체 목록and refine your search

Nature communications 15:3415 PubMed38649367

2024

Tyrosine phosphorylation of CARM1 promotes its enzymatic activity and alters its target specificity.

Applications

Unspecified application

Species

Unspecified reactive species

Hidehiro Itonaga,Adnan K Mookhtiar,Sarah M Greenblatt,Fan Liu,Concepcion Martinez,Daniel Bilbao,Masai Rains,Pierre-Jacques Hamard,Jun Sun,Afoma C Umeano,Stephanie Duffort,Chuan Chen,Na Man,Gloria Mas,Luca Tottone,Tulasigeri Totiger,Terrence Bradley,Justin Taylor,Stephan Schürer,Stephen D Nimer

Oncogenesis 12:24 PubMed37147297

2023

Vortioxetine hydrobromide inhibits the growth of gastric cancer cells in vivo and in vitro by targeting JAK2 and SRC.

Applications

Unspecified application

Species

Unspecified reactive species

Mingzhu Li,Lina Duan,Wenjie Wu,Wenjing Li,Lili Zhao,Ang Li,Xuebo Lu,Xinyu He,Zigang Dong,Kangdong Liu,Yanan Jiang

The Journal of pharmacology and experimental therapeutics 379:147-155 PubMed34400527

2021

Puerarin Ameliorates 5-Fluorouracil-Induced Intestinal Mucositis in Mice by Inhibiting JAKs.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Wang,Baohui Song,Yan Hu,Jun Chen,Shuaishuai Zhang,Dapeng Chen,Jingyu Wang

Theranostics 8:676-692 PubMed29344298

2018

AKR1C1 Activates STAT3 to Promote the Metastasis of Non-Small Cell Lung Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Zhu,Lin-Lin Chang,Fang-Jie Yan,Yan Hu,Chen-Ming Zeng,Tian-Yi Zhou,Tao Yuan,Mei-Dan Ying,Ji Cao,Qiao-Jun He,Bo Yang

Molecular and cellular biology 35:111-31 PubMed25332239

2014

JAK2 tyrosine kinase phosphorylates and is negatively regulated by centrosomal protein Ninein.

Applications

Unspecified application

Species

Unspecified reactive species

Jennifer Jay,Alan Hammer,Andrea Nestor-Kalinoski,Maria Diakonova
제품이 사용된 논문 모두 보기
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Product promise

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