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AB221023

Recombinant human Neuropilin 1 protein (Fc Chimera Active)

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Recombinant human Neuropilin 1 protein (Fc Chimera Active) is a Human Full Length protein, in the 22 to 644 aa range, expressed in HEK 293 cells, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS.

대체 명칭 보기

CD304, NRP, VEGF165R, NRP1, Neuropilin-1, Vascular endothelial cell growth factor 165 receptor

3 Images
Functional Studies - Recombinant human Neuropilin 1 protein (Fc Chimera Active) (AB221023)
  • FuncS

Supplier Data

Functional Studies - Recombinant human Neuropilin 1 protein (Fc Chimera Active) (AB221023)

Immobilized ab221023 at 2 μg/mL (100 μL/well) can bind Biotinylated Human VEGF165 with a linear range of 5-40 ng/mL.

Functional Studies - Recombinant human Neuropilin 1 protein (Fc Chimera Active) (AB221023)
  • FuncS

Supplier Data

Functional Studies - Recombinant human Neuropilin 1 protein (Fc Chimera Active) (AB221023)

Immobilized Human VEGF165 (Tag Free) at 2 µg/mL (100 µL/well) can bind Human NRP1 (Fc Tag) with a linear range of 0.2-3 ng/mL (QC tested).

SDS-PAGE - Recombinant human Neuropilin 1 protein (Fc Chimera Active) (AB221023)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human Neuropilin 1 protein (Fc Chimera Active) (AB221023)

SDS-PAGE analysis of ab221023 under reducing conditions, stained overnight with Coomassie Blue. As a result of glycosylation, the reduced protein migrates as 120-125 kDa.

주요 정보

Purity

>95% SDS-PAGE

Endotoxin 레벨

< 1 EU/µg

발현 시스템

HEK 293 cells

Tags

Fc tag C-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Measured by its binding ability in a functional ELISA. Immobilized ab221023 at 2 μg/mL (100 μL/well) can bind Biotinylated Human VEGF165 with a linear range of 5-40 ng/mL.

Accession

O14786

Animal free

No

Carrier free

No

Species

Human

Reconstitution

Reconstitute at 200 µg/mL in water

보관 버퍼

pH: 7.4 Constituents: L-Arginine, Sodium chloride, 5% Trehalose, 0.75% Glycine, 0.61% Tris

storage-buffer

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보완 제품

Reagents, Controls & Accessories

AB313996

Prestained Protein Ladder - Extra broad molecular weight (3 - 260 kDa)

SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (3 - 260 kDa) (AB313996)

  • 0
  • 0
Primary Antibodies

AB81321

Anti-Neuropilin 1 antibody [EPR3113]

RabMAb|Recombinant|KO Validated|20ul selling size

Flow Cytometry (Intracellular) - Anti-Neuropilin 1 antibody [EPR3113] (AB81321)

  • 4
  • 31
Primary Antibodies

AB52894

Anti-EGFR antibody [EP38Y]

BOND RX™ Validated|KO Validated|RabMAb|Lab Essentials|20ul selling size|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [EP38Y] (AB52894)

  • 5
  • 24
Assay Kits

AB227901

Human Neuropilin-1 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Neuropilin-1 ELISA Kit (AB227901)

  • 0
  • 0
Proteins & Peptides

AB189308

Neuropilin 1 peptide

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"sequence":"FRNDKCGDTIKIESPGYLTSPGYPHSYHPSEKCEWLIQAPDPYQRIMINFNPHFDLEDRDCKYDYVEVFDGENENGHFRGKFCGKIAPPPVVSSGPFLFIKFVSDYETHGAGFSIRYEIFKRGPECSQNYTTPSGVIKSPGFPEKYPNSLECTYIVFVPKMSEIILEFESFDLEPDSNPPGGMFCRYDRLEIWDGFPDVGPHIGRYCGQKTPGRIRSSSGILSMVFYTDSAIAKEGFSANYSVLQSSVSEDFKCMEALGMESGEIHSDQITASSQYSTNWSAERSRLNYPENGWTPGEDSYREWIQVDLGLLRFVTAVGTQGAISKETKKKYYVKTYKIDVSSNGEDWITIKEGNKPVLFQGNTNPTDVVVAVFPKPLITRFVRIKPATWETGISMRFEVYGCKITDYPCSGMLGMVSGLISDSQITSSNQGDRNWMPENIRLVTSRSGWALPPAPHSYINEWLQIDLGEEKIVRGIIIQGGKHRENKVFMRKFKIGYSNNGSDWKMIMDDSKRKAKSFEGNNNYDTPELRTFPALSTRFIRIYPERATHGGLGLRMELLGCEVEAPTAGPTTPNGNLVDECDDDQANCHSGTGDDFQLTGGTTVLATEKPTVIDSTIQSGIK","proteinLength":"Full Length","predictedMolecularWeight":"96.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":644,"aminoAcidStart":22,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"O14786","tags":[{"tag":"Fc","terminus":"C-Terminus"}]}]
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특성 및 보관 정보

