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AB112416

Recombinant Human NSMase2 protein (GST tag N-Terminus)

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(1 출판물)

Recombinant Human NSMase2 protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 655 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

대체 명칭 보기

Sphingomyelin phosphodiesterase 3, Neutral sphingomyelinase 2, Neutral sphingomyelinase II, nSMase-2, nSMase2, SMPD3

1 이미지
SDS-PAGE - Recombinant Human NSMase2 protein (GST tag N-Terminus) (AB112416)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human NSMase2 protein (GST tag N-Terminus) (AB112416)

12.5% SDS-PAGE stained with Coomassie Blue.

주요 정보

발현 시스템

Wheat germ

Tags

GST tag N-Terminus

Applications

WB, ELISA, SDS-PAGE

applications

Biologically active

No

Accession

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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보완 제품

Primary Antibodies

AB85017

Anti-NSMase2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-NSMase2 antibody (AB85017)

  • 4
  • 2
Proteins & Peptides

AB109845

Recombinant Human VPS24 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human VPS24 protein (His tag N-Terminus) (AB109845)

  • 0
  • 0
Primary Antibodies

AB255818

Anti-Caspase-7 antibody [EPR22839-12]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-Caspase-7 antibody [EPR22839-12] (AB255818)

  • 0
  • 0
Proteins & Peptides

AB101195

Recombinant Human NCBP2 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human NCBP2 protein (His tag N-Terminus) (AB101195)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

This product is useful for Antibody Production and Protein Array.
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서열 정보

[{"linker":null,"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":"98.12 kDa","actualMolecularWeight":null,"aminoAcidEnd":655,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q9NY59","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Proprietary technique
배송 시 보관 조건
Dry Ice
적절한 단기 보관 조건
-80°C
적절한 장기 보관 조건
-80°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

NSMase2 also known as neutral sphingomyelinase 2 is an enzyme with a molecular mass of approximately 97 kDa. It catalyzes the hydrolysis of sphingomyelin into ceramide and phosphorylcholine. NSMase2 is expressed in various tissues like brain liver and kidney. The enzyme is membrane-associated where it plays a role in the regulation of sphingolipid metabolism.
Biological function summary

NSMase2 influences many cellular processes by controlling levels of bioactive lipids. It is an important participant in the ceramide production pathway impacting cell signaling and apoptosis. While not usually part of large complexes its activity modulates lipid raft composition influencing membrane-associated signaling pathways.

Pathways

NSMase2 contributes significantly to the sphingolipid metabolic pathway by generating ceramide which acts as a second messenger in various cellular responses. Notably it interfaces with the MAPK pathway affecting signal transduction related to stress and inflammation. It has functional relations with proteins like PKC which is involved in cell survival and apoptosis processes.

NSMase2 has been linked to neurodegenerative diseases like Alzheimer's disease and the inflammatory disorder atherosclerosis. Dysregulation in NSMase2 activity can result in altered ceramide levels contributing to these conditions. Additionally its interaction with TNFα a pro-inflammatory cytokine exemplifies its involvement in inflammatory responses and related diseases.
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일반 정보

기능

Catalyzes the hydrolysis of sphingomyelin to form ceramide and phosphocholine. Ceramide mediates numerous cellular functions, such as apoptosis and growth arrest, and is capable of regulating these 2 cellular events independently. Also hydrolyzes sphingosylphosphocholine. Regulates the cell cycle by acting as a growth suppressor in confluent cells. Probably acts as a regulator of postnatal development and participates in bone and dentin mineralization (PubMed : 10823942, PubMed : 14741383, PubMed : 15051724). Binds to anionic phospholipids (APLs) such as phosphatidylserine (PS) and phosphatidic acid (PA) that modulate enzymatic activity and subcellular location. May be involved in IL-1-beta-induced JNK activation in hepatocytes (By similarity). May act as a mediator in transcriptional regulation of NOS2/iNOS via the NF-kappa-B activation under inflammatory conditions (By similarity).

서열 유사성

Belongs to the neutral sphingomyelinase family.

Post-translational modifications

Palmitoylated, palmitoylation-deficient proteins are targeted for lysosomal degradation.

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제품 프로토콜

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타겟 정보

Catalyzes the hydrolysis of sphingomyelin to form ceramide and phosphocholine. Ceramide mediates numerous cellular functions, such as apoptosis and growth arrest, and is capable of regulating these 2 cellular events independently. Also hydrolyzes sphingosylphosphocholine. Regulates the cell cycle by acting as a growth suppressor in confluent cells. Probably acts as a regulator of postnatal development and participates in bone and dentin mineralization (PubMed : 10823942, PubMed : 14741383, PubMed : 15051724). Binds to anionic phospholipids (APLs) such as phosphatidylserine (PS) and phosphatidic acid (PA) that modulate enzymatic activity and subcellular location. May be involved in IL-1-beta-induced JNK activation in hepatocytes (By similarity). May act as a mediator in transcriptional regulation of NOS2/iNOS via the NF-kappa-B activation under inflammatory conditions (By similarity).
See full target information Sphingomyelin phosphodiesterase 3
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 99:921-930 PubMed29710492

2018

Shufeng Jiedu Capsule and its active ingredients induce apoptosis, inhibit migration and invasion, and enhances doxorubicin therapeutic efficacy in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jufeng Xia,Ling Rong,Tatsuo Sawakami,Yoshinori Inagaki,Peipei Song,Kiyoshi Hasegawa,Yoshihiro Sakamoto,Wei Tang
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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