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AB60703

Recombinant human NEK7 protein (GST tag N-Terminus)

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(1 출판물)

Recombinant human NEK7 protein (GST tag N-Terminus) is a Human Full Length protein, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, FuncS.
5 이미지
Functional Studies - Recombinant human NEK7 protein (AB60703)
  • FuncS

Unknown

Functional Studies - Recombinant human NEK7 protein (AB60703)

The specific activity of NEK7 (ab60703) was determined to be 9.2 nmol/min/mg as per activity assay protocol and was equivalent to 188 nmol/min/mg as per radiometric assay

Functional Studies - Recombinant human NEK7 protein (AB60703)
  • FuncS

Unknown

Functional Studies - Recombinant human NEK7 protein (AB60703)

Sample Kinase Activity Plot.

Functional Studies - Recombinant human NEK7 protein (AB60703)
  • FuncS

Unknown

Functional Studies - Recombinant human NEK7 protein (AB60703)

The specific activity of NEK7 (ab60703) was determined to be 9.2 nmol/min/mg as per activity assay protocol and was equivalent to 188 nmol/min/mg as per radiometric assay

SDS-PAGE - Recombinant human NEK7 protein (AB60703)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human NEK7 protein (AB60703)

ab60703 on SDS-PAGE, MW ~63kDa.

SDS-PAGE - Recombinant human NEK7 protein (AB60703)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human NEK7 protein (AB60703)

SDS PAGE analysis of ab60703

주요 정보

Purity

>90% SDS-PAGE

Purity: >90% as determined by densitometry. Affinity purified.

발현 시스템

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

Active

Accession

Q8TDX7-1

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.0038% EGTA, 0.00292% EDTA, 0.00174% PMSF

storage-buffer

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보완 제품

Reagents, Controls & Accessories

AB313996

Prestained Protein Ladder - Extra broad molecular weight (3 - 260 kDa)

SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (3 - 260 kDa) (AB313996)

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  • 0
Proteins & Peptides

AB320811

Recombinant Mouse NLRP3 Protein Standard (His tag)

Sandwich ELISA - Recombinant Mouse NLRP3 Protein Standard (His tag) (AB320811)

  • 0
  • 0
Proteins & Peptides

AB107953

Recombinant human MAP4K1/HPK1 protein (GST tag N-Terminus)

Functional Studies - Recombinant human MAP4K1/HPK1 protein (AB107953)

  • 0
  • 0
Proteins & Peptides

AB56641

Recombinant human PKC theta/PRKCQ protein (GST tag N-Terminus)

Western blot - Recombinant human PKC theta/PRKCQ protein (GST tag N-Terminus) (AB56641)

  • 0
  • 0
Primary Antibodies

AB133514

Anti-NEK7 antibody [EPR4900]

20ul selling size|RabMAb|Recombinant|KO Validated|Trial Size

Immunoprecipitation - Anti-NEK7 antibody [EPR4900] (AB133514)

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  • 2
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Kinase assay</p>" } } }
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제품 세부 정보

ab91092 (Cow Casein full length protein) can be utilized as a substrate for assessing Kinase activity
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서열 정보

[{"linker":null,"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q8TDX7","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification
배송 시 보관 조건
Dry Ice
적절한 단기 보관 조건
-80°C
적절한 장기 보관 조건
-80°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
True
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The NIMA-related kinase 7 (NEK7) is a member of the NEK family of serine/threonine kinases and plays an important role in cell division. With a molecular mass of approximately 34 kDa the NEK7 protein is involved in mitosis by regulating microtubule organization. It is primarily expressed in various tissues including human and marmoset thyroid tissues. It remains mainly inactive until mitotic entry where its activity becomes essential for proper spindle formation and completion of mitosis.
Biological function summary

Cell cycle progression heavily depends on NEK7's function. NEK7 interacts with NEDD1 in the microtubule-organizing center contributing to centrosome maturation and separation. This interaction makes NEK7 vital in the recruitment of γ-tubulin ring complex which is necessary for nucleating microtubules. NEK7 operates independently from the other NEK family members but shares involvement in centrosome-related activities.

Pathways

NEK7 is influential in the regulation of the mitotic spindle assembly pathway and the interleukin-1 (IL-1) signaling pathway. It is closely related to proteins like NEK6 and NEK9 which also play roles in mitotic progression and cytokinesis. NEK7's involvement in these pathways indicates its integrative function in ensuring normal cell division and response to inflammatory signals.

