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AB107700

Recombinant human NLK protein (GST tag N-Terminus)

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Recombinant human NLK protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 527 aa range, expressed in Baculovirus infected Sf9 cells, with >85%, suitable for SDS-PAGE, WB, FuncS.
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Functional Studies - Recombinant human NLK protein (GST tag N-Terminus) (AB107700)
  • FuncS

Unknown

Functional Studies - Recombinant human NLK protein (GST tag N-Terminus) (AB107700)

Specific activity of ab107700 was determined to be 2.4 nmol/min/mg.

SDS-PAGE - Recombinant human NLK protein (GST tag N-Terminus) (AB107700)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human NLK protein (GST tag N-Terminus) (AB107700)

SDS-PAGE analysis of ab107700

주요 정보

Purity

>85% SDS-PAGE

The purity was determined to be >85% by densitometry.GST Affinity Column purified.

발현 시스템

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

FuncS, SDS-PAGE, WB

applications

Biologically active

Yes

Biological activity

Specific activity: 2.4 nmol/min/mg

Accession

Q9UBE8-1

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.307% Glutathione, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00292% EDTA, 0.00174% PMSF

storage-buffer

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보완 제품

Reagents, Controls & Accessories

AB234592

Prestained Protein Ladder - Extra broad molecular weight (6.5 - 270 kDa)

SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (6.5 - 270 kDa) (AB234592)

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  • 0
Primary Antibodies

AB97642

Anti-NLK antibody

Immunocytochemistry/ Immunofluorescence - Anti-NLK antibody (AB97642)

  • 4
  • 4
Primary Antibodies

AB247773

Anti-Plastin L antibody [EPR4278] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4278] - BSA and Azide free (AB247773)

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Assay Kits

AB139418

Propidium Iodide Flow Cytometry Kit

Flow Cytometry - Propidium Iodide Flow Cytometry Kit (AB139418)

  • 0
  • 0
Assay Kits

AB287852

Cell Cycle Analysis Kit

Functional Studies - Cell Cycle Analysis Kit (AB287852)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity
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서열 정보

[{"linker":null,"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":"84 kDa","actualMolecularWeight":null,"aminoAcidEnd":527,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q9UBE8","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification GST Tag
배송 시 보관 조건
Dry Ice
적절한 단기 보관 조건
-80°C
적절한 장기 보관 조건
-80°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
True
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

NLK (Nemo-Like Kinase) functions as a serine/threonine protein kinase involved in various signaling pathways. It alternatively is known as nemo-like protein or nlk. The molecular mass of NLK is approximately 54 kDa. Expression of NLK occurs in various tissues but it is highly observed in the brain and testis. This wide expression suggests that NLK may have diverse functional roles.
Biological function summary

NLK participates in regulating cellular processes such as cell cycle apoptosis and transcription. It is not part of a larger complex but works within signaling pathways to influence these processes. NLK modulates the activity of several transcription factors particularly in the Wnt/beta-catenin signaling pathway where it negatively regulates the signaling to control gene expression relevant to cell proliferation and differentiation.

Pathways

NLK is an important player in the Wnt/beta-catenin and MAPK pathways. In the Wnt/beta-catenin pathway it associates with proteins such as TCF/LEF and beta-catenin acting to modulate transcription by phosphorylating these molecules. The MAPK pathway involves NLK in response to stress signals coordinating with proteins like TAK1 to mediate cellular responses. These pathways illustrate NLK's role in transmitting signals that govern cell fate and stress responses.

NLK has links to cancer and neurodegenerative diseases. Alterations in NLK expression or function can contribute to the development and progression of cancers by affecting the regulation of cell proliferation and apoptosis. Specifically dysregulation in the Wnt/beta-catenin pathway in which NLK acts relates to colorectal cancers. In the context of neurodegenerative diseases NLK has connections with the protein TDP-43 which is implicated in diseases such as amyotrophic lateral sclerosis highlighting its role in neuronal health and potential links to neuronal degradation.
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일반 정보

