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AB112396

Recombinant Human NFAT5 protein (GST tag N-Terminus)

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Recombinant Human NFAT5 protein (GST tag N-Terminus) is a Human Fragment protein, in the 1422 to 1531 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

대체 명칭 보기

KIAA0827, TONEBP, NFAT5, Nuclear factor of activated T-cells 5, NF-AT5, T-cell transcription factor NFAT5, Tonicity-responsive enhancer-binding protein, TonE-binding protein, TonEBP

1 이미지
SDS-PAGE - Recombinant Human NFAT5 protein (GST tag N-Terminus) (AB112396)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human NFAT5 protein (GST tag N-Terminus) (AB112396)

12.5% SDS-PAGE analysis of NFAT5 protein (ab112396), stained with Coomassie Blue.

주요 정보

발현 시스템

Wheat germ

Tags

GST tag N-Terminus

Applications

SDS-PAGE, ELISA, WB

applications

Biologically active

No

Accession

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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보완 제품

Primary Antibodies

AB3446

Anti-NFAT5 antibody

Immunoprecipitation - Anti-NFAT5 antibody (AB3446)

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  • 1
Proteins & Peptides

AB4978

NFAT5 peptide

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  • 1
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>(recombinant protein).</p>" } } }
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서열 정보

[{"linker":null,"sequence":"FHNTAGGTMNQLQNSPGSSQQTSGMFLFGIQNNCSQLLTSGPATLPDQLMAISQPGQPQNEGQPPVTTLLSQQMPENSPLASSINTNQNIEKIDLLVSLQNQGNNLTGSF","proteinLength":"Fragment","predictedMolecularWeight":"37.73 kDa","actualMolecularWeight":null,"aminoAcidEnd":1531,"aminoAcidStart":1422,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"O94916","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Proprietary technique
배송 시 보관 조건
Dry Ice
적절한 단기 보관 조건
-80°C
적절한 장기 보관 조건
-80°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

NFAT5 also known as TonEBP (tonicity-responsive enhancer binding protein) is a transcription factor belonging to the nuclear factor of activated T-cells (NFAT) family. It is involved in mechanistic functions through its role in the osmoregulatory mechanism aiding cells to adapt to changes in osmotic stress by regulating gene expression related to maintaining ionic and osmotic balance. NFAT5 has a molecular mass of approximately 170 kDa and is primarily expressed in kidney medulla cells where osmotic stress is prevalent.
Biological function summary

NFAT5 helps regulate the cellular response to hypertonic stress in several tissues including the kidneys brain and immune cells. It acts as a transcriptional activator that induces the expression of osmoprotective genes such as the sodium/myo-inositol transporter and aldose reductase. NFAT5 functions not as part of a larger complex but as an independent transcription factor which directly binds to enhancers within target gene promotors playing a critical role in cellular adaptation to hyperosmotic conditions.

Pathways

NFAT5 plays a significant role in the hypertonicity-induced signaling pathway and the osmoprotective pathway. Through these it influences key cellular processes by modulating the transcription of genes necessary for adaptation to osmotic stress. It stands in relation to other NFAT family members like NFATc1 and NFATc2 but distinctively differs as it does not rely directly on calcium and calcineurin for its activation unlike its relatives involved in the calcineurin/NFAT pathway.

NFAT5 has implications in cardiovascular diseases and diabetes mellitus. It's involved in cellular responses to hypertonic stress in various disease states notably influencing the vascular smooth muscle cell function in hypertension. Additionally NFAT5's regulation of osmoprotective genes connects it to metabolic disorders like diabetes where osmotic stress responses are disturbed. Interactions between NFAT5 and other proteins like TFEB (Transcription Factor EB) occur during conditions related to metabolic disorders indicating its broad relevance in stress-related pathologies.
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일반 정보

기능

Transcription factor involved, among others, in the transcriptional regulation of osmoprotective and inflammatory genes. Binds the DNA consensus sequence 5'-[ACT][AG]TGGAAA[CAT]A[TA][ATC][CA][ATG][GT][GAC][CG][CT]-3' (PubMed : 10377394). Mediates the transcriptional response to hypertonicity (PubMed : 10051678). Positively regulates the transcription of LCN2 and S100A4 genes; optimal transactivation of these genes requires the presence of DDX5/DDX17 (PubMed : 22266867). Also involved in the DNA damage response by preventing formation of R-loops; R-loops are composed of a DNA : RNA hybrid and the associated non-template single-stranded DNA (PubMed : 34049076).

Post-translational modifications

Phosphorylated (PubMed:10377394). Phosphorylated at Thr-135 by CDK5 in response to osmotic stress; this phosphorylation mediates its rapid nuclear localization (PubMed:21209322).. Poly-ADP-ribosylated by PARP1 in response to DNA damage, promoting recruitment to sites of R-loop-associated DNA damage.

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제품 프로토콜

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타겟 정보

Transcription factor involved, among others, in the transcriptional regulation of osmoprotective and inflammatory genes. Binds the DNA consensus sequence 5'-[ACT][AG]TGGAAA[CAT]A[TA][ATC][CA][ATG][GT][GAC][CG][CT]-3' (PubMed : 10377394). Mediates the transcriptional response to hypertonicity (PubMed : 10051678). Positively regulates the transcription of LCN2 and S100A4 genes; optimal transactivation of these genes requires the presence of DDX5/DDX17 (PubMed : 22266867). Also involved in the DNA damage response by preventing formation of R-loops; R-loops are composed of a DNA : RNA hybrid and the associated non-template single-stranded DNA (PubMed : 34049076).
See full target information NFAT5
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Product promise

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