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AB60754

Recombinant human PAK5 protein (GST tag N-Terminus)

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(1 출판물)

Recombinant human PAK5 protein (GST tag N-Terminus) is a Human Full Length protein, expressed in Baculovirus infected Sf9 cells, with >75%, suitable for SDS-PAGE, FuncS.
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Functional Studies - Recombinant human PAK5 protein (GST tag N-Terminus) (AB60754)
  • FuncS

Unknown

Functional Studies - Recombinant human PAK5 protein (GST tag N-Terminus) (AB60754)

The specific activity of PAK5 (ab60754) was determined to be 14 nmol/min/mg as per activity assay protocol and was equivalent to 60 nmol/min/mg as per radiometric assay

Functional Studies - Recombinant human PAK5 protein (GST tag N-Terminus) (AB60754)
  • FuncS

Unknown

Functional Studies - Recombinant human PAK5 protein (GST tag N-Terminus) (AB60754)

Sample Kinase Activity Plot.

SDS-PAGE - Recombinant human PAK5 protein (GST tag N-Terminus) (AB60754)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human PAK5 protein (GST tag N-Terminus) (AB60754)

ab60754 on SDS-PAGE, MW ~130kDa.

SDS-PAGE - Recombinant human PAK5 protein (GST tag N-Terminus) (AB60754)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human PAK5 protein (GST tag N-Terminus) (AB60754)

SDS PAGE analysis of ab60754

주요 정보

Purity

>75% Densitometry

Affinity purified.

발현 시스템

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

Active

Accession

Q9P286-1

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.0038% EGTA, 0.00292% EDTA, 0.00174% PMSF

storage-buffer

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보완 제품

Reagents, Controls & Accessories

AB325995

Prestained Protein Ladder - Broad molecular weight (11-250 kDa, 5-color, 11 bands)

SDS-PAGE - Prestained Protein Ladder - Broad molecular weight (11-250 kDa, 5-color, 11 bands) (AB325995)

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  • 0
Primary Antibodies

AB110069

Anti-PAK5 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK5 antibody (AB110069)

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  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

ab204849 (AKT1 peptide) can be utilized as a substrate for assessing kinase activity
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서열 정보

[{"linker":null,"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q9P286","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification
배송 시 보관 조건
Dry Ice
적절한 단기 보관 조건
-80°C
적절한 장기 보관 조건
-80°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
True
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일반 정보

기능

Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates the proto-oncogene RAF1 and stimulates its kinase activity. Promotes cell survival by phosphorylating the BCL2 antagonist of cell death BAD. Phosphorylates CTNND1, probably to regulate cytoskeletal organization and cell morphology. Keeps microtubules stable through MARK2 inhibition and destabilizes the F-actin network leading to the disappearance of stress fibers and focal adhesions.

서열 유사성

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.

Post-translational modifications

Autophosphorylated when activated by CDC42/p21.

Subcellular localisation

Mitochondrion

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제품 프로토콜

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타겟 정보

Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates the proto-oncogene RAF1 and stimulates its kinase activity. Promotes cell survival by phosphorylating the BCL2 antagonist of cell death BAD. Phosphorylates CTNND1, probably to regulate cytoskeletal organization and cell morphology. Keeps microtubules stable through MARK2 inhibition and destabilizes the F-actin network leading to the disappearance of stress fibers and focal adhesions.
See full target information PAK5

대체 명칭 보기

KIAA1264, PAK7, PAK5, Serine/threonine-protein kinase PAK 5, p21-activated kinase 5, p21-activated kinase 7, PAK-5, PAK-7
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Clinical and translational medicine 12:e1113 PubMed36394206

2022

Alternative splicing of HSPA12A pre-RNA by SRSF11 contributes to metastasis potential of colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yao-Jie Pan,Fu-Chun Huo,Meng-Jie Kang,Bo-Wen Liu,Meng-Di Wu,Dong-Sheng Pei
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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