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AB95927

Recombinant human NEDD4 protein (DDDDK tag N-Terminus)

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Recombinant human NEDD4 protein (DDDDK tag N-Terminus) is a Human Fragment protein, in the 150 to 900 aa range, expressed in Baculovirus infected insect cells, with >60%, suitable for SDS-PAGE, FuncS.
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Functional Studies - Recombinant human NEDD4 protein (DDDDK tag N-Terminus) (AB95927)
  • FuncS

Unknown

Functional Studies - Recombinant human NEDD4 protein (DDDDK tag N-Terminus) (AB95927)

Image showing specific activity of ab95927.

SDS-PAGE - Recombinant human NEDD4 protein (DDDDK tag N-Terminus) (AB95927)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human NEDD4 protein (DDDDK tag N-Terminus) (AB95927)

10% SDS-PAGE analysis
Lane 1 : ab95927 (4μg)
Lane 2 : Molecular weight markers

주요 정보

Purity

>60% SDS-PAGE

Affinity purified.

발현 시스템

Baculovirus infected insect cells

Tags

DDDDK tag N-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Specific Activity: 0.0027 pmol/min/μg. Assay conditions: 50 μl reaction mix (4mM ATP, 5mM MgCl2, 1mM DTT, E1 (0.5 μg), E2 (2.8 μg), Biotin-Ubiquitin (0.5 μg) and NEDD4 (0-2 μg) in 50mM Tris pH 7.4) incubate for 30min at 37°C, then add to streptavidin coated plates and incubate for 30min at 37°C. Add antibody against NEDD4, incubate 1h. Finally, incubate 1h with the Europeum-labeled secondary antibody. Time resolved fluorescence measured at room temperature (Lambda exc=330 nm, Lambda em=620 nm). Specific Activity: 0.0027 pmol/min/μg. Assay conditions: 50 μl reaction mix (4mM ATP, 5mM MgCl2, 1mM DTT, E1 (0.5 μg), E2 (2.8 μg), Biotin-Ubiquitin (0.5 μg) and NEDD4 (0-2 μg) in 50mM Tris pH 7.4) incubate for 30min at 37°C, then add to streptavidin coated plates and incubate for 30min at 37°C. Add antibody against NEDD4, incubate 1h. Finally, incubate 1h with the Europeum-labeled secondary antibody. Time resolved fluorescence measured at room temperature (Lambda exc=330 nm, Lambda em=620 nm).

Accession

P46934-1

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.395% Tris HCl, 0.05% Sorbitan monolaurate, ethoxylated, 0.0462% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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보완 제품

Reagents, Controls & Accessories

AB234592

Prestained Protein Ladder - Extra broad molecular weight (6.5 - 270 kDa)

SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (6.5 - 270 kDa) (AB234592)

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AB95930

Recombinant human SMURF 2 protein (DDDDK tag N-Terminus)

Functional Studies - Recombinant human SMURF 2 protein (DDDDK tag N-Terminus) (AB95930)

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Proteins & Peptides

AB268946

Recombinant human RNF34 protein (Active) (GST tag N-Terminus)

Functional Studies - Recombinant human RNF34 protein (Active) (AB268946)

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Proteins & Peptides

AB317878

Recombinant Human UBE2D3 protein (His-tag)

Mass Spectrometry - Recombinant Human UBE2D3 protein (His-tag) (AB317878)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"","proteinLength":"Fragment","predictedMolecularWeight":"86 kDa","actualMolecularWeight":null,"aminoAcidEnd":900,"aminoAcidStart":150,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P46934","tags":[{"tag":"DDDDK","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification
배송 시 보관 조건
Dry Ice
적절한 단기 보관 조건
-80°C
적절한 장기 보관 조건
-80°C
True
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

NEDD4 protein also called NEDD4-1 is an E3 ubiquitin-protein ligase. It has a role in tagging proteins with ubiquitin molecules marking them for degradation by the proteasome. This protein has a molecular mass of approximately 120 kDa. High expression of NEDD4 occurs in tissues like the brain heart and skeletal muscle. The activity of NEDD4 impacts various processes ensuring protein quality control and regulation within cells.
Biological function summary

The impact of NEDD4 protein extends beyond its basic mechanical function. It plays an important role in cell signaling development and even adjusting cell volume. NEDD4 participates in forming a ubiquitin-protein ligase complex engaging with multiple substrates to transfer ubiquitin. It modulates key signaling pathways influencing cellular responses and maintaining healthy cell functioning.

