AB93699

Recombinant human Lactate Dehydrogenase protein (Active) (His tag N-Terminus)

Be the first to review this product! 리뷰 제출

(5 제품이 사용된 논문 )

Recombinant human Lactate Dehydrogenase protein (Active) (His tag N-Terminus) is a Human Full Length protein, in the 1 to 332 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, WB, FuncS.

2 이미지

Unknown

Western blot - Recombinant human Lactate Dehydrogenase protein (Active) (His tag N-Terminus) (AB93699)

All lanes:

Western blot - Anti-Lactate Dehydrogenase antibody (<a href='/ko/products/primary-antibodies/lactate-dehydrogenase-antibody-ab47010'>ab47010</a>) at 1 µg/mL

All lanes:

Western blot - Recombinant human Lactate Dehydrogenase protein (Active) (His tag N-Terminus) (ab93699) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 37 kDa

true

Exposure time: 10s

SDS-PAGE - Recombinant human Lactate Dehydrogenase protein (Active) (His tag N-Terminus) (AB93699)

SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human Lactate Dehydrogenase protein (Active) (His tag N-Terminus) (AB93699)

SDS-PAGE analysis of Recombinant Human Lactate Dehydrogenase protein (ab93699), under reducing conditions. Proteins visualized by coomassie blue stain.

Lane 1 : Molecular Weight Standards

Lane 2 : 3 μg Recombinant Human Lactate Dehydrogenase protein

주요 정보

Purity

>95% SDS-PAGE

ab93699 is purified by using conventional chromatography techniques.

발현 시스템

Escherichia coli

Applications

SDS-PAGE, WB, FuncS

applications

Biologically active

Yes

Biological activity

Specific activity is > 300 units/mg. in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37°C. Activity Assay <ol> <li>Prepare a 1.5 ml reaction mix into a suitable container, adjust to pH 7.5 at 37°C and pre-chill on ice before use: The final concentrations are 100mM Sodium phosphate, 0.12 mM beta-nicotinamide adenine dinucleotide, reduced form, 2.3mM Pyruvate, 0.033% (w/v) bovine serum albumin.</li> <li>Equilibrate to 37°C and monitor at A340nm until the value is constant using a spectrophotometer.</li> <li>Add 50ul of recombinant LDHA protein in various concentrations (0.1ug, 0.5ug) in assay buffer.</li> <li>Mix by inversion and record the increase at A340nm for 5 minutes.</li> </ol>

Accession

P00338-1

Animal free

Carrier free

Species

Human

보관 버퍼

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl

storage-buffer

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"ab93699 can be used as a WB positive control in conjunction with <a href='/ko/products/primary-antibodies/lactate-dehydrogenase-antibody-ab47010'>ab47010</a>." }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" } } }

style

left-column

제품 세부 정보

Concentration determined by Bradford assay.

style

left-column

서열 정보

[{"linker":null,"sequence":"MGSSHHHHHHSSGLVPRGSHMATLKDQLIYNLLKEEQTPQNKITVVGVGAVGMACAISILMKDLADELALVDVIEDKLKGEMMDLQHGSLFLRTPKIVSGKDYNVTANSKLVIITAGARQQEGESRLNLVQRNVNIFKFIIPNVVKYSPNCKLLIVSNPVDILTYVAWKISGFPKNRVIGSGCNLDSARFRYLMGERLGVHPLSCHGWVLGEHGDSSVPVWSGMNVAGVSLKTLHPDLGTDKDKEQWKEVHKQVVESAYEVIKLKGYTSWAIGLSVADLAESIMKNLRRVHPVSTMIKGLYGIKDDVFLSVPCILGQNGISDLVKVTLTSEEEARLKKSADTLWGIQKELQF","proteinLength":"Full Length","predictedMolecularWeight":"38.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":332,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P00338","tags":[{"tag":"His","terminus":"N-Terminus"}]}]

style

left-column

특성 및 보관 정보

제형

Liquid

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

True

style

left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Lactate dehydrogenase (LDH) is an enzyme that catalyzes the interconversion of pyruvate and lactate along with the conversion of NADH to NAD+. LDH is known by other names such as lactic acid dehydrogenase and LDH-5. The enzyme has a molecular weight of approximately 36 kDa. LDH exists in almost all tissues having multiple isoforms that are expressed differently depending on the tissue type. It shows high expression in muscle tissue liver and heart indicating its extensive role in energy metabolism.

Biological function summary

Lactate dehydrogenase plays a critical role in anaerobic glycolysis. The enzyme helps in regenerating NAD+ from NADH allowing glycolysis to continue in the absence of oxygen. LDH is not a part of any larger protein complex working independently to fulfill its function in the glycolytic pathway. It serves in rapid energy production especially under hypoxic or exertional conditions where oxygen supply is limited.

