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AB87685

Recombinant human NDRG1 protein (His tag C-Terminus)

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Recombinant human NDRG1 protein (His tag C-Terminus) is a Human Full Length protein, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, FuncS.
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SDS-PAGE - Recombinant human NDRG1 protein (His tag C-Terminus) (AB87685)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human NDRG1 protein (His tag C-Terminus) (AB87685)

SDS-PAGE analysis of ab87685.

주요 정보

Purity

>95% SDS-PAGE

ab87685 is purified using conventional chromatography.

발현 시스템

Escherichia coli

Tags

His tag C-Terminus

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

The ED50 for this effect is 0.5 - 1.5 ng/ml. Measured in a cell proliferation assay using MCF7 cell. <p><strong>Activity Assay</strong></p> <ol> <li>Cell line: MCF7 (Human breast adenocarcinoma cell)</li> <li>Maintenance Condition: RPMI 1640 containing 10% FBS</li> <li>Assay medium: serum free RPMI 1640</li> <li>Cell density: 2 x 104 cells/well (96 well plate, final volume 100ul)</li> <li>Incubation time : 40 hrs (after sample treatment)</li> <li>Concentration range: 0.19ng/ml - 50ng/ml</li> <li>Detection method : BrdU assay</li> </ol>

Accession

Q92597-1

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.00174% PMSF

storage-buffer

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보완 제품

Assay Kits

AB253569

Human SGK1 Antibody Pair - BSA and Azide free

RabMAb|Recombinant

Sandwich ELISA - Human SGK1 Antibody Pair - BSA and Azide free (AB253569)

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Primary Antibodies

AB278702

Anti-NDRG1 (phospho T346) antibody [NDRG1T346-F5]

20ul selling size|Recombinant

Flow Cytometry - Anti-NDRG1 (phospho T346) antibody [NDRG1T346-F5] (AB278702)

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Proteins & Peptides

AB114727

Recombinant Human SCN2A protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human SCN2A protein (GST tag N-Terminus) (AB114727)

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Proteins & Peptides

AB241527

Recombinant Human Nucleolin protein (His tag)

Mass Spectrometry - Recombinant Human Nucleolin protein (His tag) (AB241527)

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  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"MSREMQDVDLAEVKPLVEKGETITGLLQEFDVQEQDIETLHGSVHVTLCGTPKGNRPVILTYHDIGMNHKTCYNPLFNYEDMQEITQHFAVCHVDAPGQQDGAASFPAGYMYPSMDQLAEMLPGVLQQFGLKSIIGMGTGAGAYILTRFALNNPEMVEGLVLINVNPCAEGWMDWAASKISGWTQALPDMVVSHLFGKEEMQSNVEVVHTYRQHIVNDMNPGNLHLFINAYNSRRDLEIERPMPGTHTVTLQCPALLVVGDSSPAVDAVVECNSKLDPTKTTLLKMADCGGLPQISQPAKLAEAFKYFVQGMGYMPSASMTRLMRSRTASGSSVTSLDGTRSRSHTSEGTRSRSHTSEGTRSRSHTSEGAHLDITPNSGAAGNSAGPKSMEVSCLEHHHHHH","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q92597","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
True
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

NDRG1 or N-Myc downstream-regulated gene 1 also known as Cap43 Drg-1 and RTP is a protein that plays key roles in various cellular processes. It has a molecular mass of approximately 43 kDa. NDRG1 is ubiquitously expressed with higher levels in the brain heart liver and kidney. The protein also exhibits a significant presence in cytoplasm but can translocate to the nucleus depending on the cellular context and stimuli.
Biological function summary

NDRG1 functions in cell growth regulation stress response and apoptosis influencing differentiation and potentially acting as a tumor suppressor. It does not directly form part of a larger protein complex but interacts with other proteins to mediate its effects. Its involvement in cellular homeostasis places it as a significant player in maintaining normal cell physiology.

Pathways

NDRG1 is involved in the hypoxia and stress response pathways regulating cellular responses to low oxygen and oxidative stress. It interacts with HIF-1α a critical regulator of the cellular response to hypoxia and also modulates PTEN and p53 proteins that are important for cell cycle control and apoptosis. These pathways and interactions reflect its function in cellular regulatory networks.

NDRG1 has associations with cancer and Charcot-Marie-Tooth disease type 4D. In cancer abnormal expression of NDRG1 has been linked to tumor progression and metastasis where it interacts with proteins like E-cadherin to influence cell adhesion and migration. In Charcot-Marie-Tooth disease mutations in the NDRG1 gene disrupt normal nerve function pointing to its importance in peripheral nervous system health.
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일반 정보

기능

Stress-responsive protein involved in hormone responses, cell growth, and differentiation. Acts as a tumor suppressor in many cell types. Necessary but not sufficient for p53/TP53-mediated caspase activation and apoptosis. Has a role in cell trafficking, notably of the Schwann cell, and is necessary for the maintenance and development of the peripheral nerve myelin sheath. Required for vesicular recycling of CDH1 and TF. May also function in lipid trafficking. Protects cells from spindle disruption damage. Functions in p53/TP53-dependent mitotic spindle checkpoint. Regulates microtubule dynamics and maintains euploidy.

서열 유사성

Belongs to the NDRG family.

Post-translational modifications

Under stress conditions, phosphorylated in the C-terminal on many serine and threonine residues. Phosphorylated in vitro by PKA. Phosphorylation enhanced by increased intracellular cAMP levels. Homocysteine induces dephosphorylation. Phosphorylation by SGK1 is cell cycle dependent.

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제품 프로토콜

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타겟 정보

Stress-responsive protein involved in hormone responses, cell growth, and differentiation. Acts as a tumor suppressor in many cell types. Necessary but not sufficient for p53/TP53-mediated caspase activation and apoptosis. Has a role in cell trafficking, notably of the Schwann cell, and is necessary for the maintenance and development of the peripheral nerve myelin sheath. Required for vesicular recycling of CDH1 and TF. May also function in lipid trafficking. Protects cells from spindle disruption damage. Functions in p53/TP53-dependent mitotic spindle checkpoint. Regulates microtubule dynamics and maintains euploidy.
See full target information NDRG1

대체 명칭 보기

CAP43, DRG1, RTP, NDRG1, Protein NDRG1, Differentiation-related gene 1 protein, N-myc downstream-regulated gene 1 protein, Nickel-specific induction protein Cap43, Reducing agents and tunicamycin-responsive protein, Rit42, DRG-1
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Product promise

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