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AB85559

Recombinant human Peroxiredoxin 3/PRDX3 protein (Tag Free)

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Recombinant human Peroxiredoxin 3/PRDX3 protein (Tag Free) is a Human Full Length protein, in the 63 to 256 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, FuncS.
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SDS-PAGE - Recombinant human Peroxiredoxin 3/PRDX3 protein (Tag Free) (AB85559)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human Peroxiredoxin 3/PRDX3 protein (Tag Free) (AB85559)

ab85559 on 15% SDS-PAGE (3μg).

주요 정보

Purity

>95% SDS-PAGE

ab85559 was purified by using conventional chromatography techniques

발현 시스템

Escherichia coli

Tags

Tag free

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Specific activity: approximately 82-83 pmole/min/μg. Enzymatic activity was confirmed by measuring the remaining peroxide after incubation of PRDX3 and peroxide for 20 min at room temperature. Specific activity is defined as the amount of hydroperoxide that 1ug of enzyme can reduce at 25 C for 1 minute Specific activity: approximately 82-83 pmole/min/μg. Enzymatic activity was confirmed by measuring the remaining peroxide after incubation of PRDX3 and peroxide for 20 min at room temperature. Specific activity is defined as the amount of hydroperoxide that 1ug of enzyme can reduce at 25 C for 1 minute

Accession

P30048-1

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl

storage-buffer

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보완 제품

Reagents, Controls & Accessories

AB116029

Prestained Protein Ladder - Extra broad molecular weight (5 - 245 kDa)

SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (5 - 245 kDa) (AB116029)

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Proteins & Peptides

AB151823

Recombinant Human Cytochrome P450 Reductase protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human Cytochrome P450 Reductase protein (AB151823)

  • 0
  • 0
Proteins & Peptides

AB74172

Recombinant human Peroxiredoxin 1/PAG protein (His tag N-Terminus)

SDS-PAGE - Recombinant human Peroxiredoxin 1/PAG protein (His tag N-Terminus) (AB74172)

  • 0
  • 0
Proteins & Peptides

AB202203

Recombinant Human Cytochrome P450 2E1 protein (denatured) (His tag N-Terminus)

SDS-PAGE - Recombinant Human Cytochrome P450 2E1 protein (denatured) (His tag N-Terminus) (AB202203)

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  • 0
Primary Antibodies

AB128953

Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115]

20ul selling size|RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (AB128953)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Specific activity: approximately 82-83 pmole/min/µg.</p>" } } }
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제품 세부 정보

This product was previously labelled as Peroxiredoxin 3
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서열 정보

[{"linker":null,"sequence":"MPAVTQHAPYFKGTAVVNGEFKDLSLDDFKGKYLVLFFYPLDFTFVCPTEIVAFSDKANEFHDVNCEVVAVSVDSHFSHLAWINTPRKNGGLGHMNIALLSDLTKQISRDYGVLLEGSGLALRGLFIIDPNGVIKHLSVNDLPVGRSVEETLRLVKAFQYVETHGEVCPANWTPDSPTIKPSPAASKEYFQKVNQ","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":256,"aminoAcidStart":63,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P30048","tags":[]}]
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특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
True
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Peroxiredoxin 3 also known as PRDX3 is an antioxidant enzyme that plays a critical role in the reduction of hydrogen peroxide to water. This protein has a mass of around 25 kDa and belongs to the thioredoxin peroxidase family. It functions within the mitochondria and is expressed in a wide variety of tissues with higher levels in the heart liver and kidney. PRDX3 acts as a protective buffer against oxidative stress by maintaining redox balance in cells.
Biological function summary

The enzyme helps to safeguard cells from oxidative damage by reducing peroxides produced during cellular metabolism. PRDX3 participates in antioxidant defense mechanisms and may form functional complexes with other proteins to enhance its peroxidase activity. Its antioxidant properties contribute to the maintenance of mitochondrial integrity and influence cellular survival and apoptosis processes.

Pathways

PRDX3 integrates into the cellular antioxidant defense system prominently in the thioredoxin pathway. In this pathway PRDX3 synergizes with thioredoxin to neutralize harmful oxidative molecules. PRDX3's activity is interconnected with proteins such as thioredoxin and glutaredoxin which also partake in counteracting oxidative stress bolstering an organism's defense against cellular damage.

Alterations in PRDX3 activity associate with conditions like cancer and neurodegenerative disorders. Overexpression of PRDX3 has been observed in certain types of cancer suggesting a potential role in tumor growth through its influence on cell proliferation and survival. In neurodegenerative diseases dysfunctional PRDX3 contributes to neuronal injury. It interacts with proteins like DJ-1 which relate to pathways involved in Parkinson's disease indicating its impact on maintaining neuronal health.
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일반 정보

기능

Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides (PubMed : 17707404, PubMed : 29438714, PubMed : 33889951, PubMed : 7733872). Acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol (PubMed : 12492477). Required for the maintenance of physical strength (By similarity).

서열 유사성

Belongs to the peroxiredoxin family. AhpC/Prx1 subfamily.

Post-translational modifications

Phosphorylated by LRRK2; phosphorylation reduces perodixase activity.. The enzyme can be inactivated by further oxidation of the cysteine sulfenic acid (C(P)-SOH) to sulphinic acid (C(P)-SO2H) and sulphonic acid (C(P)-SO3H) instead of its condensation to a disulfide bond.. S-palmitoylated.

Subcellular localisation

Mitochondrion

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제품 프로토콜

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타겟 정보

Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides (PubMed : 17707404, PubMed : 29438714, PubMed : 33889951, PubMed : 7733872). Acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol (PubMed : 12492477). Required for the maintenance of physical strength (By similarity).
See full target information PRDX3

대체 명칭 보기

AOP1, PRDX3, Antioxidant protein 1, HBC189, Peroxiredoxin III, Peroxiredoxin-3, Protein MER5 homolog, Thioredoxin-dependent peroxiredoxin 3, AOP-1, Prx-III
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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