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AB61903

Recombinant human PDGFAA + PDGFBB protein

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Recombinant human PDGFAA + PDGFBB protein is a Human Full Length protein, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, FuncS.
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Functional Studies - Recombinant human PDGFAA + PDGFBB protein (AB61903)
  • FuncS

Unknown

Functional Studies - Recombinant human PDGFAA + PDGFBB protein (AB61903)

Functional analysis of ab61903

Western blot - Recombinant human PDGFAA + PDGFBB protein (AB61903)
  • WB

Unknown

Western blot - Recombinant human PDGFAA + PDGFBB protein (AB61903)

ab61903 used in Western Blot. Figure : 1 ug in each lane (-) non-reducing conditions and (+) reducing conditions in a 4-20% Tris-Glycine gel stained with Coomassie Blue. Human PDGF-AB is predicted to be a disulfide linked heterodimer with a predicted MW of 25.5 kDa.

All lanes:

Western blot - Recombinant human PDGFAA + PDGFBB protein (ab61903)

false

주요 정보

Purity

>95% SDS-PAGE

Purity greater than 98% as determined by:Analysis by RP-HPLC.Reducing and non-reducing SDS-PAGE.

발현 시스템

Escherichia coli

Tags

Tag free

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

Biological Activity: The activity is determined by the dose-dependent proliferation of mouse 3T3 indicator cells and is typically less than 1 ng/mL.

Accession

P04085-1

Animal free

No

Carrier free

No

Species

Human

Reconstitution

Reconstitute at 0.1 mg/mL in water

보관 버퍼

Constituents: 0.06% Acetic acid

storage-buffer

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보완 제품

Reagents, Controls & Accessories

AB325995

Prestained Protein Ladder - Broad molecular weight (11-250 kDa, 5-color, 11 bands)

SDS-PAGE - Prestained Protein Ladder - Broad molecular weight (11-250 kDa, 5-color, 11 bands) (AB325995)

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Proteins & Peptides

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Recombinant human PDGF B protein (Active)

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Mass Spectrometry - Recombinant human PDGF B protein (Active) (AB259425)

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Recombinant human EGF protein (Active)

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Functional Studies - Recombinant human EGF protein (Active) (AB259398)

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AB50036

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.
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서열 정보

[{"linker":null,"sequence":"alpha chain:-MSIEEAVPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATTSLN PDYREEDTGR PRESGKKRKR KRLKPT\r\nbeta chain:- MSLGSLTIAE PAMIAECKTR TEVFEISRRL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRPTQVQLRP VQVRKIGIVR KKPIFKKATV TLGDHLACKC ETVAAARPVT","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P01127","tags":[]}]
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특성 및 보관 정보

제형
Lyophilized
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
True
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The platelet-derived growth factors PDGFAA and PDGFBB are key proteins that function as homodimers or heterodimers. PDGFAA has a molecular weight of approximately 28 kDa and PDGFBB weighs about 24 kDa. Both proteins localize to various tissues including those involved in wound healing like the skin muscle and blood vessels. They bind to their respective receptor tyrosine kinases PDGF receptors α and β and initiate signaling cascades essential for cellular proliferation and differentiation.
Biological function summary

PDGFAA and PDGFBB play significant roles in cellular processes such as growth survival and motility. These proteins often operate as part of a receptor-ligand complex that mediates signal transduction across the cell membrane. This regulatory action is vital for proper development and repair of connective tissues. The interactions between PDGFAA PDGFBB and their receptors contribute to angiogenesis and tissue remodeling highlighting their importance in both normal physiology and pathological conditions.

Pathways

PDGFAA and PDGFBB are integral to the mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3-kinase (PI3K) pathways. These pathways are critical for transmitting extracellular signals into the cell promoting cellular survival and division. The PDGFAA and PDGFBB-driven pathways interact with related proteins like Ras and Akt effectively managing cellular responses to external stimuli. Their involvement in these pathways highlights their importance in regulating the balance between cell growth and apoptosis.

The dysregulation of PDGFAA and PDGFBB links to pathological conditions such as atherosclerosis and cancer. In atherosclerosis PDGFBB collaborates with other growth factors like VEGF to incite smooth muscle cell proliferation and migration contributing to plaque formation. In certain types of cancers PDGFAA and PDGFBB overexpression supports tumor growth and metastasis involving protein partners within the tumor microenvironment. Understanding these pathways may help target therapies in these and related disorders.
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일반 정보

기능

Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal lung alveolar septum formation during embryogenesis, normal development of the gastrointestinal tract, normal development of Leydig cells and spermatogenesis. Required for normal oligodendrocyte development and normal myelination in the spinal cord and cerebellum. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFB (By similarity).

서열 유사성

Belongs to the PDGF/VEGF growth factor family.

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제품 프로토콜

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타겟 정보

Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal lung alveolar septum formation during embryogenesis, normal development of the gastrointestinal tract, normal development of Leydig cells and spermatogenesis. Required for normal oligodendrocyte development and normal myelination in the spinal cord and cerebellum. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFB (By similarity).
See full target information PDGFA

Additional targets

PDGFB

대체 명칭 보기

PDGF1, PDGFA, Platelet-derived growth factor subunit A, PDGF subunit A, PDGF-1, Platelet-derived growth factor A chain, Platelet-derived growth factor alpha polypeptide, PDGF2, SIS, PDGFB, Platelet-derived growth factor subunit B, PDGF subunit B, PDGF-2, Platelet-derived growth factor B chain, Platelet-derived growth factor beta polypeptide, Proto-oncogene c-Sis
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Product promise

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