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AB271653

Recombinant human PCSK9 protein (Active) (Biotin) (His tag C-Terminus + Avi tag C-Terminus)

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Recombinant human PCSK9 protein (Active) (Biotin) is a Human Full Length protein in the 31 to 692 aa range with >=80% purity and suitable for SDS-PAGE and Functional studies.The predicted molecular weight of ab271653 protein is 73.8 kDa.

- Save time and ensure accurate results - use our recombinant Human Proprotein Convertase 9 (PCSK9 /NARC1) protein as a control
- Contains C-Terminal Avi-His, Biotin-Labeled tag
- Optimal protein bioactivity and stability

대체 명칭 보기

NARC1, PSEC0052, PCSK9, Proprotein convertase subtilisin/kexin type 9, Neural apoptosis-regulated convertase 1, Proprotein convertase 9, Subtilisin/kexin-like protease PC9, NARC-1, PC9

2 이미지
Functional Studies - Recombinant human PCSK9 protein (Active) (Biotin) (His tag C-Terminus + Avi tag C-Terminus) (AB271653)
  • FuncS

Supplier Data

Functional Studies - Recombinant human PCSK9 protein (Active) (Biotin) (His tag C-Terminus + Avi tag C-Terminus) (AB271653)

Specific activity of ab271653.

SDS-PAGE - Recombinant human PCSK9 protein (Active) (Biotin) (His tag C-Terminus + Avi tag C-Terminus) (AB271653)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human PCSK9 protein (Active) (Biotin) (His tag C-Terminus + Avi tag C-Terminus) (AB271653)

SDS-PAGE analysis of 4 μg ab271653.

주요 정보

Purity

>80% SDS-PAGE

발현 시스템

HEK 293 cells

Tags

His tag C-Terminus Avi tag C-Terminus

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

50 μL reaction mix (50 mM HEPES pH 7.4, 50 mM NaCl, 200 μM CaCl2, 0.05% Tween-20, 0.1% BSA, and 0–1500 ng biotin-labeled PCSK9) is added to wells precoated with LDLR Ectodomain. Incubate for 2 hr. Add streptavidin-labeled HRP and incubate 1 hour. Finally, add HRP chemiluminescent substrates and read luminescence.

Conjugation

Biotin

Excitation/Emission
Accession

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.48% Tris, 0.02% Potassium chloride

storage-buffer

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보완 제품

Reagents, Controls & Accessories

AB234617

Prestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa)

SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa) (AB234617)

  • 0
  • 0
Primary Antibodies

AB181142

Anti-PCSK9 antibody [EPR7627(2)]

20ul selling size|RabMAb|Recombinant

Immunoprecipitation - Anti-PCSK9 antibody [EPR7627(2)] (AB181142)

  • 0
  • 0
Assay Kits

AB215538

Mouse PCSK9 ELISA Kit

Recombinant|SimpleStep

ELISA - Mouse PCSK9 ELISA Kit (AB215538)

  • 0
  • 0
Proteins & Peptides

AB316419

Recombinant Human LDLR Protein Standard (His tag)

Sandwich ELISA - Recombinant Human LDLR Protein Standard (His tag) (AB316419)

  • 0
  • 0
Secondary Antibodies

AB98624

Goat Anti-Human IgG Fc (HRP) preadsorbed

Western blot - Goat Anti-Human IgG Fc (HRP) preadsorbed (AB98624)

  • 5
  • 2
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Ensure the validity of your result using our bioactive recombinant human PCSK9 /NARC1 ab271653 as positive control in SDS-PAGE.

The molecular weight of PCSK9 /NARC1 is 73 kDa (mature form + glycans); Prodomain: 14 kDa.

ab271653 is enzymatically biotin-labeled using Avi-tag™ technology.


Check out our protein gel staining guide for SDS-PAGE here
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서열 정보

[{"linker":null,"sequence":"QEDEDGDYEELVLALRSEEDGLAEAPEHGTTATFHRCAKDPWRLPGTYVVVLKEETHLSQSERTARRLQAQAARRGYLTKILHVFHGLLPGFLVKMSGDLLELALKLPHVDYIEEDSSVFAQSIPWNLERITPPRYRADEYQPPDGGSLVEVYLLDTSIQSDHREIEGRVMVTDFENVPEEDGTRFHRQASKCDSHGTHLAGVVSGRDAGVAKGASMRSLRVLNCQGKGTVSGTLIGLEFIRKSQLVQPVGPLVVLLPLAGGYSRVLNAACQRLARAGVVLVTAAGNFRDDACLYSPASAPEVITVGATNAQDQPVTLGTLGTNFGRCVDLFAPGEDIIGASSDCSTCFVSQSGTSQAAAHVAGIAAMMLSAEPELTLAELRQRLIHFSAKDVINEAWFPEDQRVLTPNLVAALPPSTHGAGWQLFCRTVWSAHSGPTRMATAVARCAPDEELLSCSSFSRSGKRRGERMEAQGGKLVCRAHNAFGGEGVYAIARCCLLPQANCSVHTAPPAEASMGTRVHCHQQGHVLTGCSSHWEVEDLGTHKPPVLRPRGQPNQCVGHREASIHASCCHAPGLECKVKEHGIPAPQEQVTVACEEGWTLTGCSALPGTSHVLGAYAVDNTCVVRSRDVSTTGSTSEGAVTAVAICCRSRHLAQASQELQGGGLNDIFEAQKIEWHEHHHHHH","proteinLength":"Full Length","predictedMolecularWeight":"73.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":692,"aminoAcidStart":31,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"Q8NBP7","tags":[{"tag":"His","terminus":"C-Terminus"},{"tag":"Avi","terminus":"C-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Dry Ice
적절한 단기 보관 조건
-80°C
적절한 장기 보관 조건
-80°C
보관 정보
Avoid freeze / thaw cycle|Store in the dark
True
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Proprotein convertase subtilisin/kexin type 9 (PCSK9) is a protein responsible for the regulation of cholesterol levels by binding to low-density lipoprotein receptors (LDLR) on hepatocytes. This interaction marks the LDLRs for degradation reducing the liver's ability to clear LDL cholesterol from the blood. PCSK9 has a molecular weight of approximately 74 kDa. The protein is mainly expressed in the liver but is also found in the intestine and kidneys. PCSK9 is referred to as NARC-1 standing for neural apoptosis-regulated convertase 1 highlighting its role in the liver's cholesterol management system.
Biological function summary

