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AB238230

Recombinant Human ME3 protein (His tag)

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Recombinant Human ME3 protein (His tag) is a Human Fragment protein, in the 24 to 590 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

대체 명칭 보기

NADP-ME, Malic enzyme 3, ME3

1 이미지
SDS-PAGE - Recombinant Human ME3 protein (His tag) (AB238230)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human ME3 protein (His tag) (AB238230)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel analysis of ab238230.

주요 정보

Purity

>85% SDS-PAGE

발현 시스템

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 7.2 - 7.4 Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

storage-buffer

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보완 제품

Proteins & Peptides

AB211318

Recombinant human NME3 protein (His tag N-Terminus)

SDS-PAGE - Recombinant human NME3 protein (His tag N-Terminus) (AB211318)

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Primary Antibodies

AB172972

Anti-ME3 antibody [EPR10378]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ME3 antibody [EPR10378] (AB172972)

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Proteins & Peptides

AB152518

Recombinant Human ME1 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human ME1 protein (GST tag N-Terminus) (AB152518)

  • 0
  • 0
Primary Antibodies

AB97445

Anti-ME1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-ME1 antibody (AB97445)

  • 4
  • 1
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"RWTPTAPAQGCHSKPGPARPVPLKKRGYDVTRNPHLNKGMAFTLEERLQLGIHGLIPPCFLSQDVQLLRIMRYYERQQSDLDKYIILMTLQDRNEKLFYRVLTSDVEKFMPIVYTPTVGLACQHYGLTFRRPRGLFITIHDKGHLATMLNSWPEDNIKAVVVTDGERILGLGDLGCYGMGIPVGKLALYTACGGVNPQQCLPVLLDVGTNNEELLRDPLYIGLKHQRVHGKAYDDLLDEFMQAVTDKFGINCLIQFEDFANANAFRLLNKYRNKYCMFNDDIQGTASVAVAGILAALRITKNKLSNHVFVFQGAGEAAMGIAHLLVMALEKEGVPKAEATRKIWMVDSKGLIVKGRSHLNHEKEMFAQDHPEVNSLEEVVRLVKPTAIIGVAAIAGAFTEQILRDMASFHERPIIFALSNPTSKAECTAEKCYRVTEGRGIFASGSPFKSVTLEDGKTFIPGQGNNAYVFPGVALGVIAGGIRHIPDEIFLLTAEQIAQEVSEQHLSQGRLYPPLSTIRDVSLRIAIKVLDYAYKHNLASYYPEPKDKEAFVRSLVYTPDYDSFTLD","proteinLength":"Fragment","predictedMolecularWeight":"67.2 kDa","actualMolecularWeight":null,"aminoAcidEnd":590,"aminoAcidStart":24,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q16798","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
보관 정보
Avoid freeze / thaw cycle
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

ME3 also known as malic enzyme 3 or mitochondrial NADP+-dependent malic enzyme plays an important role in cellular metabolism. It catalyzes the oxidative decarboxylation of malate to pyruvate generating NADPH in the process. The protein has a molecular mass of approximately 65 kDa. ME3 is mainly expressed in tissues with high metabolic demands such as the liver and adipose tissue where it supports various biosynthetic pathways.
Biological function summary

ME3 influences cellular redox balance via its production of NADPH an important reducing agent. It is not part of a larger protein complex but serves an essential function on its own. ME3 contributes to lipid biosynthesis by supplying NADPH for fatty acid elongation and cholesterol synthesis which supports cell growth and proliferation. Its activity impacts overall metabolic homeostasis.

Pathways

ME3 has a significant role in the tricarboxylic acid (TCA) cycle and lipid metabolism. It interfaces with the TCA cycle by providing a critical link between carbohydrate and lipid metabolism through pyruvate production. ME3 interacts with other metabolic enzymes like citrate synthase emphasizing its integrating function in energy production and storage pathways.

ME3's dysregulation associates with metabolic conditions such as obesity and non-alcoholic fatty liver disease (NAFLD). Altered ME3 activity can impact fatty acid metabolism and contribute to lipid accumulation in liver cells. It also interacts with proteins like acetyl-CoA carboxylase involved in fatty acid biosynthesis highlighting its potential role in disease progression. Understanding ME3's function offers insights into the etiology of these metabolic disorders and opportunities for therapeutic intervention.
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일반 정보

기능

Catalyzes the oxidative decarboxylation of (S)-malate to pyruvate using NADP(+) as a cofactor (PubMed : 7818469). Can also reverse the decarboxylation reaction, but only with significantly lower efficiency (PubMed : 7818469).

서열 유사성

Belongs to the malic enzymes family.

Subcellular localisation

Mitochondrion matrix

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제품 프로토콜

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타겟 정보

Catalyzes the oxidative decarboxylation of (S)-malate to pyruvate using NADP(+) as a cofactor (PubMed : 7818469). Can also reverse the decarboxylation reaction, but only with significantly lower efficiency (PubMed : 7818469).
See full target information ME3
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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