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AB202220

Recombinant Human NMT1/NMT protein (His tag N-Terminus)

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Recombinant Human NMT1/NMT protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 496 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE.

대체 명칭 보기

NMT, NMT1, Glycylpeptide N-tetradecanoyltransferase 1, Myristoyl-CoA:protein N-myristoyltransferase 1, Peptide N-myristoyltransferase 1, Protein-lysine myristoyltransferase NMT1, HsNMT1, NMT 1, Type I N-myristoyltransferase

1 이미지
SDS-PAGE - Recombinant Human NMT1/NMT protein (His tag N-Terminus) (AB202220)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human NMT1/NMT protein (His tag N-Terminus) (AB202220)

SDS-PAGE analysis of Recombinant Human NMT1/NMT protein (ab202220), under reducing conditions. Proteins visualized by coomassie blue stain.

Lane 1 : Molecular Weight Standards

Lane 2 : 3 μg Recombinant Human NMT1/NMT protein

주요 정보

Purity

>95% SDS-PAGE

ab202220 was purified using conventional chromatography techniques.

발현 시스템

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 7.4 Constituents: PBS

storage-buffer

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보완 제품

Reagents, Controls & Accessories

AB325995

Prestained Protein Ladder - Broad molecular weight (11-250 kDa, 5-color, 11 bands)

SDS-PAGE - Prestained Protein Ladder - Broad molecular weight (11-250 kDa, 5-color, 11 bands) (AB325995)

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Proteins & Peptides

AB63996

Recombinant HIV1 Nef protein

SDS-PAGE - Recombinant HIV1 Nef protein (AB63996)

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  • 0
Primary Antibodies

AB186123

Anti-NMT1/NMT antibody

Immunoprecipitation - Anti-NMT1/NMT antibody (AB186123)

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  • 0
Proteins & Peptides

AB307479

Recombinant Human Factor D/CFD Protein (His tag) (Active)

Premium Bioactive

Functional Studies - Recombinant Human Factor D/CFD Protein (His tag) (Active) (AB307479)

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Assay Kits

AB283540

Mouse FRZB ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Mouse FRZB ELISA Kit (AB283540)

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  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

This product was previously labelled as NMT1
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서열 정보

[{"linker":null,"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMADESETAVKPPAPPLPQMMEGNGNGHEHCSDCENEEDNSYNRGGLSPANDTGAKKKKKKQKKKKEKGSETDSAQDQPVKMNSLPAERIQEIQKAIELFSVGQGPAKTMEEASKRSYQFWDTQPVPKLGEVVNTHGPVEPDKDNIRQEPYTLPQGFTWDALDLGDRGVLKELYTLLNENYVEDDDNMFRFDYSPEFLLWALRPPGWLPQWHCGVRVVSSRKLVGFISAIPANIHIYDTEKKMVEINFLCVHKKLRSKRVAPVLIREITRRVHLEGIFQAVYTAGVVLPKPVGTCRYWHRSLNPRKLIEVKFSHLSRNMTMQRTMKLYRLPETPKTAGLRPMETKDIPVVHQLLTRYLKQFHLTPVMSQEEVEHWFYPQENIIDTFVVENANGEVTDFLSFYTLPSTIMNHPTHKSLKAAYSFYNVHTQTPLLDLMSDALVLAKMKGFDVFNALDLMENKTFLEKLKFGIGDGNLQYYLYNWKCPSMGAEKVGLVLQ","proteinLength":"Full Length","predictedMolecularWeight":"59.2 kDa","actualMolecularWeight":null,"aminoAcidEnd":496,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P30419","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

N-myristoyltransferase 1 often referred to as NMT1 or NMT is an enzyme essential for catalyzing the transfer of myristate a 14-carbon saturated fatty acid to the N-terminal glycine residue of substrate proteins. This post-translational modification is called myristoylation and is necessary for the proper functioning of many proteins. NMT1 has a molecular mass of approximately 50 kDa and is widely expressed in eukaryotic cells prominently in the cytoplasm and sometimes associated with intracellular membranes.
Biological function summary

NMT1 modifies proteins by attaching myristic acid influencing their membrane-binding capability stability and function. It does not form part of a larger complex but rather acts individually to regulate protein activity through myristoylation. This modification is important for the localization and activation of proteins involved in various cellular processes including signal transduction and apoptosis.

Pathways

N-myristoyltransferase 1 plays a role in several key biological pathways. Myristoylation mediated by NMT1 regulates signal transduction pathways such as the Src family kinases and G-protein signaling. Through these pathways NMT1 interacts with key proteins like Src a tyrosine kinase involved in cell growth and differentiation and Gα proteins which are part of the G-protein coupled receptor signaling pathway.

Alterations in NMT1 activity are associated with various cancers and infectious diseases. For instance overexpression of NMT1 has been observed in colorectal cancer potentially linked through its interactions with cancer-related proteins like Src. Additionally the enzyme is important for the survival of certain pathogens which require myristoylation for their life cycle suggesting that NMT1 could be a target for antimicrobial drug development.
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일반 정보

기능

Adds a myristoyl group to the N-terminal glycine residue of certain cellular and viral proteins (PubMed : 22865860, PubMed : 25255805, PubMed : 32686708, PubMed : 34999170, PubMed : 9353336, PubMed : 9506952). Also able to mediate N-terminal lysine myristoylation of proteins : catalyzes myristoylation of ARF6 on both 'Gly-2' and 'Lys-3' (PubMed : 32103017, PubMed : 32111831). Lysine myristoylation is required to maintain ARF6 on membranes during the GTPase cycle (PubMed : 32103017).

서열 유사성

Belongs to the NMT family.

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제품 프로토콜

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타겟 정보

Adds a myristoyl group to the N-terminal glycine residue of certain cellular and viral proteins (PubMed : 22865860, PubMed : 25255805, PubMed : 32686708, PubMed : 34999170, PubMed : 9353336, PubMed : 9506952). Also able to mediate N-terminal lysine myristoylation of proteins : catalyzes myristoylation of ARF6 on both 'Gly-2' and 'Lys-3' (PubMed : 32103017, PubMed : 32111831). Lysine myristoylation is required to maintain ARF6 on membranes during the GTPase cycle (PubMed : 32103017).
See full target information NMT1
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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