NEK7 has implications in cancer and inflammatory ailments. NEK7 overexpression or mutation is often linked to tumorigenesis particularly in lung and brain cancers. Additionally NEK7 contributes to the activation of the NLRP3 inflammasome connecting it to inflammatory conditions like rheumatoid arthritis. The interplay with NLRP3 highlights the significance of NEK7 in inflammation-related diseases.
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일반 정보

기능

Protein kinase which plays an important role in mitotic cell cycle progression (PubMed : 17101132, PubMed : 19941817, PubMed : 31409757). Required for microtubule nucleation activity of the centrosome, robust mitotic spindle formation and cytokinesis (PubMed : 17586473, PubMed : 19414596, PubMed : 19941817, PubMed : 26522158, PubMed : 31409757). Phosphorylates EML4 at 'Ser-146', promoting its dissociation from microtubules during mitosis which is required for efficient chromosome congression (PubMed : 31409757). Phosphorylates RPS6KB1 (By similarity). Acts as an essential activator of the NLRP3 inflammasome assembly independently of its kinase activity (PubMed : 26642356, PubMed : 36442502, PubMed : 39173637). Acts by unlocking NLRP3 following NLRP3 tranlocation into the microtubule organizing center (MTOC), relieving NLRP3 autoinhibition and promoting formation of the NLRP3 : PYCARD complex, and activation of CASP1 (PubMed : 26642356, PubMed : 31189953, PubMed : 36442502, PubMed : 39173637, PubMed : 40450990). Serves as a cellular switch that enforces mutual exclusivity of the inflammasome response and cell division : interaction with NEK9 prevents interaction with NLRP3 and activation of the inflammasome during mitosis (PubMed : 26642356, PubMed : 31189953).

서열 유사성

Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.

Post-translational modifications

Phosphorylation at Ser-195 required for its activation.

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제품 프로토콜

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타겟 정보

Protein kinase which plays an important role in mitotic cell cycle progression (PubMed : 17101132, PubMed : 19941817, PubMed : 31409757). Required for microtubule nucleation activity of the centrosome, robust mitotic spindle formation and cytokinesis (PubMed : 17586473, PubMed : 19414596, PubMed : 19941817, PubMed : 26522158, PubMed : 31409757). Phosphorylates EML4 at 'Ser-146', promoting its dissociation from microtubules during mitosis which is required for efficient chromosome congression (PubMed : 31409757). Phosphorylates RPS6KB1 (By similarity). Acts as an essential activator of the NLRP3 inflammasome assembly independently of its kinase activity (PubMed : 26642356, PubMed : 36442502, PubMed : 39173637). Acts by unlocking NLRP3 following NLRP3 tranlocation into the microtubule organizing center (MTOC), relieving NLRP3 autoinhibition and promoting formation of the NLRP3 : PYCARD complex, and activation of CASP1 (PubMed : 26642356, PubMed : 31189953, PubMed : 36442502, PubMed : 39173637, PubMed : 40450990). Serves as a cellular switch that enforces mutual exclusivity of the inflammasome response and cell division : interaction with NEK9 prevents interaction with NLRP3 and activation of the inflammasome during mitosis (PubMed : 26642356, PubMed : 31189953).
See full target information NEK7

대체 명칭 보기

Serine/threonine-protein kinase Nek7, Never in mitosis A-related kinase 7, NimA-related protein kinase 7, NEK7
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

EMBO reports 23:e53499 PubMed34882936

2021

Licochalcone B specifically inhibits the NLRP3 inflammasome by disrupting NEK7-NLRP3 interaction.

Applications

Unspecified application

Species

Unspecified reactive species

Qiang Li,Hui Feng,Hongbo Wang,Yinghao Wang,Wenqing Mou,Guang Xu,Ping Zhang,Ruisheng Li,Wei Shi,Zhilei Wang,Zhie Fang,Lutong Ren,Yan Wang,Li Lin,Xiaorong Hou,Wenzhang Dai,Zhiyong Li,Ziying Wei,Tingting Liu,Jiabo Wang,Yuming Guo,Pengyan Li,Xu Zhao,Xiaoyan Zhan,Xiaohe Xiao,Zhaofang Bai
제품이 사용된 논문 모두 보기
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Product promise

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