기능

Serine/threonine-protein kinase that regulates a number of transcription factors with key roles in cell fate determination (PubMed : 12482967, PubMed : 14960582, PubMed : 15004007, PubMed : 15764709, PubMed : 20061393, PubMed : 20874444, PubMed : 21454679). Positive effector of the non-canonical Wnt signaling pathway, acting downstream of WNT5A, MAP3K7/TAK1 and HIPK2 (PubMed : 15004007, PubMed : 15764709). Negative regulator of the canonical Wnt/beta-catenin signaling pathway (PubMed : 12482967). Binds to and phosphorylates TCF7L2/TCF4 and LEF1, promoting the dissociation of the TCF7L2/LEF1/beta-catenin complex from DNA, as well as the ubiquitination and subsequent proteolysis of LEF1 (PubMed : 21454679). Together these effects inhibit the transcriptional activation of canonical Wnt/beta-catenin target genes (PubMed : 12482967, PubMed : 21454679). Negative regulator of the Notch signaling pathway (PubMed : 20118921). Binds to and phosphorylates NOTCH1, thereby preventing the formation of a transcriptionally active ternary complex of NOTCH1, RBPJ/RBPSUH and MAML1 (PubMed : 20118921). Negative regulator of the MYB family of transcription factors (PubMed : 15082531). Phosphorylation of MYB leads to its subsequent proteolysis while phosphorylation of MYBL1 and MYBL2 inhibits their interaction with the coactivator CREBBP (PubMed : 15082531). Other transcription factors may also be inhibited by direct phosphorylation of CREBBP itself (PubMed : 15082531). Acts downstream of IL6 and MAP3K7/TAK1 to phosphorylate STAT3, which is in turn required for activation of NLK by MAP3K7/TAK1 (PubMed : 15004007, PubMed : 15764709). Upon IL1B stimulus, cooperates with ATF5 to activate the transactivation activity of C/EBP subfamily members (PubMed : 25512613). Phosphorylates ATF5 but also stabilizes ATF5 protein levels in a kinase-independent manner (PubMed : 25512613). Acts as an inhibitor of the mTORC1 complex in response to osmotic stress by mediating phosphorylation of RPTOR, thereby preventing recruitment of the mTORC1 complex to lysosomes (PubMed : 26588989).

서열 유사성

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

Post-translational modifications

Phosphorylated on Thr-298. Intermolecular autophosphorylation on Thr-298 activates the enzyme.

Subcellular localisation

Nucleus

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제품 프로토콜

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타겟 정보

Serine/threonine-protein kinase that regulates a number of transcription factors with key roles in cell fate determination (PubMed : 12482967, PubMed : 14960582, PubMed : 15004007, PubMed : 15764709, PubMed : 20061393, PubMed : 20874444, PubMed : 21454679). Positive effector of the non-canonical Wnt signaling pathway, acting downstream of WNT5A, MAP3K7/TAK1 and HIPK2 (PubMed : 15004007, PubMed : 15764709). Negative regulator of the canonical Wnt/beta-catenin signaling pathway (PubMed : 12482967). Binds to and phosphorylates TCF7L2/TCF4 and LEF1, promoting the dissociation of the TCF7L2/LEF1/beta-catenin complex from DNA, as well as the ubiquitination and subsequent proteolysis of LEF1 (PubMed : 21454679). Together these effects inhibit the transcriptional activation of canonical Wnt/beta-catenin target genes (PubMed : 12482967, PubMed : 21454679). Negative regulator of the Notch signaling pathway (PubMed : 20118921). Binds to and phosphorylates NOTCH1, thereby preventing the formation of a transcriptionally active ternary complex of NOTCH1, RBPJ/RBPSUH and MAML1 (PubMed : 20118921). Negative regulator of the MYB family of transcription factors (PubMed : 15082531). Phosphorylation of MYB leads to its subsequent proteolysis while phosphorylation of MYBL1 and MYBL2 inhibits their interaction with the coactivator CREBBP (PubMed : 15082531). Other transcription factors may also be inhibited by direct phosphorylation of CREBBP itself (PubMed : 15082531). Acts downstream of IL6 and MAP3K7/TAK1 to phosphorylate STAT3, which is in turn required for activation of NLK by MAP3K7/TAK1 (PubMed : 15004007, PubMed : 15764709). Upon IL1B stimulus, cooperates with ATF5 to activate the transactivation activity of C/EBP subfamily members (PubMed : 25512613). Phosphorylates ATF5 but also stabilizes ATF5 protein levels in a kinase-independent manner (PubMed : 25512613). Acts as an inhibitor of the mTORC1 complex in response to osmotic stress by mediating phosphorylation of RPTOR, thereby preventing recruitment of the mTORC1 complex to lysosomes (PubMed : 26588989).
See full target information NLK

대체 명칭 보기

LAK1, NLK, Serine/threonine-protein kinase NLK, Nemo-like kinase, Protein LAK1
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Product promise

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