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AB271688

Recombinant Human PDK1 protein (GST tag N-Terminus)

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Recombinant Human PDK1 protein (GST tag N-Terminus) is a Human Full Length protein, in the 2 to 436 aa range, expressed in Baculovirus infected Sf9 cells, with >15%, suitable for SDS-PAGE.

대체 명칭 보기

PDHK1, PDK1, Pyruvate dehydrogenase kinase isoform 1, PDH kinase 1

1 이미지
SDS-PAGE - Recombinant Human PDK1 protein (GST tag N-Terminus) (AB271688)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human PDK1 protein (GST tag N-Terminus) (AB271688)

SDS-PAGE analysis of 1 μg ab271688.

주요 정보

Purity

>15% SDS-PAGE

Affinity purified.

발현 시스템

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.4% Tris HCl, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.05% Sorbitan monolaurate, ethoxylated

storage-buffer

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보완 제품

Proteins & Peptides

AB125602

Recombinant Human PDHA1 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human PDHA1 protein (AB125602)

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Proteins & Peptides

AB125560

Recombinant human PDK4 protein (GST tag N-Terminus)

Functional Studies - Recombinant human PDK4 protein (AB125560)

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Proteins & Peptides

AB125606

Recombinant human PDK3 protein (GST tag N-Terminus)

Functional Studies - Recombinant human PDK3 protein (AB125606)

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Proteins & Peptides

AB125592

Recombinant human PDK2 protein (GST tag N-Terminus)

Functional Studies - Recombinant human PDK2 protein (AB125592)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"RLARLLRGAALAGPGPGLRAAGFSRSFSSDSGSSPASERGVPGQVDFYARFSPSPLSMKQFLDFGSVNACEKTSFMFLRQELPVRLANIMKEISLLPDNLLRTPSVQLVQSWYIQSLQELLDFKDKSAEDAKAIYDFTDTVIRIRNRHNDVIPTMAQGVIEYKESFGVDPVTSQNVQYFLDRFYMSRISIRMLLNQHSLLFGGKGKGSPSHRKHIGSINPNCNVLEVIKDGYENARRLCDLYYINSPELELEELNAKSPGQPIQVVYVPSHLYHMVFELFKNAMRATMEHHANRGVYPPIQVHVTLGNEDLTVKMSDRGGGVPLRKIDRLFNYMYSTAPRPRVETSRAVPLAGFGYGLPISRLYAQYFQGDLKLYSLEGYGTDAVIYIKALSTDSIERLPVYNKAAWKHYNTNHEADDWCVPSREPKDMTTFRSA","proteinLength":"Full Length","predictedMolecularWeight":"71.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":436,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q15118","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification
배송 시 보관 조건
Dry Ice
적절한 단기 보관 조건
-80°C
적절한 장기 보관 조건
-80°C
보관 정보
Avoid freeze / thaw cycle
False
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일반 정보

기능

Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2 (PubMed : 7499431, PubMed : 18541534, PubMed : 22195962, PubMed : 26942675, PubMed : 17683942). This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate (PubMed : 18541534, PubMed : 22195962, PubMed : 26942675). Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia (PubMed : 18541534, PubMed : 22195962, PubMed : 26942675).

서열 유사성

Belongs to the PDK/BCKDK protein kinase family.

Post-translational modifications

Phosphorylated by constitutively activated ABL1, FGFR1, FLT3 and JAK2 (in vitro), and this may also occur in cancer cells that express constitutively activated ABL1, FGFR1, FLT3 and JAK2. Phosphorylation at Tyr-243 and Tyr-244 strongly increases kinase activity, while phosphorylation at Tyr-136 has a lesser effect (PubMed:22195962). Phosphorylated under hypoxic conditions at Thr-338 by phosphoglycerate kinase PGK1 which has an activating effect (PubMed:26942675).

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제품 프로토콜

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타겟 정보

Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2 (PubMed : 7499431, PubMed : 18541534, PubMed : 22195962, PubMed : 26942675, PubMed : 17683942). This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate (PubMed : 18541534, PubMed : 22195962, PubMed : 26942675). Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia (PubMed : 18541534, PubMed : 22195962, PubMed : 26942675).
See full target information PDK1
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Product promise

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