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AB134527

Recombinant Human MOCS2 protein (DDDDK tag N-Terminus + His tag N-Terminus)

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Recombinant Human MOCS2 protein (DDDDK tag N-Terminus + His tag N-Terminus) is a Human Full Length protein, in the 1 to 188 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

대체 명칭 보기

MCBPE, MOCO1, MOCS2, Molybdopterin synthase catalytic subunit, MOCO1-B, Molybdenum cofactor synthesis protein 2 large subunit, Molybdenum cofactor synthesis protein 2B, Molybdopterin-synthase large subunit, MOCS2B, MPT synthase large subunit

1 이미지
SDS-PAGE - Recombinant Human MOCS2 protein (DDDDK tag N-Terminus + His tag N-Terminus) (AB134527)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human MOCS2 protein (DDDDK tag N-Terminus + His tag N-Terminus) (AB134527)

15% SDS-PAGE analysis of 3 μg ab134527.

주요 정보

Purity

>90% SDS-PAGE

ab134527 is purified using conventional chromatography techniques.

발현 시스템

Escherichia coli

Tags

DDDDK tag N-Terminus His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 8 Constituents: 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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보완 제품

Primary Antibodies

AB230013

Anti-MOCS2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-MOCS2 antibody (AB230013)

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Proteins & Peptides

AB139254

Recombinant Human CD3 zeta protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human CD3 zeta protein (His tag N-Terminus) (AB139254)

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Proteins & Peptides

AB152829

Recombinant Human BAT3/BAG-6 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human BAT3/BAG-6 protein (GST tag N-Terminus) (AB152829)

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Proteins & Peptides

AB307191

Recombinant Human PCNA protein (His tag)

Mass Spectrometry - Recombinant Human PCNA protein (His tag) (AB307191)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSMSSLEISSSCFSLETKLPLSPPLVEDSAFEPSRKDMDEVEEKSKDVINFTAEKLSVDEVSQLVISPLCGAISLFVGTTRNNFEGKKVISLEYEAYLPMAENEVRKICSDIRQKWPVKHIAVFHRLGLVPVSEASIIIAVSSAHRAASLEAVSYAIDTLKAKVPIWKKEIYEESSTWKGNKECFWASNS","proteinLength":"Full Length","predictedMolecularWeight":"25 kDa","actualMolecularWeight":null,"aminoAcidEnd":188,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O96007","tags":[{"tag":"DDDDK","terminus":"N-Terminus"},{"tag":"His","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

MOCS2 also known as molybdenum cofactor synthesis 2 plays a mechanical role in the biosynthesis of molybdenum cofactor. The protein has a molecular mass of approximately 20 kDa. MOCS2 is mainly expressed in the liver and kidneys where it contributes to various cellular processes. It consists of two isoforms A and B which play unique roles in facilitating the conversion of precursor molecules into active cofactors.
Biological function summary

The molybdenum cofactor synthesis process requires several components to function effectively. MOCS2 is part of the molybdenum cofactor synthesis complex which is essential for proper enzyme activity that utilizes the molybdenum cofactor. Enzymes requiring this cofactor include xanthine oxidase and aldehyde oxidase both of which are critical for metabolizing purines and aldehydes. The formation and regulation of this complex ensure efficient enzyme activity across different tissues.

Pathways

MOCS2 participates in the molybdenum cofactor biosynthesis pathway. It aligns with the sulfur transfer pathway where it interacts closely with MOCS1 another molybdenum cofactor synthesis protein. Both proteins coordinate to ensure sufficient cofactor production for catalytic reactions. These interactions allow for the proper function of enzymes like sulfite oxidase linking MOCS2 to essential metabolic processes.

Mutations or dysfunction in MOCS2 can lead to molybdenum cofactor deficiency a rare metabolic disorder. This disorder results in neurological symptoms due to the accumulation of toxic substances unmetabolized due to insufficient cofactor. MOCS2’s relationship with MOCS1 is significant as alterations in either can cause similar metabolic disruptions leading to the disease. Understanding MOCS2's role in these pathways provides insights into therapeutic strategies for affected individuals.
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일반 정보

기능

Catalytic subunit of the molybdopterin synthase complex, a complex that catalyzes the conversion of precursor Z into molybdopterin. Acts by mediating the incorporation of 2 sulfur atoms from thiocarboxylated MOCS2A into precursor Z to generate a dithiolene group (By similarity) (PubMed : 12732628, PubMed : 15073332, PubMed : 25709896). Together with MBIP, inhibits the activity of stress kinase EIF2AK2/PKR; this may suppress JNK activation and subsequent stress-responsive transcription, or suppress eIF2a phosphorylation to favor translation (PubMed : 26705305).

서열 유사성

Belongs to the MoaE family. MOCS2B subfamily.

Subcellular localisation

Nucleus

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제품 프로토콜

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타겟 정보

Catalytic subunit of the molybdopterin synthase complex, a complex that catalyzes the conversion of precursor Z into molybdopterin. Acts by mediating the incorporation of 2 sulfur atoms from thiocarboxylated MOCS2A into precursor Z to generate a dithiolene group (By similarity) (PubMed : 12732628, PubMed : 15073332, PubMed : 25709896). Together with MBIP, inhibits the activity of stress kinase EIF2AK2/PKR; this may suppress JNK activation and subsequent stress-responsive transcription, or suppress eIF2a phosphorylation to favor translation (PubMed : 26705305).
See full target information MOCS2
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Product promise

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