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AB61262

Recombinant Human NFkB Inducing Kinase NIK protein (GST tag N-Terminus)

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Recombinant Human NFkB Inducing Kinase NIK protein (GST tag N-Terminus) is a Human Fragment protein, in the 318 to 947 aa range, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, FuncS.
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SDS-PAGE - Recombinant Human NFkB Inducing Kinase NIK protein (GST tag N-Terminus) (AB61262)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human NFkB Inducing Kinase NIK protein (GST tag N-Terminus) (AB61262)

10% SDS-PAGE, 4 μg ab61262, coomassie stained.

주요 정보

Purity

>90% SDS-PAGE

Affinity purified.

발현 시스템

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

SDS-PAGE, FuncS

applications

Biologically active

No

Accession

Q99558-1

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 8 Constituents: 50% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.395% Tris HCl, 0.05% Sorbitan monolaurate, ethoxylated, 0.0462% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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보완 제품

Reagents, Controls & Accessories

AB116029

Prestained Protein Ladder - Extra broad molecular weight (5 - 245 kDa)

SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (5 - 245 kDa) (AB116029)

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  • 1
Proteins & Peptides

AB101775

Recombinant human MAP4K4/NIK protein (GST tag N-Terminus)

Functional Studies - Recombinant human MAP4K4/NIK protein (GST tag N-Terminus) (AB101775)

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  • 0
Proteins & Peptides

AB114410

Recombinant Human cIAP1 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human cIAP1 protein (GST tag N-Terminus) (AB114410)

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Proteins & Peptides

AB271732

Recombinant human RIP protein (Active) (GST tag N-Terminus)

Functional Studies - Recombinant human RIP protein (Active) (GST tag N-Terminus) (AB271732)

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Proteins & Peptides

AB52101

Recombinant human Cleaved Caspase-3 protein (Active)

Western blot - Recombinant human Cleaved Caspase-3 protein (Active) (AB52101)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Kinase Assay: Use at an assay dependent dilution. 20 nM NIK yields 20% conversion of 1 uM ATP using MBP as substrate.</p>" } } }
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서열 정보

[{"linker":null,"sequence":"","proteinLength":"Fragment","predictedMolecularWeight":"104 kDa","actualMolecularWeight":null,"aminoAcidEnd":947,"aminoAcidStart":318,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q99558","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification
배송 시 보관 조건
Dry Ice
적절한 단기 보관 조건
-80°C
적절한 장기 보관 조건
-80°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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일반 정보

기능

Lymphotoxin beta-activated kinase which seems to be exclusively involved in the activation of NF-kappa-B and its transcriptional activity. Phosphorylates CHUK/IKKA, thereby promoting proteolytic processing of NFKB2/P100, which leads to NF-kappa-B activation via the non-canonical pathway (PubMed : 25406581, PubMed : 29230214). Has an essential role in the non-canonical NF-kappa-B signaling that regulates genes encoding molecules involved in B-cell survival, lymphoid organogenesis, and immune response (PubMed : 25406581). Could act in a receptor-selective manner.

서열 유사성

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.

Post-translational modifications

Autophosphorylated. Phosphorylation at Thr-559 is required to activate its kinase activity and 'Lys-63'-linked polyubiquitination. Phosphorylated by CHUK/IKKA leading to MAP3K14 destabilization.. Ubiquitinated. Undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. 'Lys-48'-linked polyubiquitination leads to its degradation by the proteasome, while 'Lys-63'-linked polyubiquitination stabilizes and activates it.

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제품 프로토콜

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타겟 정보

Lymphotoxin beta-activated kinase which seems to be exclusively involved in the activation of NF-kappa-B and its transcriptional activity. Phosphorylates CHUK/IKKA, thereby promoting proteolytic processing of NFKB2/P100, which leads to NF-kappa-B activation via the non-canonical pathway (PubMed : 25406581, PubMed : 29230214). Has an essential role in the non-canonical NF-kappa-B signaling that regulates genes encoding molecules involved in B-cell survival, lymphoid organogenesis, and immune response (PubMed : 25406581). Could act in a receptor-selective manner.
See full target information MAP3K14

대체 명칭 보기

NIK, MAP3K14, Mitogen-activated protein kinase kinase kinase 14, NF-kappa-beta-inducing kinase, Serine/threonine-protein kinase NIK, HsNIK
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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