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AB51234

Recombinant Human Parathyroid Hormone protein (Tag Free)

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Recombinant Human Parathyroid Hormone protein (Tag Free) is a Human Full Length protein, in the 32 to 115 aa range, expressed in Escherichia coli, with >90%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, WB, ELISA.
3 이미지
Sandwich ELISA - Recombinant Human Parathyroid Hormone protein (Tag Free) (AB51234)
  • sELISA

Unknown

Sandwich ELISA - Recombinant Human Parathyroid Hormone protein (Tag Free) (AB51234)

Standard curve for Parathyroid Hormone (Analyte : ab51234); dilution range 1pg/ml to 1μg/ml using Capture Antibody Mouse monoclonal [A1/70] to Parathyroid Hormone (ab14493) at 0.2μg/ml and Detector Antibody Rabbit polyclonal to Parathyroid Hormone (ab53040) at 0.5μg/ml.

Western blot - Recombinant Human Parathyroid Hormone protein (Tag Free) (AB51234)
  • WB

Unknown

Western blot - Recombinant Human Parathyroid Hormone protein (Tag Free) (AB51234)

All lanes:

Western blot - Anti-Parathyroid Hormone antibody (<a href='/ko/products/primary-antibodies/parathyroid-hormone-antibody-ab40630'>ab40630</a>) at 1 µg/mL

All lanes:

Western blot - Recombinant Human Parathyroid Hormone protein (Tag Free) (ab51234) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 13 kDa

true

Exposure time: 4min

SDS-PAGE - Recombinant Human Parathyroid Hormone protein (Tag Free) (AB51234)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Parathyroid Hormone protein (Tag Free) (AB51234)

ab51234 on 15%SDS-PAGE

주요 정보

Purity

>90% SDS-PAGE

Human Parathyroid Hormone(amino acids 32-115) was overexpressed in E. coli and purified to apparent homogeneity by using conventional column chromatography techniques.

Endotoxin 레벨

< 1 EU/µg

발현 시스템

Escherichia coli

Tags

Tag free

Applications

SDS-PAGE, WB, ELISA

applications

Biologically active

No

Accession

P01270-1

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 7.4 Constituents: PBS, 0.0584% EDTA

storage-buffer

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보완 제품

Reagents, Controls & Accessories

AB325997

Prestained Protein Ladder - Extra broad molecular weight (3-250 kDa, 5-color)

SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (3-250 kDa, 5-color) (AB325997)

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Assay Kits

AB230931

Human PTH ELISA Kit

Recombinant|SimpleStep

ELISA - Human PTH ELISA Kit (AB230931)

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  • 0
Proteins & Peptides

AB131754

Recombinant Human Gastrin protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human Gastrin protein (GST tag N-Terminus) (AB131754)

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  • 0
Biochemicals

AB269813

Parathyroid hormone (1-34) (human)

Chemical Structure - Parathyroid hormone (1-34) (human) (AB269813)

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Primary Antibodies

AB268111

Anti-Parathyroid Hormone antibody [PTH/2295R]

Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parathyroid Hormone antibody [PTH/2295R] (AB268111)

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  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>ab51234 can be used as a WB positive control in conjunction with <a href='/ko/products/primary-antibodies/parathyroid-hormone-antibody-ab40630'>ab40630</a>.</p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Dilution range 1pg/ml to 1μg/ml using Capture Antibody Mouse monoclonal [A1/70] to Parathyroid Hormone (<a href='/ko/products/primary-antibodies/parathyroid-hormone-antibody-a1-70-ab14493'>ab14493</a>) at 0.2μg/ml and Detector Antibody Rabbit polyclonal to Parathyroid Hormone (<a href='/ko/products/unavailable/parathyroid-hormone-antibody-ab53040'>ab53040</a>) at 0.5μg/ml.</p>" } } }
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서열 정보

[{"linker":null,"sequence":"MSVSEIQLMHNLGKHLNSMERVEWLRKKLQDVHNFVALGAPLAPRDAGSQRPRKKEDNVLVESHEKSLGEADKADVNVLTKAKSQ","proteinLength":"Full Length","predictedMolecularWeight":"9.55 kDa","actualMolecularWeight":null,"aminoAcidEnd":115,"aminoAcidStart":32,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P01270","tags":[]}]
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특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Parathyroid hormone (PTH) also known as the parathyrin is a hormone protein with a molecular mass of about 9.4 kDa. It is synthesized and secreted by the chief cells of the parathyroid glands. PTH plays an important role in regulating calcium levels in the blood. Its molecular action involves binding to PTH receptors which are mainly located in bones and kidneys to increase calcium concentration by promoting its release from bones and reabsorption in the kidneys. This hormone is also present in the bloodstream as a parathyroid hormone peptide.
Biological function summary

PTH regulates calcium and phosphate metabolism in the body. It does not function as part of a larger complex but acts independently to control these important minerals. The hormone affects osteoclast activity in bones enhancing calcium and phosphate release. Additionally in the kidneys it reduces phosphate reabsorption while increasing calcium reabsorption. This balance maintained by PTH is essential for bone health and metabolic functions often associated with parathyroid hormone-related proteins in its activity.

Pathways

PTH is integral to the calcium signaling pathway and the renal phosphate reabsorption pathway. PTH works in concert with vitamin D to enhance intestinal calcium absorption. Calcitriol the active form of vitamin D depends on PTH for its synthesis in the kidneys linking the two in calcium homeostasis. Through these pathways PTH also interacts with the calcitonin hormone to moderate calcium levels creating a balance between mineral deposition and resorption in bones.

PTH is closely associated with hyperparathyroidism and hypoparathyroidism known as pan hypoparathyroidism when complete underactivity of the parathyroid glands occurs. In hyperparathyroidism an overproduction of PTH leads to high blood calcium levels and associated symptoms. Conversely hypoparathyroidism results in low calcium levels due to insufficient PTH secretion. Both conditions affect calcium metabolism and relate to proteins like calcium-sensing receptor and PTH-related peptide highlighting how critical PTH's function is in endocrine and metabolic health.
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일반 정보

기능

Parathyroid hormone elevates calcium level by dissolving the salts in bone and preventing their renal excretion (PubMed : 11604398, PubMed : 35932760). Acts by binding to its receptor, PTH1R, activating G protein-coupled receptor signaling (PubMed : 18375760, PubMed : 35932760). Stimulates [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells (PubMed : 21076856).

서열 유사성

Belongs to the parathyroid hormone family.

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제품 프로토콜

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타겟 정보

Parathyroid hormone elevates calcium level by dissolving the salts in bone and preventing their renal excretion (PubMed : 11604398, PubMed : 35932760). Acts by binding to its receptor, PTH1R, activating G protein-coupled receptor signaling (PubMed : 18375760, PubMed : 35932760). Stimulates [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells (PubMed : 21076856).
See full target information Parathyroid hormone

대체 명칭 보기

Parathyroid hormone, PTH, Parathormone, Parathyrin
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Microbial cell factories 23:273 PubMed39390566

2024

Secretion of the human parathyroid hormone through a microcin type I secretion system in Escherichia coli.

Applications

Unspecified application

Species

Unspecified reactive species

Valeria Flórez,Juan Marizcurrena,Magela Laviña,María F Azpiroz
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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