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AB289740

Recombinant Human NADPH oxidase 4 protein

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Recombinant Human NADPH oxidase 4 protein is a Human Full Length protein, in the 1 to 578 aa range, expressed in Cell free, with >85%, suitable for SDS-PAGE.
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SDS-PAGE - Recombinant Human NADPH oxidase 4 protein (AB289740)
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Supplier Data

SDS-PAGE - Recombinant Human NADPH oxidase 4 protein (AB289740)

SDS-PAGE analysis of ab289740

주요 정보

Purity

>85% SDS-PAGE

발현 시스템

Cell free

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q9NPH5-1

Animal free

Yes

Carrier free

No

Species

Human

보관 버퍼

pH: 7.4 - 8 Constituents: 6% Trehalose, 0.87% Sodium chloride, 0.24% Tris, 0.05% dodecyl 2-(trimethylazaniumyl)ethyl phosphate

storage-buffer

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보완 제품

Reagents, Controls & Accessories

AB133411

Low Fluorescence Western Membrane (PVDF)

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Proteins & Peptides

AB114183

Recombinant Human NOX2/gp91phox protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human NOX2/gp91phox protein (GST tag N-Terminus) (AB114183)

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Proteins & Peptides

AB289772

Recombinant Human NOX1 protein

SDS-PAGE - Recombinant Human NOX1 protein (AB289772)

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  • 0
Primary Antibodies

AB310337

Anti-NOX2/gp91phox antibody [EPR28415-13]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOX2/gp91phox antibody [EPR28415-13] (AB310337)

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Proteins & Peptides

AB179799

NADPH oxidase 4 peptide

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  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"MAVSWRSWLANEGVKHLCLFIWLSMNVLLFWKTFLLYNQGPEYHYLHQMLGLGLCLSRASASVLNLNCSLILLPMCRTLLAYLRGSQKVPSRRTRRLLDKSRTFHITCGVTICIFSGVHVAAHLVNALNFSVNYSEDFVELNAARYRDEDPRKLLFTTVPGLTGVCMVVVLFLMITASTYAIRVSNYDIFWYTHNLFFVFYMLLTLHVSGGLLKYQTNLDTHPPGCISLNRTSSQNISLPEYFSEHFHEPFPEGFSKPAEFTQHKFVKICMEEPRFQANFPQTWLWISGPLCLYCAERLYRYIRSNKPVTIISVMSHPSDVMEIRMVKENFKARPGQYITLHCPSVSALENHPFTLTMCPTETKATFGVHLKIVGDWTERFRDLLLPPSSQDSEILPFIQSRNYPKLYIDGPFGSPFEESLNYEVSLCVAGGIGVTPFASILNTLLDDWKPYKLRRLYFIWVCRDIQSFRWFADLLCMLHNKFWQENRPDYVNIQLYLSQTDGIQKIIGEKYHALNSRLFIGRPRWKLLFDEIAKYNRGKTVGVFCCGPNSLSKTLHKLSNQNNSYGTRFEYNKESFS","proteinLength":"Full Length","predictedMolecularWeight":"69.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":578,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Cell free","accessionNumber":"Q9NPH5","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Lyophilized
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

NADPH oxidase 4 also known as NOX4 is an enzyme that catalyzes the production of reactive oxygen species (ROS) by transferring electrons from NADPH to molecular oxygen. NOX4 has a molecular weight of approximately 67 kDa. It is expressed in various tissues including the kidneys lungs and heart with particularly high expression in vascular smooth muscle cells and endothelial cells. The protein serves as a homo- or heterodimer and is distinct due to its ability to produce hydrogen peroxide directly.
Biological function summary

NADPH oxidase 4 plays a role in cellular signaling and oxidative stress responses. The enzyme is part of the NADPH oxidase family which consists of several members each forming complexes with regulatory subunits such as p22phox NOXO1 or NOXA1. These complexes contribute to NOX4's stable and constitutive activity impacting cell growth differentiation and apoptosis processes. Its persistent activation suggests it modulates basal ROS levels acting as a control point in redox-dependent cell signaling.

Pathways

NOX4 is intricately involved in the TGF-beta signaling pathway and the regulation of endothelial nitric oxide synthase (eNOS) activity. The TGF-beta signaling involves NOX4 in fibrotic responses and cellular transformations often intersecting with other proteins like Smad3. In the nitric oxide signaling pathway interactions with eNOS affect the bioavailability of nitric oxide impacting vascular function. These relationships indicate NOX4's role in managing oxidative stress and maintaining vascular homeostasis through specific signaling cascades involving these proteins.

NADPH oxidase 4 contributes significantly to the pathogenesis of idiopathic pulmonary fibrosis and atherosclerosis. In idiopathic pulmonary fibrosis excessive ROS production by NOX4 leads to fibroblast differentiation and tissue remodeling. NOX4's involvement in atherosclerosis relates to overproduction of vascular ROS which affects endothelial function and promotes inflammatory processes. The protein's interaction with TGF-beta in fibrosis and eNOS in vascular disorders emphasizes its role in influencing disease progression through pathways linked to oxidative damage.
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제품 프로토콜

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타겟 정보

NADPH oxidase that catalyzes predominantly the reduction of oxygen to H2O2 (PubMed : 14966267, PubMed : 15356101, PubMed : 15927447, PubMed : 21343298, PubMed : 25062272). Can also catalyze to a smaller extent, the reduction of oxygen to superoxide (PubMed : 10869423, PubMed : 11032835, PubMed : 15155719, PubMed : 15572675, PubMed : 15927447, PubMed : 16019190, PubMed : 16179589, PubMed : 16230378, PubMed : 16324151, PubMed : 25062272). May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity (PubMed : 16019190). May regulate insulin signaling cascade (PubMed : 14966267). May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB (PubMed : 15356101, PubMed : 15572675). May produce superoxide in the nucleus and play a role in regulating gene expression upon cell stimulation (PubMed : 16324151). Promotes ferroptosis, reactive oxygen species production and reduced glutathione (GSH) levels by activating NLRP3 inflammasome activation and cytokine release (PubMed : 39909992).. Isoform 4. NADPH oxidase that catalyzes the generation of superoxide from molecular oxygen utilizing NADPH as an electron donor (PubMed : 15721269, PubMed : 23393389). Involved in redox signaling in vascular cells (PubMed : 23393389). Modulates the nuclear activation of ERK1/2 and the ELK1 transcription factor, and is capable of inducing nuclear DNA damage (PubMed : 23393389).. Isoform 3. Lacks superoxide-generating NADPH oxidase activity.
See full target information NOX4

대체 명칭 보기

RENOX, NOX4, NADPH oxidase 4, Kidney oxidase-1, Kidney superoxide-producing NADPH oxidase, Renal NAD(P)H-oxidase, KOX-1
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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