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AB307786

Recombinant Human MICA Protein (His-tag) (Active)

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Recombinant Human MICA Protein (His-tag) (Active) is a Human Fragment protein, in the 24 to 308 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for HPLC, SDS-PAGE, Mass Spec, FuncS.

대체 명칭 보기

PERB11.1, MICA, MHC class I polypeptide-related sequence A, MIC-A

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Functional Studies - Recombinant Human MICA Protein (His-tag) (Active) (AB307786)
  • FuncS

Lab

Functional Studies - Recombinant Human MICA Protein (His-tag) (Active) (AB307786)

Loaded Human NKG2D, Fc Tag on Protein A Biosensor can bind Human MICA, His Tag with an affinity constant of 141 nM as determined in BLI assay (GatorBio Prime).

Mass Spectrometry - Recombinant Human MICA Protein (His-tag) (Active) (AB307786)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant Human MICA Protein (His-tag) (Active) (AB307786)

Mass determination by ESI-TOF. Predicted MW is 34341.59 Da (+/-10 Da by ESI-TOF). Observed MW is 34348.97 Da.

SDS-PAGE - Recombinant Human MICA Protein (His-tag) (Active) (AB307786)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human MICA Protein (His-tag) (Active) (AB307786)

SDS-PAGE analysis of ab307786

HPLC - Recombinant Human MICA Protein (His-tag) (Active) (AB307786)
  • HPLC

Supplier Data

HPLC - Recombinant Human MICA Protein (His-tag) (Active) (AB307786)

HPLC analysis of ab307786

주요 정보

Purity

>95% HPLC

SDS-PAGE >= 95%

Endotoxin 레벨

<0.005 EU/µg

발현 시스템

HEK 293 cells

Tags

His tag C-Terminus

Applications

Mass Spec, HPLC, FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Loaded Human NKG2D, Fc Tag on Protein A Biosensor can bind Human MICA, His Tag with an affinity constant of 141 nM as determined in BLI assay (GatorBio Prime).

Accession

Animal free

Yes

Carrier free

No

Species

Human

Reconstitution

Reconstitute in PBS

보관 버퍼

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

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보완 제품

Reagents, Controls & Accessories

AB325995

Prestained Protein Ladder - Broad molecular weight (11-250 kDa, 5-color, 11 bands)

SDS-PAGE - Prestained Protein Ladder - Broad molecular weight (11-250 kDa, 5-color, 11 bands) (AB325995)

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Assay Kits

AB59569

Human MICA ELISA Kit

ELISA - Human MICA ELISA Kit (AB59569)

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Primary Antibodies

AB224702

Anti-MICA + MICB antibody [EPR22031]

20ul selling size|KO Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MICA + MICB antibody [EPR22031] (AB224702)

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Proteins & Peptides

AB152060

Recombinant Human CD98 protein

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Proteins & Peptides

AB80350

Recombinant human HDAC9 protein (His tag C-Terminus)

Functional Studies - Recombinant human HDAC9 protein (His tag C-Terminus) (AB80350)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"EPHSLRYNLTVLSWDGSVQSGFLTEVHLDGQPFLRCDRQKCRAKPQGQWAEDVLGNKTWDRETRDLTGNGKDLRMTLAHIKDQKEGLHSLQEIRVCEIHEDNSTRSSQHFYYDGELFLSQNLETKEWTMPQSSRAQTLAMNVRNFLKEDAMKTKTHYHAMHADCLQELRRYLKSGVVLRRTVPPMVNVTRSEASEGNITVTCRASGFYPWNITLSWRQDGVSLSHDTQQWGDVLPDGNGTYQTWVATRICQGEEQRFTCYMEHSGNHSTHPVPSGKVLVLQSHWQ","proteinLength":"Fragment","predictedMolecularWeight":"34.34 kDa","actualMolecularWeight":"34.35 kDa","aminoAcidEnd":308,"aminoAcidStart":24,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"Q29983","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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특성 및 보관 정보

제형
Lyophilized
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
Ambient
적절한 장기 보관 조건
Ambient
True
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

MICA also known as MHC class I polypeptide-related sequence A is a protein encoded by the MICA gene. It has a molecular weight of approximately 62 kDa. MICA is a stress-induced ligand expressed on the surface of various cell types including epithelial cells fibroblasts and some tumor cells. This protein is part of the MHC class I chain-related (MIC) family which plays an important role in the immune system by interacting with natural killer (NK) cells and certain T cells.
Biological function summary

The MICA protein acts as a ligand for the NKG2D receptor an activating receptor found on NK cells and some T-cell subsets. MICA does not require expression as part of a complex with β2-microglobulin for function. When cells express MICA they signal distress allowing immune cells to target and destroy potentially harmful cells such as those infected with viruses or transformed into cancer cells. This interaction is an important component of the immune surveillance system.

Pathways

MICA functions within the natural cytotoxicity effector pathway heavily involved in immune responses. It is also relevant in the DNA damage response pathways. Within these pathways MICA interacts with proteins like NKG2D and plays a part in mediating cellular responses to stress and damage. These interactions enhance the capacity of immune cells to recognize and eliminate abnormal cells.

MICA is associated with conditions such as psoriasis and certain types of cancer. Studies have shown increased levels of soluble MICA in the sera of patients with psoriasis indicating a role in inflammation. Additionally in cancer MICA expression affects tumor immunity connecting to the NKG2D receptor to influence immune cell targeting and destruction of tumor cells. Researchers use tools such as MICA ELISA kits and imaging techniques to study its role in these diseases.
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일반 정보

기능

Widely expressed membrane-bound protein which acts as a ligand to stimulate an activating receptor KLRK1/NKG2D, expressed on the surface of essentially all human natural killer (NK), gammadelta T and CD8 alphabeta T-cells (PubMed : 11491531, PubMed : 11777960). Up-regulated in stressed conditions, such as viral and bacterial infections or DNA damage response, serves as signal of cellular stress, and engagement of KLRK1/NKG2D by MICA triggers NK-cells resulting in a range of immune effector functions, such as cytotoxicity and cytokine production (PubMed : 10426993).

서열 유사성

Belongs to the MHC class I family. MIC subfamily.

Post-translational modifications

N-glycosylated. Glycosylation is not essential for interaction with KLRK1/NKG2D but enhances complex formation.. Proteolytically cleaved and released from the cell surface of tumor cells which impairs KLRK1/NKG2D expression and T-cell activation.. Palmitoylated on cysteine residues in the cytoplasmic tail leading to its association with membrane microdomains enriched in cholesterol.. N-glycosylation is necessary for cell surface expression.. (Microbial infection) Ubiquitinated by human herpesvirus 8 protein K5, leading to degradation.

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제품 프로토콜

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타겟 정보

Widely expressed membrane-bound protein which acts as a ligand to stimulate an activating receptor KLRK1/NKG2D, expressed on the surface of essentially all human natural killer (NK), gammadelta T and CD8 alphabeta T-cells (PubMed : 11491531, PubMed : 11777960). Up-regulated in stressed conditions, such as viral and bacterial infections or DNA damage response, serves as signal of cellular stress, and engagement of KLRK1/NKG2D by MICA triggers NK-cells resulting in a range of immune effector functions, such as cytotoxicity and cytokine production (PubMed : 10426993).
See full target information MICA
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Product promise

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