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AB198457

Recombinant human PDE2A protein (DDDDK tag N-Terminus)

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Recombinant human PDE2A protein (DDDDK tag N-Terminus) is a Human Full Length protein, in the 2 to 941 aa range, expressed in Baculovirus infected Sf9 cells, with >88%, suitable for SDS-PAGE, FuncS.

대체 명칭 보기

Cyclic GMP-stimulated phosphodiesterase, CGS-PDE, cGSPDE, PDE2A

2 이미지
Functional Studies - Recombinant human PDE2A protein (DDDDK tag N-Terminus) (AB198457)
  • FuncS

Unknown

Functional Studies - Recombinant human PDE2A protein (DDDDK tag N-Terminus) (AB198457)

Specific activity of ab198457 was ≥ 7577 pmol/min/μg.

SDS-PAGE - Recombinant human PDE2A protein (DDDDK tag N-Terminus) (AB198457)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human PDE2A protein (DDDDK tag N-Terminus) (AB198457)

SDS-PAGE analysis of ab198457.

Lane 1 : 6 μg ab198457
Lane 2 : Protein marker

주요 정보

Purity

>88% SDS-PAGE

Affinity purified.

발현 시스템

Baculovirus infected Sf9 cells

Tags

DDDDK tag N-Terminus

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

Specific Activity: ≥ 7577 pmol/min/μg
Assay Conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.05% Tween-20, 200 μM cAMP, and ab198457 at 37°C for 20 min. Quantified by 5-nucleotidase cleaving the 5-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.

Accession

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.72% Sodium chloride, 0.71% Tris HCl, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.02% Potassium chloride

storage-buffer

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"GQACGHSILCRSQQYPAARPAEPRGQQVFLKPDEPPPPPQPCADSLQDALLSLGSVIDISGLQRAVKEALSAVLPRVETVYTYLLDGESQLVCEDPPHELPQEGKVREAIISQKRLGCNGLGFSDLPGKPLARLVAPLAPDTQVLVMPLADKEAGAVAAVILVHCGQLSDNEEWSLQAVEKHTLVALRRVQVLQQRGPREAPRAVQNPPEGTAEDQKGGAAYTDRDRKILQLCGELYDLDASSLQLKVLQYLQQETRASRCCLLLVSEDNLQLSCKVIGDKVLGEEVSFPLTGCLGQVVEDKKSIQLKDLTSEDVQQLQSMLGCELQAMLCVPVISRATDQVVALACAFNKLEGDLFTDEDEHVIQHCFHYTSTVLTSTLAFQKEQKLKCECQALLQVAKNLFTHLDDVSVLLQEIITEARNLSNAEICSVFLLDQNELVAKVFDGGVVDDESYEIRIPADQGIAGHVATTGQILNIPDAYAHPLFYRGVDDSTGFRTRNILCFPIKNENQEVIGVAELVNKINGPWFSKFDEDLATAFSIYCGISIAHSLLYKKVNEAQYRSHLANEMMMYHMKVSDDEYTKLLHDGIQPVAAIDSNFASFTYTPRSLPEDDTSMAILSMLQDMNFINNYKIDCPTLARFCLMVKKGYRDPPYHNWMHAFSVSHFCYLLYKNLELTNYLEDIEIFALFISCMCHDLDHRGTNNSFQVASKSVLAALYSSEGSVMERHHFAQAIAILNTHGCNIFDHFSRKDYQRMLDLMRDIILATDLAHHLRIFKDLQKMAEVGYDRNNKQHHRLLLCLLMTSCDLSDQTKGWKTTRKIAELIYKEFFSQGDLEKAMGNRPMEMMDREKAYIPELQISFMEHIAMPIYKLLQDLFPKAAELYERVASNREHWTKVSHKFTIRGLPSNNSLDFLDEEYEVPDLDGTRAPINGCCSLDAE","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":941,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"O00408","tags":[{"tag":"DDDDK","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification
배송 시 보관 조건
Dry Ice
적절한 단기 보관 조건
-80°C
적절한 장기 보관 조건
-80°C
보관 정보
Avoid freeze / thaw cycle
True
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

PDE2A or phosphodiesterase 2A is an enzyme involved in the breakdown of cyclic nucleotides specifically cAMP and cGMP through its phosphodiesterase activity. This protein has a mass of approximately 105 kDa and is also known as cGMP-stimulated phosphodiesterase. PDE2A is expressed in various tissues with the highest levels found in the brain heart and adrenal gland. It is essential in regulating intracellular signals making it an important player in cellular communication.
Biological function summary

PDE2A plays a significant role in modulating signaling pathways by controlling the levels of cyclic nucleotides. It does not form part of a larger complex but interacts with other proteins to exert its function. Its activity impacts processes such as smooth muscle contraction neurotransmission and cardiac function. By hydrolyzing cAMP and cGMP PDE2A influences the cellular response to external stimuli and maintains homeostasis within cells.

Pathways

Phosphodiesterase 2A significantly contributes to the cyclic nucleotide signaling pathway and the MAPK signaling pathway. By regulating cAMP and cGMP levels PDE2A influences signal transduction mechanisms linked to these pathways. It interfaces with proteins such as protein kinase A (PKA) and guanylyl cyclase affecting downstream responses that involve cell growth differentiation and apoptosis.

Abnormal PDE2A activity has implications in neurological and cardiovascular conditions. Dysregulation of PDE2A has been associated with depressive disorders and heart failure. Its relationship with other proteins such as PKA can alter neurotransmitter release in depression and affect cardiac muscle contractility in heart failure. These connections highlight PDE2A as a potential therapeutic target for managing such conditions.
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일반 정보

기능

cGMP-activated cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes (PubMed : 15938621, PubMed : 29392776, PubMed : 9210593). Has a higher efficiency with cGMP compared to cAMP (PubMed : 15938621). Plays a role in cell growth and migration (PubMed : 24705027).. Isoform PDE2A2. Regulates mitochondrial cAMP levels and respiration (By similarity). Involved in the regulation of mitochondria morphology/dynamics and apoptotic cell death via local modulation of cAMP/PKA signaling in the mitochondrion, including the monitoring of local cAMP levels at the outer mitochondrial membrane and of PKA-dependent phosphorylation of DNM1L (PubMed : 28463107).

서열 유사성

Belongs to the cyclic nucleotide phosphodiesterase family. PDE2 subfamily.

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제품 프로토콜

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타겟 정보

cGMP-activated cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes (PubMed : 15938621, PubMed : 29392776, PubMed : 9210593). Has a higher efficiency with cGMP compared to cAMP (PubMed : 15938621). Plays a role in cell growth and migration (PubMed : 24705027).. Isoform PDE2A2. Regulates mitochondrial cAMP levels and respiration (By similarity). Involved in the regulation of mitochondria morphology/dynamics and apoptotic cell death via local modulation of cAMP/PKA signaling in the mitochondrion, including the monitoring of local cAMP levels at the outer mitochondrial membrane and of PKA-dependent phosphorylation of DNM1L (PubMed : 28463107).
See full target information PDE2A
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Product promise

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