Pathways

NEDD4 activity is embedded in key cellular processes. It is especially integral in the PI3K/AKT signaling and Wnt signaling pathways where it can influence signal transduction and cellular growth. NEDD4 ubiquitin ligase family members like NEDD4-2 are often seen working alongside it. These pathways involve other proteins such as PTEN which NEDD4 regulates affecting cell proliferation and survival.

NEDD4 holds significant connections to cancer and neurodegenerative diseases. Elevated levels of NEDD4 can contribute to oncogenesis particularly in cancers where dysregulation in protein degradation occurs. The aberrant activity of NEDD4 links to neurodegenerative diseases as it affects the turnover of neuronal proteins. It shares a complex relationship with PTEN in diseases considering their interaction can alter cellular pathways often distorted in pathological conditions.
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일반 정보

기능

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Specifically ubiquitinates 'Lys-63' in target proteins (PubMed : 19920177, PubMed : 21399620, PubMed : 23644597). Involved in the pathway leading to the degradation of VEGFR-2/KDFR, independently of its ubiquitin-ligase activity. Monoubiquitinates IGF1R at multiple sites, thus leading to receptor internalization and degradation in lysosomes (By similarity). Ubiquitinates FGFR1, leading to receptor internalization and degradation in lysosomes (PubMed : 21765395). Promotes ubiquitination of RAPGEF2 (PubMed : 11598133). According to PubMed : 18562292 the direct link between NEDD4 and PTEN regulation through polyubiquitination described in PubMed : 17218260 is questionable. Involved in ubiquitination of ERBB4 intracellular domain E4ICD (By similarity). Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development (By similarity). Ubiquitinates TNK2 and regulates EGF-induced degradation of EGFR and TNF2 (PubMed : 20086093). Ubiquitinates BRAT1 and this ubiquitination is enhanced in the presence of NDFIP1 (PubMed : 25631046). Ubiquitinates DAZAP2, leading to its proteasomal degradation (PubMed : 11342538). Ubiquitinates POLR2A (PubMed : 19920177). Functions as a platform to recruit USP13 to form an NEDD4-USP13 deubiquitination complex that plays a critical role in cleaving the 'Lys-48'-linked ubiquitin chains of VPS34 and then stabilizing VPS34, thus promoting the formation of autophagosomes (PubMed : 32101753).. (Microbial infection) Involved in the ubiquitination of Ebola virus protein VP40 which plays a role in viral budding.

Post-translational modifications

Undergoes 'Lys-29'-linked auto-ubiquitination at Lys-1279 and serves as a scaffold for recruiting USP13 to form an NEDD4-USP13 deubiquitination complex.

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제품 프로토콜

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타겟 정보

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Specifically ubiquitinates 'Lys-63' in target proteins (PubMed : 19920177, PubMed : 21399620, PubMed : 23644597). Involved in the pathway leading to the degradation of VEGFR-2/KDFR, independently of its ubiquitin-ligase activity. Monoubiquitinates IGF1R at multiple sites, thus leading to receptor internalization and degradation in lysosomes (By similarity). Ubiquitinates FGFR1, leading to receptor internalization and degradation in lysosomes (PubMed : 21765395). Promotes ubiquitination of RAPGEF2 (PubMed : 11598133). According to PubMed : 18562292 the direct link between NEDD4 and PTEN regulation through polyubiquitination described in PubMed : 17218260 is questionable. Involved in ubiquitination of ERBB4 intracellular domain E4ICD (By similarity). Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development (By similarity). Ubiquitinates TNK2 and regulates EGF-induced degradation of EGFR and TNF2 (PubMed : 20086093). Ubiquitinates BRAT1 and this ubiquitination is enhanced in the presence of NDFIP1 (PubMed : 25631046). Ubiquitinates DAZAP2, leading to its proteasomal degradation (PubMed : 11342538). Ubiquitinates POLR2A (PubMed : 19920177). Functions as a platform to recruit USP13 to form an NEDD4-USP13 deubiquitination complex that plays a critical role in cleaving the 'Lys-48'-linked ubiquitin chains of VPS34 and then stabilizing VPS34, thus promoting the formation of autophagosomes (PubMed : 32101753).. (Microbial infection) Involved in the ubiquitination of Ebola virus protein VP40 which plays a role in viral budding.
See full target information NEDD4

대체 명칭 보기

KIAA0093, NEDD4-1, RPF1, PIG53, NEDD4, E3 ubiquitin-protein ligase NEDD4, Cell proliferation-inducing gene 53 protein, HECT-type E3 ubiquitin transferase NEDD4, Neural precursor cell expressed developmentally down-regulated protein 4, NEDD-4
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Product promise

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