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AB113183

Recombinant Human p23 protein

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Recombinant Human p23 protein is a Human Full Length protein, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, WB.

대체 명칭 보기

P23, TEBP, PTGES3, Prostaglandin E synthase 3, Cytosolic prostaglandin E2 synthase, Hsp90 co-chaperone, Progesterone receptor complex p23, Telomerase-binding protein p23, cPGES

2 이미지
Western blot - Recombinant Human p23 protein (AB113183)
  • WB

Unknown

Western blot - Recombinant Human p23 protein (AB113183)

Lane 1:

Western blot - Recombinant Human p23 protein (ab113183) at 0.1 µg

Lane 1:

Antibody which recognises p23

true

SDS-PAGE - Recombinant Human p23 protein (AB113183)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human p23 protein (AB113183)

ab113183, at 0.5μg in lane 1, 1μg in lane 2, 2μg in lane 3 and 5μg in lane 4, staining Human p23 using SDS-PAGE. Imperial stain used.

주요 정보

Purity

>90% SDS-PAGE

Purified by multi step chromatography

발현 시스템

Escherichia coli

Tags

Tag free

Applications

SDS-PAGE, WB

applications

Biologically active

No

Accession

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

Constituents: PBS

storage-buffer

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보완 제품

Reagents, Controls & Accessories

AB313995

Prestained Protein Ladder - Mid-range molecular weight (15 - 170 kDa)

SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (15 - 170 kDa) (AB313995)

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  • 0
Proteins & Peptides

AB132957

Recombinant Human Prostaglandin E Receptor EP2/PTGER2 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human Prostaglandin E Receptor EP2/PTGER2 protein (GST tag N-Terminus) (AB132957)

  • 0
  • 0
Proteins & Peptides

AB245933

Recombinant Human AHA1 protein (His tag)

SDS-PAGE - Recombinant Human AHA1 protein (His tag) (AB245933)

  • 0
  • 0
Proteins & Peptides

AB226420

Recombinant Mouse Telomerase reverse transcriptase protein (Tagged)

SDS-PAGE - Recombinant Mouse Telomerase reverse transcriptase protein (Tagged) (AB226420)

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  • 0
Proteins & Peptides

AB198147

Recombinant human Protein C (His tag C-Terminus)

Functional Studies - Recombinant human Protein C (His tag C-Terminus) (AB198147)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":"23 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q15185","tags":[]}]
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특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Dry Ice
적절한 단기 보관 조건
-80°C
적절한 장기 보관 조건
-80°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The p23 protein also known as prostaglandin E synthase 3 weighs about 18 kDa. It functions as a co-chaperone for the heat shock protein 90 (Hsp90) complex. P23 is found in the cytoplasm and nucleus of cells and shows expression across various tissues including the brain liver and kidney. It plays a role in stabilizing the ATP-bound state of Hsp90 assisting in client protein stabilization and maturation. P23 binding to the Hsp90 complex influences its chaperone activity.
Biological function summary

P23 assists Hsp90 in protein-folding processes. As part of the Hsp90 chaperone complex p23 contributes to the correct folding and functioning of several essential client proteins. This chaperone activity is important for cellular processes like signal transduction and protein degradation. Without p23 Hsp90's efficiency in maintaining protein homeostasis can decrease highlighting the importance of their interaction in the cell.

Pathways

The chaperone actions of p23 extensively impact protein signaling pathways and stress response mechanisms. P23 and Hsp90 are important in the Akt/PI3K signaling pathway which regulates cell proliferation and survival. The complex formed by p23 and Hsp90 also involves heat shock proteins like Hsp70 which collaborates in broader cellular stress responses. These connections demonstrate how p23 integrates into cellular regulation and signal transduction networks.

P23's interaction with Hsp90 relates to cancer development and progression. Dysregulation in the p23-Hsp90 interaction can lead to increased stabilization of oncogenic client proteins contributing to tumorigenesis. Additionally p23 implication has been noted in neurodegenerative diseases like Alzheimer's where protein misfolding plays a critical role. Here p23 involvement with proteins like Tau may link its chaperone activity to the pathological protein aggregation seen in such disorders.
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일반 정보

기능

Cytosolic prostaglandin synthase that catalyzes the oxidoreduction of prostaglandin endoperoxide H2 (PGH2) to prostaglandin E2 (PGE2) (PubMed : 10922363). Molecular chaperone that localizes to genomic response elements in a hormone-dependent manner and disrupts receptor-mediated transcriptional activation, by promoting disassembly of transcriptional regulatory complexes (PubMed : 11274138, PubMed : 12077419). Facilitates HIF alpha proteins hydroxylation via interaction with EGLN1/PHD2, leading to recruit EGLN1/PHD2 to the HSP90 pathway (PubMed : 24711448).

서열 유사성

Belongs to the p23/wos2 family.

Post-translational modifications

Proteolytically cleaved by caspase-7 (CASP7) in response to apoptosis, leading to its inactivation.

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제품 프로토콜

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타겟 정보

Cytosolic prostaglandin synthase that catalyzes the oxidoreduction of prostaglandin endoperoxide H2 (PGH2) to prostaglandin E2 (PGE2) (PubMed : 10922363). Molecular chaperone that localizes to genomic response elements in a hormone-dependent manner and disrupts receptor-mediated transcriptional activation, by promoting disassembly of transcriptional regulatory complexes (PubMed : 11274138, PubMed : 12077419). Facilitates HIF alpha proteins hydroxylation via interaction with EGLN1/PHD2, leading to recruit EGLN1/PHD2 to the HSP90 pathway (PubMed : 24711448).
See full target information PTGES3
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Scientific reports 12:10568 PubMed35732702

2022

SILAC kinase screen identifies potential MASTL substrates.

Applications

Unspecified application

Species

Unspecified reactive species

Kamila A Marzec,Samuel Rogers,Rachael McCloy,Benjamin L Parker,David E James,D Neil Watkins,Andrew Burgess
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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