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AB151382

Recombinant Human NADK protein

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Recombinant Human NADK protein is a Human Fragment protein, in the 64 to 446 aa range, expressed in Escherichia coli, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, HPLC.

대체 명칭 보기

NAD kinase, Poly(P)/ATP NAD kinase, NADK

주요 정보

Purity

>95% SDS-PAGE

Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.Supplied as a 0.2 µM filtered solution.

Endotoxin 레벨

< 1 EU/µg

발현 시스템

Escherichia coli

Tags

His tag N-Terminus

Applications

HPLC, SDS-PAGE

applications

Biologically active

No

Accession

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 7.5 Constituents: 0.88% Sodium chloride, 0.79% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

서열 정보

[{"linker":null,"sequence":"MGSSHHHHHHSSGLVPRGSHMSLHGPCPVTTFGPKACVLQNPQTIMHIQDPASQRLTWNKSPKSVLVIKKMRDASLLQPFKELCTHLMEENMIVYVEKKVLEDPAIASDESFGAVKKKFCTFREDYDDISNQIDFIICLGGDGTLLYASSLFQGSVPPVMAFHLGSLGFLTPFSFENFQSQVTQVIEGNAAVVLRSRLKVRVVKELRGKKTAVHNGLGEKGSQAAGLDMDVGKQAMQYQVLNEVVIDRGPSSYLSN","proteinLength":"Fragment","predictedMolecularWeight":"28.2 kDa","actualMolecularWeight":null,"aminoAcidEnd":446,"aminoAcidStart":64,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O95544","tags":[{"tag":"His","terminus":"N-Terminus"}]}]

특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
Up to 6 months
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
False

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

NAD kinase also known as NADK is a critical enzyme responsible for phosphorylating nicotinamide adenine dinucleotide (NAD+) to form NADP+. NADK has an approximate molecular weight of 50 kDa. Its expression is widespread but it is especially abundant in pancreatic and liver tissues. This enzyme converts NAD+ to NADP+ which is important for maintaining the balance of NADP+/NADPH within cells impacting several biochemical processes.
Biological function summary

The conversion role played by NADK is essential for maintaining cellular redox status and for biosynthetic reactions that require NADPH such as fatty acid synthesis and detoxifying reactive oxygen species. NADK functions independently without being part of larger protein complexes. It facilitates key cellular processes by ensuring adequate levels of NADPH a necessary cofactor in various anabolic reactions and cellular defense mechanisms.

Pathways

NADK prominently features in the pentose phosphate pathway which is important for the production of NADPH. It interacts with enzymes like glucose-6-phosphate dehydrogenase (G6PD) which is also involved in producing NADPH through this pathway. Additionally NADK plays a role in the citric acid cycle where its activity supports energy metabolism by maintaining cellular NADP+/NADPH ratios.

Altered NADK activity links to conditions such as cancer and diabetes. Its role in regulating redox state and metabolic pathways means that dysregulation can lead to oxidative stress contributing to the pathogenesis of several types of cancer. Disrupted glucose metabolism in diabetes associates G6PD and NADK since both are pivotal in maintaining NADPH levels highlighting the enzyme's connection with metabolic diseases.

일반 정보

서열 유사성

Belongs to the NAD kinase family.

제품 프로토콜

Product promise

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