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AB107133

Recombinant Human NME4 protein (His tag N-Terminus)

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Recombinant Human NME4 protein (His tag N-Terminus) is a Human Full Length protein, in the 33 to 187 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

대체 명칭 보기

NM23D, NME4, NDPK-D, Nucleoside diphosphate kinase 4, nm23-H4, NDK4

1 이미지
SDS-PAGE - Recombinant Human NME4 protein (His tag N-Terminus) (AB107133)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human NME4 protein (His tag N-Terminus) (AB107133)

15% SDS-PAGE showing ab107133 (3μg).

주요 정보

Purity

>90% SDS-PAGE

ab107133 is purified using conventional chromatography techniques.

발현 시스템

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 8 Constituents: 40% Glycerol (glycerin, glycerine), 1.16% Sodium chloride, 0.316% Tris HCl

storage-buffer

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보완 제품

Primary Antibodies

AB228005

Anti-NME4 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NME4 antibody (AB228005)

  • 4
  • 2
Assay Kits

AB283968

Human Nucleoside Diphosphate Kinase D ELISA Kit

Sandwich ELISA - Human Nucleoside Diphosphate Kinase D ELISA Kit (AB283968)

  • 0
  • 0
Primary Antibodies

AB316778

Anti-CD8 alpha antibody [RM1129]

BOND RX™ Validated|20ul selling size|Recombinant|RabMAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [RM1129] (AB316778)

  • 0
  • 0
Primary Antibodies

AB133616

Anti-CD4 antibody [EPR6855]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR6855] (AB133616)

  • 5
  • 20
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"MGSSHHHHHHSSGLVPRGSHMPSWTRERTLVAVKPDGVQRRLVGDVIQRFERRGFTLVGMKMLQAPESVLAEHYQDLRRKPFYPALIRYMSSGPVVAMVWEGYNVVRASRAMIGHTDSAEAAPGTIRGDFSVHISRNVIHASDSVEGAQREIQLWFQSSELVSWADGGQHSSIHPA","proteinLength":"Full Length","predictedMolecularWeight":"19.6 kDa","actualMolecularWeight":null,"aminoAcidEnd":187,"aminoAcidStart":33,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O00746","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

NME4 also known as nucleoside diphosphate kinase D is a mitochondrial protein with a mass of approximately 17 kDa. It is found in the inner mitochondrial membrane and is part of the NDP (nucleoside diphosphate) kinase family. This protein plays a role in transferring gamma-phosphates from nucleoside triphosphates to nucleoside diphosphates facilitating essential cellular energy transactions. NME4's catalytic activity helps maintain the balance and availability of nucleotides within the mitochondria.
Biological function summary

Nucleoside diphosphate kinase activity of NME4 contributes to energy homeostasis in cells. It assists in the generation of nucleoside triphosphates necessary for mitochondrial function and integrity. NME4 operates as part of multimeric complexes within the mitochondria ensuring efficient energy metabolism. Its role extends to contributing to cellular signaling pathways by providing the necessary energy substrates for a variety of cellular responses and activities.

Pathways

Researchers have found that NME4 is involved in the regulation of the mitochondrial apoptotic pathway. It interacts with other proteins involved in apoptosis such as Bcl-2 family members by influencing the mitochondrial outer membrane permeability. Additionally NME4 participates in pathways related to oxidative phosphorylation working alongside other proteins like ATP synthase to optimize energy production and utilization. These interactions underpin its versatility in sustaining cellular energy requirements and influencing cell survival.

Dysfunction in NME4 activity has been linked to mitochondrial diseases and cancer. In particular alterations in NME4 have implications in mitochondrial myopathy where energy production becomes compromised. Similarly cancer studies have shown changes in NME4 expression affect tumor growth and survival often in conjunction with proteins involved in metabolic regulation like p53. Understanding NME4's interactions and modifications can provide insights into its role in these diseases and potential therapeutic targets.
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일반 정보

기능

Mitochondria-specific nucleoside diphosphate kinase that catalyzes the transfer of a gamma-phosphoryl group from ATP to a nucleoside diphosphate via a ping-pong mechanism involving a phosphohistidine intermediate, participating in nucleoside triphosphate homeostasis (PubMed : 10799505, PubMed : 16313181, PubMed : 18635542, PubMed : 23150663). In vitro, purine nucleoside triphosphates are much more effective as donors and acceptors than pyrimidine nucleoside triphosphates, and ribonucleosides derivatives are superior to their deoxyribonucleosides counterparts (By similarity). Associates with cardiolipin-containing mitochondrial inner membrane and locally produces ADP in the mitochondrial intermembrane space, which is directly taken up via the ADP/ATP translocase (ANT) into the matrix to stimulate respiratory ATP regeneration (PubMed : 18635542, PubMed : 24970086). Also directly provides GTP for mitochondrial GTPases, such as the dynamin-related GTPase OPA1 (PubMed : 24970086). Additionally, catalyzes the anionic phospholipid transfer, namely cardiolipin, from the mitochondrial inner membrane (IM) to the outer membrane (OM) through the formation of contact sites between IM and OM, in a nucleoside diphosphate kinase-independent manner (PubMed : 17028143, PubMed : 23150663). The switch between the nucleoside diphosphate kinase and the phospholipid transfer activity may depend on the availability and accessibility of cardiolipin and other anionic phospholipids in the IM outer leaflet and OM inner leaflet (PubMed : 23150663).

서열 유사성

Belongs to the NDK family.

Subcellular localisation

Mitochondrion intermembrane space

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제품 프로토콜

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타겟 정보

Mitochondria-specific nucleoside diphosphate kinase that catalyzes the transfer of a gamma-phosphoryl group from ATP to a nucleoside diphosphate via a ping-pong mechanism involving a phosphohistidine intermediate, participating in nucleoside triphosphate homeostasis (PubMed : 10799505, PubMed : 16313181, PubMed : 18635542, PubMed : 23150663). In vitro, purine nucleoside triphosphates are much more effective as donors and acceptors than pyrimidine nucleoside triphosphates, and ribonucleosides derivatives are superior to their deoxyribonucleosides counterparts (By similarity). Associates with cardiolipin-containing mitochondrial inner membrane and locally produces ADP in the mitochondrial intermembrane space, which is directly taken up via the ADP/ATP translocase (ANT) into the matrix to stimulate respiratory ATP regeneration (PubMed : 18635542, PubMed : 24970086). Also directly provides GTP for mitochondrial GTPases, such as the dynamin-related GTPase OPA1 (PubMed : 24970086). Additionally, catalyzes the anionic phospholipid transfer, namely cardiolipin, from the mitochondrial inner membrane (IM) to the outer membrane (OM) through the formation of contact sites between IM and OM, in a nucleoside diphosphate kinase-independent manner (PubMed : 17028143, PubMed : 23150663). The switch between the nucleoside diphosphate kinase and the phospholipid transfer activity may depend on the availability and accessibility of cardiolipin and other anionic phospholipids in the IM outer leaflet and OM inner leaflet (PubMed : 23150663).
See full target information NME4
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Product promise

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