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AB322747

Recombinant Human MDH1 Protein Standard (His tag)

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Recombinant Human MDH1 Protein Standard (His tag) is a Human Full Length protein, expressed in Escherichia coli, with >80%, suitable for SDS-PAGE, sELISA.
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Sandwich ELISA - Recombinant Human MDH1 Protein Standard (His tag) (AB322747)
  • sELISA

Supplier Data

Sandwich ELISA - Recombinant Human MDH1 Protein Standard (His tag) (AB322747)

Sandwich ELISA with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

SDS-PAGE - Recombinant Human MDH1 Protein Standard (His tag) (AB322747)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human MDH1 Protein Standard (His tag) (AB322747)

SDS-PAGE analysis of ab322747 under reducing conditions for 2ug protein.

주요 정보

Purity

>80% SDS-PAGE

발현 시스템

Escherichia coli

Tags

His tag C-Terminus

Applications

sELISA, SDS-PAGE

applications

Biologically active

No

Accession

P40925-1

Animal free

Yes

Carrier free

No

Species

Human

보관 버퍼

pH: 7.3 - 7.5 Constituents: 2.922% Sodium chloride, 0.64107% disodium;hydrogen phosphate;dodecahydrate, 0.02858% Potassium phosphate monobasic

storage-buffer

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보완 제품

Reagents, Controls & Accessories

AB313995

Prestained Protein Ladder - Mid-range molecular weight (15 - 170 kDa)

SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (15 - 170 kDa) (AB313995)

  • 0
  • 0
Proteins & Peptides

AB99238

Recombinant human MDH2 protein (His tag N-Terminus)

SDS-PAGE - Recombinant human MDH2 protein (His tag N-Terminus) (AB99238)

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Proteins & Peptides

AB283432

Recombinant Human PSAP protein

SDS-PAGE - Recombinant Human PSAP protein (AB283432)

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Proteins & Peptides

AB96765

Recombinant human Lactate Dehydrogenase B/LDH-B protein (His tag N-Terminus)

SDS-PAGE - Recombinant human Lactate Dehydrogenase B/LDH-B protein (His tag N-Terminus) (AB96765)

  • 0
  • 0
Proteins & Peptides

AB316456

Recombinant Human CHIT1 Protein Standard (His tag)

Sandwich ELISA - Recombinant Human CHIT1 Protein Standard (His tag) (AB316456)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.

Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.

The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Supportteam to know which antibody pair is suitable for this protein.

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서열 정보

[{"linker":null,"sequence":null,"proteinLength":"Full Length","predictedMolecularWeight":"37.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P40925","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification His Tag
배송 시 보관 조건
Dry Ice
적절한 단기 보관 조건
-80°C
적절한 장기 보관 조건
-80°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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일반 정보

기능

Catalyzes the reduction of aromatic alpha-keto acids in the presence of NADH (PubMed : 2449162, PubMed : 3052244). Plays essential roles in the malate-aspartate shuttle and the tricarboxylic acid cycle, important in mitochondrial NADH supply for oxidative phosphorylation (PubMed : 31538237). Catalyzes the reduction of 2-oxoglutarate to 2-hydroxyglutarate, leading to elevated reactive oxygen species (ROS) (PubMed : 34012073).

서열 유사성

Belongs to the LDH/MDH superfamily. MDH type 2 family.

Post-translational modifications

ISGylated.. Acetylation at Lys-118 dramatically enhances enzymatic activity and promotes adipogenic differentiation.

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제품 프로토콜

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타겟 정보

Catalyzes the reduction of aromatic alpha-keto acids in the presence of NADH (PubMed : 2449162, PubMed : 3052244). Plays essential roles in the malate-aspartate shuttle and the tricarboxylic acid cycle, important in mitochondrial NADH supply for oxidative phosphorylation (PubMed : 31538237). Catalyzes the reduction of 2-oxoglutarate to 2-hydroxyglutarate, leading to elevated reactive oxygen species (ROS) (PubMed : 34012073).
See full target information MDH1

대체 명칭 보기

MDHA, MDH1, Aromatic alpha-keto acid reductase, Cytosolic malate dehydrogenase, KAR
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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