Pathways

LDH is significantly involved in the glycolysis and gluconeogenesis pathways. Within glycolysis LDH helps facilitate the conversion of pyruvate to lactate during anaerobic conditions a step important for ATP production when oxygen is scarce. The enzyme is tied closely to phosphofructokinase-1 (PFK-1) in glycolysis given that both enzymes are central to maintaining the glycolytic flow. In gluconeogenesis though functionally reversed from its role in glycolysis LDH helps to manage lactate removal an important step for glucose synthesis from non-carbohydrate sources.

Lactate dehydrogenase levels often act as a biomarker for tissue damage or certain cancers as its release into the bloodstream signals cellular injury or death. Elevated LDH levels are associated with conditions like myocardial infarction and certain forms of anemia. In cancer such as lymphoma or leukemia LDH correlates with the progression of the disease and acts as a prognostic marker. LDH's connection to these conditions often leads to insights into disease severity and progression due to its association with proteins like p53 and HIF-1 which play roles in cellular metabolism and hypoxia response.

style

left-column

일반 정보

기능

Interconverts simultaneously and stereospecifically pyruvate and lactate with concomitant interconversion of NADH and NAD(+).

서열 유사성

Belongs to the LDH/MDH superfamily. LDH family.

Post-translational modifications

ISGylated.

style

left-column

제품 프로토콜

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

Interconverts simultaneously and stereospecifically pyruvate and lactate with concomitant interconversion of NADH and NAD(+).

See full target information LDHA

대체 명칭 보기

PIG19, LDHA, L-lactate dehydrogenase A chain, LDH-A, Cell proliferation-inducing gene 19 protein, LDH muscle subunit, Renal carcinoma antigen NY-REN-59, LDH-M

style

left-column

제품이 사용된 논문 (5)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cell death & disease 15:64 PubMed38233415

2024

FKBP10 promotes clear cell renal cell carcinoma progression and regulates sensitivity to the HIF2α blockade by facilitating LDHA phosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Ren Liu,Zhihao Zou,Lingwu Chen,Yuanfa Feng,Jianheng Ye,Yulin Deng,Xuejin Zhu,Yixun Zhang,Jundong Lin,Shanghua Cai,Zhenfeng Tang,Yingke Liang,Jianming Lu,Yangjia Zhuo,Zhaodong Han,Xiaohui Ling,Yuxiang Liang,Zongren Wang,Weide Zhong

ACS sensors 6:842-851 PubMed33724791

2021

Multiplexed Microfluidic Cartridge for At-Line Protein Monitoring in Mammalian Cell Culture Processes for Biopharmaceutical Production.

Applications

Unspecified application

Species

Unspecified reactive species

Inês F Pinto,Ruben R G Soares,Meeri E-L Mäkinen,Veronique Chotteau,Aman Russom

Molecules (Basel, Switzerland) 25: PubMed32121469

2020

Chiral Pyridine-3,5-bis- (L-phenylalaninyl-L-leucinyl) Schiff Base Peptides as Potential Anticancer Agents: Design, Synthesis, and Molecular Docking Studies Targeting Lactate Dehydrogenase-A.

Applications

Unspecified application

Species

Unspecified reactive species

Abd El-Galil E Amr,Randa E Abdel Mageid,Mohamed El-Naggar,Ahmed M Naglah,Eman S Nossier,Elsayed A Elsayed

Molecular & cellular proteomics : MCP 18:1330-1344 PubMed31010818

2019

Cathepsin L Regulates Metabolic Networks Controlling Rapid Cell Growth and Proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Tommy Weiss-Sadan,Gal Itzhak,Farnusch Kaschani,Zhanru Yu,Mohamed Mahameed,Adi Anaki,Yael Ben-Nun,Emmanuelle Merquiol,Boaz Tirosh,Benedikt Kessler,Markus Kaiser,Galia Blum

Nature chemical biology 13:494-500 PubMed28263965

2017

L-2-Hydroxyglutarate production arises from noncanonical enzyme function at acidic pH.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew M Intlekofer,Bo Wang,Hui Liu,Hardik Shah,Carlos Carmona-Fontaine,Ariën S Rustenburg,Salah Salah,M R Gunner,John D Chodera,Justin R Cross,Craig B Thompson

제품이 사용된 논문 모두 보기

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Recombinant human Lactate Dehydrogenase protein (Active) (His tag N-Terminus)

Western blot - Recombinant human Lactate Dehydrogenase protein (Active) (His tag N-Terminus) (AB93699)

SDS-PAGE - Recombinant human Lactate Dehydrogenase protein (Active) (His tag N-Terminus) (AB93699)

주요 정보

Purity

발현 시스템

Tags

Applications

Biologically active

Biological activity

Accession

Animal free

Carrier free

Species

보관 버퍼

보완 제품

Reagents, Controls & Accessories

Proteins & Peptides

Assay Kits

Proteins & Peptides

Primary Antibodies

Reactivity 정보

제품 세부 정보

서열 정보

특성 및 보관 정보

제형

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

일반 정보

기능

서열 유사성

Post-translational modifications

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (5)

Product promise