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AB180327

Recombinant Human MED3 protein (denatured) (His tag N-Terminus)

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Recombinant Human MED3 protein (denatured) (His tag N-Terminus) is a Human Full Length protein, in the 1 to 311 aa range, expressed in Escherichia coli, with >80%, suitable for SDS-PAGE.

대체 명칭 보기

CRSP34, CRSP8, MED27, Mediator of RNA polymerase II transcription subunit 27, Cofactor required for Sp1 transcriptional activation subunit 8, Mediator complex subunit 27, P37 TRAP/SMCC/PC2 subunit, Transcriptional coactivator CRSP34, CRSP complex subunit 8

1 이미지
SDS-PAGE - Recombinant Human MED3 protein (denatured) (His tag N-Terminus) (AB180327)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human MED3 protein (denatured) (His tag N-Terminus) (AB180327)

15% SDS-PAGE analysis of 3μg ab180327.

주요 정보

Purity

>80% SDS-PAGE

발현 시스템

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris HCl

storage-buffer

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보완 제품

Proteins & Peptides

AB131673

Recombinant Human MED7 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human MED7 protein (His tag N-Terminus) (AB131673)

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Proteins & Peptides

AB152670

Recombinant Human Nav1.6/SCN8A protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human Nav1.6/SCN8A protein (GST tag N-Terminus) (AB152670)

  • 0
  • 0
Proteins & Peptides

AB162385

Recombinant Human MED31 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human MED31 protein (GST tag N-Terminus) (AB162385)

  • 0
  • 0
Proteins & Peptides

AB164823

Recombinant Human MED30 / TRAP25 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human MED30 / TRAP25 protein (GST tag N-Terminus) (AB164823)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

This product was previously labelled as CRSP8
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서열 정보

[{"linker":null,"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMADVINVSVNLEAFSQAISAIQALRSSVSRVFDCLKDGMRNKETLEGREKAFIAHFQDNLHSVNRDLNELERLSNLVGKPSENHPLHNSGLLSLDPVQDKTPLYSQLLQAYKWSNKLQYHAGLASGLLNQQSLKRSANQMGVSAKRRPKAQPTTLVLPPQYVDDVISRIDRMFPEMSIHLSRPNGTSAMLLVTLGKVLKVIVVMRSLFIDRTIVKGYNENVYTEDGKLDIWSKSNYQVFQKVTDHATTALLHYQLPQMPDVVVRSFMTWLRSYIKLFQAPCQRCGKFLQDGLPPTWRDFRTLEAFHDTCRQ","proteinLength":"Full Length","predictedMolecularWeight":"37.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":311,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q6P2C8","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The MED3 protein also known as Vitamin D3 receptor interacting protein complex 12 (DRIP12) possesses a mass of approximately 64 kDa. This protein acts as part of the larger Mediator complex which plays an important role in the regulation of gene transcription. MED3 is ubiquitously expressed in various tissues reflecting its essential function in gene expression. It modulates transcription by serving as a bridge between transcription factors and RNA polymerase II.
Biological function summary

Functions of MED3 extend to the facilitation of transcriptional activation processes. Being a part of the Mediator complex MED3 influences RNA polymerase II-dependent transcription in eukaryotic cells. It works closely with other core subunits to recruit the complex to gene promoters and enable efficient transcription initiation. MED3 by interacting with transcriptional regulators adjusts the transcriptional output necessary for cell growth and differentiation.

Pathways

Studies show that MED3 is involved in the regulation of Wnt signaling and the Notch signaling pathways. These pathways are key in cell fate determination and developmental processes. The MED3 protein intersects with the activities of other transcriptional regulators like beta-catenin in the Wnt pathway emphasizing its impact on important developmental gene expression.

MED3 holds an association with certain cancers and developmental disorders. Dysregulation of MED3 activity may contribute to colorectal cancer implicating its involvement with the aberrant activation of Wnt signaling. In some developmental disorders the improper function of MED3 can disrupt normal transcriptional regulation also impacting Notch signaling-related proteins. Understanding MED3's role in these contexts provides insights into its potential as a therapeutic target.
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일반 정보

기능

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

서열 유사성

Belongs to the Mediator complex subunit 27 family.

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제품 프로토콜

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타겟 정보

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
See full target information MED27
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Molecular and cellular biology 39: PubMed30602494

2019

RNA Binding Protein HuR Promotes Autophagosome Formation by Regulating Expression of Autophagy-Related Proteins 5, 12, and 16 in Human Hepatocellular Carcinoma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Eunbyul Ji,Chongtae Kim,Hoin Kang,Sojin Ahn,Myeongwoo Jung,Youlim Hong,Hyosun Tak,Sukchan Lee,Wook Kim,Eun Kyung Lee
제품이 사용된 논문 모두 보기
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Product promise

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