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AB114699

Recombinant Human PCM1 protein (GST tag N-Terminus)

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Recombinant Human PCM1 protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 530 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

대체 명칭 보기

Pericentriolar material 1 protein, PCM-1, hPCM-1, PCM1

1 이미지
SDS-PAGE - Recombinant Human PCM1 protein (GST tag N-Terminus) (AB114699)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human PCM1 protein (GST tag N-Terminus) (AB114699)

12.5% SDS-PAGE showing ab114699 at approximately 84.41 kDa stained with Coomassie Blue.

주요 정보

발현 시스템

Wheat germ

Tags

GST tag N-Terminus

Applications

WB, ELISA, SDS-PAGE

applications

Biologically active

No

Accession

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 8 Constituents: 0.79% Tris HCl, 0.3% Glutathione

storage-buffer

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보완 제품

Primary Antibodies

AB72443

Anti-PCM1 antibody

Western blot - Anti-PCM1 antibody (AB72443)

  • 4
  • 2
Primary Antibodies

AB220784

Anti-Pericentrin antibody [EPR21987] - Centrosome Marker

RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pericentrin antibody [EPR21987] - Centrosome Marker (AB220784)

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  • 2
Primary Antibodies

AB2254

Anti-Aurora B antibody

BOND RX™ Validated

Western blot - Anti-Aurora B antibody (AB2254)

  • 4
  • 36
Proteins & Peptides

AB87754

Recombinant Human CHMP2A/BC2 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human CHMP2A/BC2 protein (His tag N-Terminus) (AB87754)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>(Recombinant protein).</p>" } } }
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서열 정보

[{"linker":null,"sequence":"MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTSPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQ","proteinLength":"Full Length","predictedMolecularWeight":"84.41 kDa","actualMolecularWeight":null,"aminoAcidEnd":530,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q15154","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Proprietary technique
배송 시 보관 조건
Dry Ice
적절한 단기 보관 조건
-80°C
적절한 장기 보관 조건
-80°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

PCM1 (Pericentriolar Material 1) also known as the PCM-1 protein functions mechanically in the organization of centrosomes and microtubules. This mechanistic role is linked with its name due to its positioning around the centrosome a critical cellular structure for division. The PCM1 protein shows an approximate mass of 228 kDa. It is expressed broadly in various tissues but mainly in organs with high cellular turnover including the testis thymus and heart. Notably PCM1 serves as a scaffold protein anchoring various centrosomal proteins important for their correct assembly and positioning.
Biological function summary

The PCM1 protein plays an important role in the microtubule stabilization and cell cycle progression. This protein becomes part of the larger pericentriolar material complex which supports the necessary structural platform for centrosome and cilia function. PCM1 assists in docking other proteins like centrin centrin-binding proteins and pericentriolar satellites in the complex ensuring the intricacies of ciliary trafficking and centrosome cohesion are well-maintained. It helps in regulating processes critical to normal cell polarity and division.

Pathways

PCM1 protein integrates pathway activities relating to not only microtubule dynamism but also cellular motility. It is involved in the centrosome-cilia interface important for signal transduction and cellular homeostasis. PCM1 interacts with key pathway proteins such as CEP290 and OFD1 which are significant for ciliogenesis thereby influencing cellular responses to extracellular cues. These pathways directly tie the mechanics of PCM1 to essential biological responses including cell signaling pathways that ensure normal embryonic development and cell differentiation.

PCM1 plays a relevant role in the context of ciliopathies and certain cancers. Abnormalities or mutations in PCM1 have associations with disorders such as Bardet-Biedl Syndrome a ciliopathy characterized by malfunctioning cilia. Aberrant PCM1 expressions or mutations also have links to leukemia through its implication in the ETV6-PCM1 fusion gene. The interplay between PCM1 and proteins such as ETV6 in leukemia highlights its impact on chromosomal translocations and the potential muscular pathway misregulation leading to malignancy.
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일반 정보

기능

Required for centrosome assembly and function (PubMed : 12403812, PubMed : 15659651, PubMed : 16943179). Essential for the correct localization of several centrosomal proteins including CEP250, CETN3, PCNT and NEK2 (PubMed : 12403812, PubMed : 15659651). Required to anchor microtubules to the centrosome (PubMed : 12403812, PubMed : 15659651). Also involved in cilium biogenesis by recruiting the BBSome, a ciliary protein complex involved in cilium biogenesis, to the centriolar satellites (PubMed : 20551181, PubMed : 24121310, PubMed : 27979967). Recruits the tubulin polyglutamylase complex (TPGC) to centriolar satellites (PubMed : 34782749).

서열 유사성

Belongs to the PCM1 family.

Post-translational modifications

Ubiquitinated. Undergoes monoubiquitination catalyzed by the E3 ubiquitin-protein ligase MIB1 in proliferating cells, preventing cilia formation (PubMed:24121310). Monoubiquitination by MIB1 is inhibited in response to cellular stress, such as ultraviolet light (UV) radiation or heat shock, resulting in cilia formation initiation (PubMed:24121310).. Variant Ser-159 is phosphorylated.. Phosphorylated on multiple serine and threonine residues by DYRK3 during the G2-to-M transition, after the nuclear-envelope breakdown (PubMed:29973724). Phosphorylation by DYRK3 promotes disassembly of pericentriolar material (PubMed:29973724). Phosphorylation at Ser-372 mediated by PLK4 is required to maintain the integrity of centriolar satellites (PubMed:30804208).

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제품 프로토콜

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타겟 정보

Required for centrosome assembly and function (PubMed : 12403812, PubMed : 15659651, PubMed : 16943179). Essential for the correct localization of several centrosomal proteins including CEP250, CETN3, PCNT and NEK2 (PubMed : 12403812, PubMed : 15659651). Required to anchor microtubules to the centrosome (PubMed : 12403812, PubMed : 15659651). Also involved in cilium biogenesis by recruiting the BBSome, a ciliary protein complex involved in cilium biogenesis, to the centriolar satellites (PubMed : 20551181, PubMed : 24121310, PubMed : 27979967). Recruits the tubulin polyglutamylase complex (TPGC) to centriolar satellites (PubMed : 34782749).
See full target information PCM1
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Product promise

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