PCSK9 influences cholesterol homeostasis by its important role in degrading LDL receptors. It functions independently rather than as part of larger protein complexes. PCSK9 gains particular interest in therapeutic contexts where its inhibition can lead to increased LDLR levels and enhanced clearance of LDL cholesterol. Biotinylated PCSK9 and mouse PCSK9 variants provide significant tools for experimental study. Kits such as the PCSK9 ELISA kit enable detailed measurement of PCSK9 levels in blood samples providing insights into cholesterol metabolism dynamics.

Pathways

PCSK9 operates within the lipid metabolism pathway and the cholesterol biosynthesis pathway. The protein's activity affects the fate of LDL cholesterol within these pathways. It interacts with proteins such as apolipoprotein B (ApoB) which plays a central role in the structural component of LDL particles. The modulation of these pathways by PCSK9 highlights the significance of its function in maintaining cardiovascular health and managing cholesterol levels.

PCSK9 is closely linked to hypercholesterolemia and coronary artery disease. Mutations in PCSK9 can lead to autosomal dominant hypercholesterolemia due to its effect on LDL receptor degradation. Other proteins such as ApoB and LDLR are involved in these conditions tightly interacting with PCSK9's regulatory function. A better understanding of PCSK9's role offers potential therapeutic targets for cardiovascular disease interventions especially through the development of PCSK9 antibodies and PCSK9 assays that adjust cholesterol levels.
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일반 정보

기능

Crucial player in the regulation of plasma cholesterol homeostasis. Binds to low-density lipid receptor family members : low density lipoprotein receptor (LDLR), very low density lipoprotein receptor (VLDLR), apolipoprotein E receptor (LRP1/APOER) and apolipoprotein receptor 2 (LRP8/APOER2), and promotes their degradation in intracellular acidic compartments (PubMed : 18039658). Acts via a non-proteolytic mechanism to enhance the degradation of the hepatic LDLR through a clathrin LDLRAP1/ARH-mediated pathway. May prevent the recycling of LDLR from endosomes to the cell surface or direct it to lysosomes for degradation. Can induce ubiquitination of LDLR leading to its subsequent degradation (PubMed : 17461796, PubMed : 18197702, PubMed : 18799458, PubMed : 22074827). Inhibits intracellular degradation of APOB via the autophagosome/lysosome pathway in a LDLR-independent manner. Involved in the disposal of non-acetylated intermediates of BACE1 in the early secretory pathway (PubMed : 18660751). Inhibits epithelial Na(+) channel (ENaC)-mediated Na(+) absorption by reducing ENaC surface expression primarily by increasing its proteasomal degradation. Regulates neuronal apoptosis via modulation of LRP8/APOER2 levels and related anti-apoptotic signaling pathways.

서열 유사성

Belongs to the peptidase S8 family.

Post-translational modifications

Cleavage by furin and PCSK5 generates a truncated inactive protein that is unable to induce LDLR degradation.. Undergoes autocatalytic cleavage in the endoplasmic reticulum to release the propeptide from the N-terminus and the cleavage of the propeptide is strictly required for its maturation and activation. The cleaved propeptide however remains associated with the catalytic domain through non-covalent interactions, preventing potential substrates from accessing its active site. As a result, it is secreted from cells as a propeptide-containing, enzymatically inactive protein.. Phosphorylation protects the propeptide against proteolysis.

Subcellular localisation

Endosome

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제품 프로토콜

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타겟 정보

Crucial player in the regulation of plasma cholesterol homeostasis. Binds to low-density lipid receptor family members : low density lipoprotein receptor (LDLR), very low density lipoprotein receptor (VLDLR), apolipoprotein E receptor (LRP1/APOER) and apolipoprotein receptor 2 (LRP8/APOER2), and promotes their degradation in intracellular acidic compartments (PubMed : 18039658). Acts via a non-proteolytic mechanism to enhance the degradation of the hepatic LDLR through a clathrin LDLRAP1/ARH-mediated pathway. May prevent the recycling of LDLR from endosomes to the cell surface or direct it to lysosomes for degradation. Can induce ubiquitination of LDLR leading to its subsequent degradation (PubMed : 17461796, PubMed : 18197702, PubMed : 18799458, PubMed : 22074827). Inhibits intracellular degradation of APOB via the autophagosome/lysosome pathway in a LDLR-independent manner. Involved in the disposal of non-acetylated intermediates of BACE1 in the early secretory pathway (PubMed : 18660751). Inhibits epithelial Na(+) channel (ENaC)-mediated Na(+) absorption by reducing ENaC surface expression primarily by increasing its proteasomal degradation. Regulates neuronal apoptosis via modulation of LRP8/APOER2 levels and related anti-apoptotic signaling pathways.
See full target information PCSK9
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Product promise

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