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AB134610

Recombinant Human NDUFAF4 protein (His tag N-Terminus)

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Recombinant Human NDUFAF4 protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 175 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE, Mass Spec.

대체 명칭 보기

C6orf66, HRPAP20, HSPC125, My013, NDUFAF4, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 4, Hormone-regulated proliferation-associated protein of 20 kDa

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SDS-PAGE - Recombinant Human NDUFAF4 protein (His tag N-Terminus) (AB134610)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human NDUFAF4 protein (His tag N-Terminus) (AB134610)

15% SDS-PAGE analysis of ab134610 (3μg).

주요 정보

Purity

>85% SDS-PAGE

ab134610 was purified using conventional chromatography techniques.

발현 시스템

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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보완 제품

Primary Antibodies

AB191414

Anti-NDUFAF4 antibody [EPR15931]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-NDUFAF4 antibody [EPR15931] (AB191414)

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  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMGALVIRGIRNFNLENRAEREISKMKPSVAPRHPSTNSLLREQISLYPEVKGEIARKDEKLLSFLKDVYVDSKDPVSSLQVKAAETCQEPKEFRLPKDHHFDMINIKSIPKGKISIVEALTLLNNHKLFPETWTAEKIMQEYQLEQKDVNSLLKYFVTFEVEIFPPEDKKAIRSK","proteinLength":"Full Length","predictedMolecularWeight":"22.7 kDa","actualMolecularWeight":null,"aminoAcidEnd":175,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9P032","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

NDUFAF4 also known as Complex I Assembly Factor 2 plays an integral role in the assembly of NADH:ubiquinone oxidoreductase complex I within mitochondria. It has a mass of approximately 20 kilodaltons. This protein is widely expressed in various tissues with heightened levels found in organs with high energy demands such as the brain and heart. Functionally NDUFAF4 mediates the proper formation and stabilization of the mitochondrial complex I by contributing to the assembly of its subunits.
Biological function summary

NDUFAF4 participates in the mitochondrial electron transport chain as part of the complex I assembly. It acts as an assembly factor for complex I the first component of the chain which is important for initiating electron transport from NADH to the rest of the chain. This complex contains multiple subunits that require precise assembly and NDUFAF4 ensures the correct incorporation of these subunits. Its role is essential for maintaining efficient mitochondrial respiration thereby supporting ATP production.

Pathways

The NDUFAF4 protein ensures proper electron transport chain function by facilitating complex I assembly. It interacts within mitochondrial pathways involving oxidative phosphorylation and reactive oxygen species (ROS) regulation. It associates with other proteins such as NDUFS3 and NDUFS4 as part of the complex I formation process. Progressive assembly of complex I facilitated by NDUFAF4 is an important step in maintaining cellular energy metabolism.

Improper function or expression of NDUFAF4 leads to mitochondrial disorders including Leigh syndrome and other metabolic encephalopathies. Mutations in NDUFAF4 disrupt complex I assembly resulting in impaired mitochondrial respiration and energy production. This protein also relates to Parkinson’s disease where its involvement in complex I assembly connects it to proteins like Parkin which are associated with mitochondrial dysfunction in dopaminergic neurons.
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일반 정보

기능

Involved in the assembly of mitochondrial NADH : ubiquinone oxidoreductase complex (complex I) (PubMed : 18179882, PubMed : 28853723). May be involved in cell proliferation and survival of hormone-dependent tumor cells. May be a regulator of breast tumor cell invasion.

서열 유사성

Belongs to the NDUFAF4 family.

Post-translational modifications

Phosphorylated on serine. Prolactin stimulate serine phosphorylation (By similarity).

Subcellular localisation

Mitochondrion

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제품 프로토콜

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타겟 정보

Involved in the assembly of mitochondrial NADH : ubiquinone oxidoreductase complex (complex I) (PubMed : 18179882, PubMed : 28853723). May be involved in cell proliferation and survival of hormone-dependent tumor cells. May be a regulator of breast tumor cell invasion.
See full target information NDUFAF4
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Product promise

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