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AB307787

Recombinant Human MICB Protein (His-tag) (Active)

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Recombinant Human MICB Protein (His-tag) (Active) is a Human Fragment protein, in the 23 to 309 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for HPLC, SDS-PAGE, Mass Spec, FuncS.

대체 명칭 보기

PERB11.2, MICB, MHC class I polypeptide-related sequence B, MIC-B

4 이미지
Functional Studies - Recombinant Human MICB Protein (His-tag) (Active) (AB307787)
  • FuncS

Lab

Functional Studies - Recombinant Human MICB Protein (His-tag) (Active) (AB307787)

Loaded Human NKG2D, Fc Tag on Protein A Biosensor can bind Human MICB, His Tag with an affinity constant of 698 nM as determined in BLI assay (GatorBio Prime).

Mass Spectrometry - Recombinant Human MICB Protein (His-tag) (Active) (AB307787)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant Human MICB Protein (His-tag) (Active) (AB307787)

Mass determination by ESI-TOF. Predicted MW is 34197.21 Da (+/- 10 Da by ESI-TOF). Observed MW is 34202.22 Da.

HPLC - Recombinant Human MICB Protein (His-tag) (Active) (AB307787)
  • HPLC

Supplier Data

HPLC - Recombinant Human MICB Protein (His-tag) (Active) (AB307787)

HPLC analysis of ab307787

SDS-PAGE - Recombinant Human MICB Protein (His-tag) (Active) (AB307787)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human MICB Protein (His-tag) (Active) (AB307787)

SDS-PAGE analysis of ab307787

주요 정보

Purity

>95% HPLC

SDS-PAGE >= 95%

Endotoxin 레벨

<0.005 EU/µg

발현 시스템

HEK 293 cells

Tags

His tag C-Terminus

Applications

Mass Spec, HPLC, FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Loaded Human NKG2D, Fc Tag on Protein A Biosensor can bind Human MICB, His Tag with an affinity constant of 698 nM as determined in BLI assay (GatorBio Prime).

Accession

Animal free

Yes

Carrier free

No

Species

Human

Reconstitution

Reconstitute in PBS

보관 버퍼

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

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보완 제품

Primary Antibodies

AB241513

Anti-MICA + MICB antibody [EPR22031] - BSA and Azide free

KO Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MICA + MICB antibody [EPR22031] - BSA and Azide free (AB241513)

  • 1
  • 1
Proteins & Peptides

AB307791

Recombinant Human CD134 / OX40L receptor Protein (His-tag) (Active)

Premium Bioactive

Functional Studies - Recombinant Human CD134 / OX40L receptor Protein (His-tag) (Active) (AB307791)

  • 0
  • 0
Assay Kits

AB267804

Human MICB ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human MICB ELISA Kit (AB267804)

  • 0
  • 0
Proteins & Peptides

AB151344

Recombinant Human INHBC protein

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"AEPHSLRYNLMVLSQDGSVQSGFLAEGHLDGQPFLRYDRQKRRAKPQGQWAENVLGAKTWDTETEDLTENGQDLRRTLTHIKDQKGGLHSLQEIRVCEIHEDSSTRGSRHFYYDGELFLSQNLETQESTVPQSSRAQTLAMNVTNFWKEDAMKTKTHYRAMQADCLQKLQRYLKSGVAIRRTVPPMVNVTCSEVSEGNITVTCRASSFYPRNITLTWRQDGVSLSHNTQQWGDVLPDGNGTYQTWVATRIRQGEEQRFTCYMEHSGNHGTHPVPSGKALVLQSQRTD","proteinLength":"Fragment","predictedMolecularWeight":"34.2 kDa","actualMolecularWeight":"34.2 kDa","aminoAcidEnd":309,"aminoAcidStart":23,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"Q29980","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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특성 및 보관 정보

제형
Lyophilized
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
Ambient
적절한 장기 보관 조건
Ambient
True
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

MICB also known as MHC class I polypeptide-related sequence B is a protein with a molecular weight of around 42 kDa. It anchors on cell surfaces and is widely expressed in epithelial cells fibroblasts and monocytes. MICB surfaces on stressed cells but remains minimal in normal conditions indicating its involvement in cell stress response. It is an activating ligand for NKG2D receptor on natural killer (NK) cells and certain T cells playing a major role in immune surveillance.
Biological function summary

MICB contributes to immune system functioning by presenting stress-induced antigens to immune cells. It does not belong to a complex but engages in interactions critical for activating cytotoxic responses. By binding to the NKG2D receptor it helps activate NK cells and certain T cell subsets which results in the elimination of damaged or transformed cells. This immune activation is important for the surveillance against cancer and infected cells maintaining tissue homeostasis.

Pathways

MICB plays a role in the stress-induced NKG2D signaling pathway and is involved in the immune response pathway. It cooperates with proteins such as NKG2D and MICA another ligand of the NKG2D receptor to initiate immune cell-mediated cytotoxicity. These pathways activate NK cells and CD8+ T cells important for removing stressed or altered cells thereby influencing the body's defense mechanisms.

MICB has connections to cancer and autoimmune disorders. Aberrant expression of MICB appears in tumors affecting tumor immune evasion and associates with autoimmune diseases influencing immune tolerance. Dysregulation of MICB activity can affect interactions with proteins like NKG2D altering immune responses and contributing to disease progression. Therefore understanding MICB's role is critical for developing therapeutic interventions targeting immune-related diseases.
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일반 정보

기능

Widely expressed membrane-bound protein which acts as a ligand to stimulate an activating receptor KLRK1/NKG2D, expressed on the surface of essentially all human natural killer (NK), gammadelta T and CD8+ alphabeta T-cells (PubMed : 11491531, PubMed : 11777960). Up-regulated in stressed conditions, such as viral and bacterial infections or DNA damage response, serves as signal of cellular stress, and engagement of KLRK1/NKG2D by MICA triggers NK-cells resulting in a range of immune effector functions, such as cytotoxicity and cytokine production.

서열 유사성

Belongs to the MHC class I family. MIC subfamily.

Post-translational modifications

Proteolytically cleaved and released from the cell surface of tumor cells.

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제품 프로토콜

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타겟 정보

Widely expressed membrane-bound protein which acts as a ligand to stimulate an activating receptor KLRK1/NKG2D, expressed on the surface of essentially all human natural killer (NK), gammadelta T and CD8+ alphabeta T-cells (PubMed : 11491531, PubMed : 11777960). Up-regulated in stressed conditions, such as viral and bacterial infections or DNA damage response, serves as signal of cellular stress, and engagement of KLRK1/NKG2D by MICA triggers NK-cells resulting in a range of immune effector functions, such as cytotoxicity and cytokine production.
See full target information MICB
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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