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AB193474

Recombinant Human PARP12 protein (6x His tag N-Terminus + GST tag N-Terminus)

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Recombinant Human PARP12 protein (6x His tag N-Terminus + GST tag N-Terminus) is a Human Fragment protein, in the 500 to 701 aa range, expressed in Baculovirus infected Sf9 cells, with >98%, suitable for SDS-PAGE.

대체 명칭 보기

ZC3HDC1, PARP12, Protein mono-ADP-ribosyltransferase PARP12, ADP-ribosyltransferase diphtheria toxin-like 12, Poly [ADP-ribose] polymerase 12, Zinc finger CCCH domain-containing protein 1, ARTD12, PARP-12

1 이미지
SDS-PAGE - Recombinant Human PARP12 protein (6x His tag N-Terminus + GST tag N-Terminus) (AB193474)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human PARP12 protein (6x His tag N-Terminus + GST tag N-Terminus) (AB193474)

Lane 1 : PARP12, Lane 2 : Marker. Purity : ≥98%, MW : 51 kDa

주요 정보

Purity

>98% SDS-PAGE

Affinity purified.

발현 시스템

Baculovirus infected Sf9 cells

Tags

6x His tag N-Terminus GST tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.63% Tris HCl, 0.58% Sodium chloride, 0.25% Glutathione, 0.04% Sorbitan monolaurate, ethoxylated, 0.02% Potassium chloride

storage-buffer

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보완 제품

Reagents, Controls & Accessories

AB234592

Prestained Protein Ladder - Extra broad molecular weight (6.5 - 270 kDa)

SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (6.5 - 270 kDa) (AB234592)

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Primary Antibodies

AB241967

Anti-PARP12 antibody

Immunoprecipitation - Anti-PARP12 antibody (AB241967)

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Assay Kits

AB102500

Hydrogen Peroxide Assay Kit

Functional Studies - Hydrogen Peroxide Assay Kit (AB102500)

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Proteins & Peptides

AB158632

Recombinant Human HADHB protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human HADHB protein (GST tag N-Terminus) (AB158632)

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Biochemicals

AB145597

Bisbenzimide H 33342 (Hoechst 33342), adenine-thymine selective DNA fluorescent stain

Chemical Structure - Bisbenzimide H 33342 (Hoechst 33342), adenine-thymine selective DNA fluorescent stain (AB145597)

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  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"","proteinLength":"Fragment","predictedMolecularWeight":"51 kDa","actualMolecularWeight":null,"aminoAcidEnd":701,"aminoAcidStart":500,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q9H0J9","tags":[{"tag":"6x His","terminus":"N-Terminus"},{"tag":"GST","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification
배송 시 보관 조건
Dry Ice
적절한 단기 보관 조건
-80°C
적절한 장기 보관 조건
-80°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

PARP12 or poly (ADP-ribose) polymerase family member 12 plays a role in post-translational modifications within cells. It functions in mono-ADP-ribosylation a process which affects various proteins' activities by adding ADP-ribose to them. This protein has a calculated mass of approximately 78 kDa. It expresses in various tissues including the liver and immune cells contributing to cellular responses and defense mechanisms.
Biological function summary

Mono-ADP-ribosylation modulates cellular activities affecting processes like viral response and inflammation. PARP12 is part of the broader PARP protein family known for roles in DNA repair and cellular stress responses though PARP12 focuses more on antiviral and stress-related pathways. It does not typically engage in large multi-protein complexes but interacts closely with other molecules within its signaling network to execute its functions.

Pathways

Interactions within antiviral defense mechanisms and inflammation regulation highlight the role of PARP12. It contributes to pathways like the Interferon signaling response enhancing the cell's ability to combat viral infections. Interactions with other PARP family members such as PARP1 and PARP5 further integrate its function into broader cellular immune pathways ensuring a coordinated response to external stressors.

PARP12's involvement predominantly impacts viral infections and inflammatory conditions. It relates to viral response pathways such as those engaged in HIV replication repression highlighting its significance in infectious disease contexts. Furthermore PARP12 connects with proteins like PARP13 in modulating processes involved in immune responses offering insights into managing inflammation-related diseases.
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일반 정보

기능

Mono-ADP-ribosyltransferase that mediates mono-ADP-ribosylation of target proteins (PubMed : 25043379, PubMed : 34969853). Acts as an antiviral factor by cooperating with PARP11 to suppress Zika virus replication (PubMed : 34187568). Displays anti-alphavirus activity during IFN-gamma immune activation by directly ADP-ribosylating the alphaviral non-structural proteins nsP3 and nsP4 (PubMed : 39888989). Acts as a component of the PRKD1-driven regulatory cascade that selectively controls a major branch of the basolateral transport pathway by catalyzing the MARylation of GOLGA1 (PubMed : 34969853). Acts also as a key regulator of mitochondrial function, protein translation, and inflammation. Inhibits PINK1/Parkin-dependent mitophagy and promotes cartilage degeneration by inhibiting the ubiquitination and SUMOylation of MFN1/2 by upregulating ISG15 and ISGylation (PubMed : 39465252).

서열 유사성

Belongs to the ARTD/PARP family.

Post-translational modifications

Auto-mono-ADP-ribosylated.. Phosphorylated by PRKD1.

Subcellular localisation

Nucleus

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제품 프로토콜

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타겟 정보

Mono-ADP-ribosyltransferase that mediates mono-ADP-ribosylation of target proteins (PubMed : 25043379, PubMed : 34969853). Acts as an antiviral factor by cooperating with PARP11 to suppress Zika virus replication (PubMed : 34187568). Displays anti-alphavirus activity during IFN-gamma immune activation by directly ADP-ribosylating the alphaviral non-structural proteins nsP3 and nsP4 (PubMed : 39888989). Acts as a component of the PRKD1-driven regulatory cascade that selectively controls a major branch of the basolateral transport pathway by catalyzing the MARylation of GOLGA1 (PubMed : 34969853). Acts also as a key regulator of mitochondrial function, protein translation, and inflammation. Inhibits PINK1/Parkin-dependent mitophagy and promotes cartilage degeneration by inhibiting the ubiquitination and SUMOylation of MFN1/2 by upregulating ISG15 and ISGylation (PubMed : 39465252).
See full target information PARP12
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of virology 96:e0151621 PubMed35297669

2022

Sindbis Macrodomain Poly-ADP-Ribose Hydrolase Activity Is Important for Viral RNA Synthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Eduardo G Aguilar,Gabrielle Paniccia,Carolina Adura,Zakary S Singer,Alison W Ashbrook,Brandon S Razooky,Charles M Rice,Margaret R MacDonald
제품이 사용된 논문 모두 보기
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Product promise

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