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AB105578

Recombinant Human MEK3 protein (His tag N-Terminus)

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(1 출판물)

Recombinant Human MEK3 protein (His tag N-Terminus) is a Human Fragment protein, in the 20 to 347 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

대체 명칭 보기

MEK3, MKK3, PRKMK3, SKK2, MAP2K3, Dual specificity mitogen-activated protein kinase kinase 3, MAP kinase kinase 3, MAPKK 3, MAPK/ERK kinase 3, Stress-activated protein kinase kinase 2, MEK 3, SAPK kinase 2, SAPKK-2, SAPKK2

1 이미지
SDS-PAGE - Recombinant Human MEK3 protein (His tag N-Terminus) (AB105578)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human MEK3 protein (His tag N-Terminus) (AB105578)

15% SDS-PAGE analysis of ab105578 (3ug)

주요 정보

Purity

>90% SDS-PAGE

ab105578 was purified using conventional chromatography.

발현 시스템

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl

storage-buffer

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보완 제품

Primary Antibodies

AB195037

Anti-MEK3 antibody [EPR17345-104]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK3 antibody [EPR17345-104] (AB195037)

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Proteins & Peptides

AB269204

Recombinant Human Neurotrophin 3 protein

SDS-PAGE - Recombinant Human Neurotrophin 3 protein (AB269204)

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Primary Antibodies

AB300093

Alexa Fluor® 647 Anti-TNF-alpha antibody [EPR21753-109]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-TNF-alpha antibody [EPR21753-109] (AB300093)

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Primary Antibodies

AB283818

Anti-IL-1 beta antibody [RM1009]

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunocytochemistry - Anti-IL-1 beta antibody [RM1009] (AB283818)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"MGSSHHHHHHSSGLVPRGSHMSKPPAPNPTPPRNLDSRTFITIGDRNFEVEADDLVTISELGRGAYGVVEKVRHAQSGTIMAVKRIRATVNSQEQKRLLMDLDINMRTVDCFYTVTFYGALFREGDVWICMELMDTSLDKFYRKVLDKNMTIPEDILGEIAVSIVRALEHLHSKLSVIHRDVKPSNVLINKEGHVKMCDFGISGYLVDSVAKTMDAGCKPYMAPERINPELNQKGYNVKSDVWSLGITMIEMAILRFPYESWGTPFQQLKQVVEEPSPQLPADRFSPEFVDFTAQCLRKNPAERMSYLELMEHPFFTLHKTKKTDIAAFVKEILGEDS","proteinLength":"Fragment","predictedMolecularWeight":"38.3 kDa","actualMolecularWeight":null,"aminoAcidEnd":347,"aminoAcidStart":20,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P46734","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

MEK3 also known as MAP2K3 is a kinase that plays a significant mechanical role within cells. It is part of the MAP kinase family sharing functions with enzymes like MEK6. MEK3 has a molecular mass of approximately 38 kDa and is localized in cytoplasmic regions of various cell types. This protein is expressed in a wide range of tissues indicating its diverse functional roles across different biological systems.
Biological function summary

MEK3 is involved in the phosphorylation cascade within the mitogen-activated protein kinase (MAPK) pathway. It often exists as part of a complex working alongside other kinases to activate p38 MAPK which then regulates gene expression. The involvement of MEK3 in this signaling cascade influences processes such as cellular stress response and inflammation. Its ability to modulate these processes makes MEK3 an important component in maintaining cellular homeostasis.

Pathways

MEK3 functions as an essential link within the stress-activated pathways like the MAPK signaling pathway. It phosphorylates and activates the p38 MAPK pathway therefore regulating activities related to cellular stress and inflammatory responses. MEK3 works in conjunction with related kinases such as MEK6 to propagate the MAPK signaling cascade further emphasizing its important role in these pathways.

MEK3 has a documented involvement in inflammatory conditions and cancer. Its role in activating the p38 MAPK pathway connects it to inflammatory diseases where dysregulated signaling may lead to excessive inflammation. Furthermore aberrant MEK3 activity is associated with certain cancers as it affects cell proliferation and survival. MEK3's interactions with other kinases like MEK6 underline its involvement in these pathological processes making it a potential target for therapeutic intervention.
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일반 정보

기능

Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14.

서열 유사성

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.

Post-translational modifications

Autophosphorylated. Phosphorylation on Ser-218 and Thr-222 by MAP kinase kinase kinases positively regulates the kinase activity (PubMed:8622669). Phosphorylated by TAOK2 (PubMed:11279118).. (Microbial infection) Yersinia YopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway.

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제품 프로토콜

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타겟 정보

Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14.
See full target information MAP2K3
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cell death & disease 11:305 PubMed32366830

2020

Tumor suppressor death-associated protein kinase 1 inhibits necroptosis by p38 MAPK activation.

Applications

Unspecified application

Species

Unspecified reactive species

Yung-Hsuan Wu,Ting-Fang Chou,Leslie Young,Fu-Yi Hsieh,Hsuan-Yin Pan,Shu-Ting Mo,Shani Bialik Brown,Ruey-Hwa Chen,Adi Kimchi,Ming-Zong Lai
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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