제형
Lyophilized
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
보관 정보
Avoid freeze / thaw cycle
True
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Neuropilin-1 also known as NRP1 is a transmembrane protein with a significant role in the nervous and vascular systems. It has a molecular mass of approximately 130 kDa. Neuropilin-1 expression occurs broadly in tissues including neurons endothelial cells and tumor cells. Its structure includes a large extracellular domain that binds various ligands mediating several cellular functions. Neuropilin-1 is sometimes targeted in experiments using methods like neuropilin-1 ELISA and PE immunofluorescence to study its distribution and function in different tissues.
Biological function summary

Neuropilin-1 serves as a co-receptor for both the Vascular Endothelial Growth Factor (VEGF) and Semaphorin family proteins. It plays an important role in processes such as angiogenesis axonal guidance and the immune system. Neuropilin-1 does not function alone; it forms complexes with neuropilin-2 and other receptors like Plexin and VEGFR enhancing signal transduction pathways for angiogenesis and neuronal development. This involvement allows cells to respond appropriately to their environment especially during organismal development and repair processes.

Pathways

Neuropilin-1 facilitates interactions within the VEGF and Semaphorin pathways. In the VEGF pathway Neuropilin-1 enhances binding and signaling efficiency with VEGF closely working alongside VEGFR to promote endothelial cell survival migration and new blood vessel formation. In the Semaphorin pathway Neuropilin-1 interacts with Plexins mediating neuronal pathfinding and axonal growth. These interactions highlight Neuropilin-1's adaptive capabilities in various physiological processes critical for system development.

Neuropilin-1 is linked to pathological conditions like cancer and cardiovascular diseases. Neuropilin-1 overexpression is frequently observed in tumors driving cancer progression through enhanced angiogenesis and tissue invasion closely interacting with proteins like VEGF-A. In cardiovascular disease Neuropilin-1 contributes to abnormal blood vessel formation and stability. By studying Neuropilin-1 and other biomarkers like CD304 FITC researchers aim to develop therapeutic strategies targeting its role in these diseases.
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일반 정보

기능

Cell-surface receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. Mediates the chemorepulsant activity of semaphorins (PubMed : 10688880, PubMed : 9288753, PubMed : 9529250). Recognizes a C-end rule (CendR) motif R/KXXR/K on its ligands which causes cellular internalization and vascular leakage (PubMed : 19805273). It binds to semaphorin 3A, the PLGF-2 isoform of PGF, the VEGF165 isoform of VEGFA and VEGFB (PubMed : 10688880, PubMed : 19805273, PubMed : 9288753, PubMed : 9529250). Coexpression with KDR results in increased VEGF165 binding to KDR as well as increased chemotaxis. Regulates VEGF-induced angiogenesis. Binding to VEGFA initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity). Regulates mitochondrial iron transport via interaction with ABCB8/MITOSUR (PubMed : 30623799).. (Microbial infection) Acts as a host factor for human coronavirus SARS-CoV-2 infection. Recognizes and binds to CendR motif RRAR on SARS-CoV-2 spike protein S1 which enhances SARS-CoV-2 infection.. Isoform 2. Binds VEGF-165 and may inhibit its binding to cells (PubMed : 10748121, PubMed : 26503042). May induce apoptosis by sequestering VEGF-165 (PubMed : 10748121). May bind as well various members of the semaphorin family. Its expression has an averse effect on blood vessel number and integrity.

서열 유사성

Belongs to the neuropilin family.

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제품 프로토콜

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타겟 정보

Cell-surface receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. Mediates the chemorepulsant activity of semaphorins (PubMed : 10688880, PubMed : 9288753, PubMed : 9529250). Recognizes a C-end rule (CendR) motif R/KXXR/K on its ligands which causes cellular internalization and vascular leakage (PubMed : 19805273). It binds to semaphorin 3A, the PLGF-2 isoform of PGF, the VEGF165 isoform of VEGFA and VEGFB (PubMed : 10688880, PubMed : 19805273, PubMed : 9288753, PubMed : 9529250). Coexpression with KDR results in increased VEGF165 binding to KDR as well as increased chemotaxis. Regulates VEGF-induced angiogenesis. Binding to VEGFA initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity). Regulates mitochondrial iron transport via interaction with ABCB8/MITOSUR (PubMed : 30623799).. (Microbial infection) Acts as a host factor for human coronavirus SARS-CoV-2 infection. Recognizes and binds to CendR motif RRAR on SARS-CoV-2 spike protein S1 which enhances SARS-CoV-2 infection.. Isoform 2. Binds VEGF-165 and may inhibit its binding to cells (PubMed : 10748121, PubMed : 26503042). May induce apoptosis by sequestering VEGF-165 (PubMed : 10748121). May bind as well various members of the semaphorin family. Its expression has an averse effect on blood vessel number and integrity.
See full target information NRP1
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Product promise

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